固定化微生物在好氧条件下同时硝化和反硝化曹国民

　　(2),,,。(3),,,。1　DobbyGS.FinchJA.Mixingcharacteristicsofindustrialflotationcolumns.Chem.Eng.Sci.,1985,40(7):1061.2　YoonRH.TheeffectofbubblesizeonfineParticleflotation.Tech-nol.Proc.,1988,7:363.3　,,.()—.,1998.23(24):40.　,,1963,1992,。,,,。、。2000-03-06固定化微生物在好氧条件下同时硝化和反硝化＊＊(29777007)。曹国民　赵庆祥　龚剑丽　张　彤(,200237)　研究了将硝化菌和反硝化菌混合包埋,利用载体对氧产生的扩散阻力在颗粒内部形成好氧区、缺氧区和厌氧区,使硝化和反硝化两个过程有机的结合在一起,在好氧条件下同时进行硝化和反硝化的新型生物脱氮技术。试验结果表明:固定化后细胞的活力回收率≥70%;混合固定的硝化菌和反硝化菌在好氧条件下进行间歇生物脱氮时至少可稳定操作22d,其间脱氮速率约为0.11kg/m3·d;单级生物脱氮的最适pH和温度分别是8.2和30℃。　生物脱氮　硝化　反硝化　固定化1　,———,,,,,。(10mg/L),,,,。,,[1～6]。,。,,,,,,25%40%[7]。,、,,。2　2.1　硝化细菌和反硝化细菌及其培养基。:(NH4)2SO4700mg/L,NaHCO31000mg/L,、;:KNO31800mg/L,CH3OH1500mg/L,。2.2　细胞的固定化方法,3000r/min15min,2。3%,4%CaCl2,、,3mm17　　　200010185DOI:10.13205/j.hjgc.2000.05.005。,0.06mol/L1h,0.3mol/L10min,[8]。2.3　模拟废水组成　(NH4)2SO4300～500mg/L,NaHCO3500～800mg/L,,CH3OH340～570mg/L,50mM、pH8.2HCl-Tris。　KNO3350～500mg/L,CH3OH250～350mg/L,,50mM、pH7.8HCl-Tris。2.4　固定化细胞的间歇试验1L(5.1cm,H50cm)(30±1℃,),15%～20%,20～30L/h,DO=3～5mg/L。,NH+4-N、NO-3-NNO-2-N。3　3.1　固定化对硝化菌和反硝化菌活力的影响1(a);1(b)。1,70%,74%。,,1　,;,,。PVA-14%[9],,。3.2　好氧条件下的同时硝化和反硝化2。2(a),NH+4-N,,24h。NH+4-N,NO-3-N,24h。NO-2-N,8h,24h2　。,NH+4-NNO-2-NNO-3-N,DO,,NO-3-N。2(b),NO-3-NNO-2-N,,36hNH+4-N、NO-x-N,NO-3-NNO-2-N。NH+4-NNO-2-NNO-3-N,,,,NO-3-NNO-2-NN2,。,2,NO-2-N,NO-2-NN2,,,。3.3　pH值对固定化细胞活力的影响3　pHpH。pH8.0～8.4,pH7.0～7.5[10]。,,pH,3。3,pH8.218　　　200010185,pH,pH。,。,[11]。,pHpH。3.4　温度对固定化细胞活力的影响BOD5,。4,4,30℃,,;30℃,;30℃。,10℃,30℃27%[12]。10℃,30℃46%,。4　3.5　固定化细胞的操作稳定性。,,(48h),,,,5。5,,3,。,11,22d。,5,0.11kg/m3·d,PSB[4]2.2。4　(1),(≥70%);,22d,0.11kg/m3·d,PSB[4]2.2。5　(2)、,,。,NO-2-N,,,。(3)pH8.230℃。1　WatanableS,OkabeY,HirataK,etal.WaterScienceTechnol,1995.31(1):195～203.2　SenPandDentelSK.WaterScienceTechnol,1998.38(1):247～254.3　Fermandez-PolancoF,RealFJandJerisJS.WaterScienceTechnol,1994.29(10-11):339～346.4　TimberlakeDL,StrandSEandWilliamsonKJ.WaterResearch,1988.12:1513～1517.5　dosSantosVAPM,BruijnesM,TramperJ,etal.ApplMicroBiotech-nol,1996.45:447～453.6　UemotoHandSaikiH.ProgBiotechnol,1996.11(immobilizedcells):622～632.7　dosSantosVAPM,TramperJandWijffelsRH.BiotechnolAnnuRev,1998.4:321～394.8　.,1992.18(1):46～51.9　LinYFandChenKC.WatSciTechnol,1993.28(7):159～164.10　..:,1992.17～30.11　BaileyJEandOllisDV.BiochemEngFunda.2nd.NewYork:Mc-Graw-HillBookCompany,1986.186.12　...:,1993.201.　,,196312。,,20。1999-12-2219　　　200010185APILOT-PLANTSCALESTUDYOFTHECATALYTICAERATION-MICROELECTROLYSISPRO-CESSFORTREATMENTOFEFFLUENTFROMDELAYEDCOKINGPLANTOFPETROLEUMRE-FINERYWangDongetal(7)………………………………………………………………………………………Abstract　A1m3/hon-sitepilot-plantstudyofcatalyticaeration-microelectrolysisprocesswasconductedtotreatthedenseeffluentfromadelayedcokingplantofpetroleumrefinery.Theperformanceandfactorsoftwowork-units,catalyticaerationandmicroelectrolysis,wereexamined,whilethescale-upeffectofthefacilitiesana-lyzedbyacomparisonwithresultsobtainedfromabench-scaletest,combiningelectrochemicalandmultiphasefluidprinciples.Thepilotprocesshasshownitsfavorableefficiencyandapplicability,totalremovalratesofS2-andCODCrwereover90%and75%respectively.Keywords　pilotplant-scalestudy,catalyticaeration,microelectrolysisandeffluentfromdelayedcokingplantEXPERIMENTALSTUDYOFTHEAPPLICATIONOFPHOTOSYNTHETICBACTERIA(PSB)TOWASTEWATERTREATMENTOFHOGGERYChenFanzhongetal(10)…………………………………Abstract　Anexperimentofwasterwatertreatmentofahoggerywasconductedusingabiological-contactoxi-dationprocessofPSBpelliclefixedbycoalcinderfiller.TheresultsshowedthatthemeanremovalratesofCODCr,NH3-N,NO-2anS2-were89.3%,80.1%,90.6%and82.4%respectively,andthemagnitudesofPSBintheef-fluentreached4.8×104germs/mL.Keywords　photosyntheticbacteria,wasterwaterofhoggeryandbench-scaleexperimentSTUDYOFDISPOSALTECHNOLOGYOFLEACHATEFROMURBANHOUSEREFUSELANDFILLYangXiaetal(12)………………………………………………………………………………………………Abstract　Adisposalprocessofdeammoniationanddenitrification-SBRbiochemicaltreatment-disinfectionbychlorideisproposedinthelightofthefeaturesofaleachatefromurbanhouserefuselandfill.Theresultsofthetestprovethataftertheprocess,theindexofNH4-N,CODCrintheleachateofdifferentperiodsareuptotheemissionstandard.Keywords　garbageleachate,ammonia-nitrogen,SBRbiochemicaltreatmentandchlorizationRESEARCHONTREATMENTPAPER-MAKINGWASTEWATERBYAIR-FLOATCOLUMNOFFILLERSDingYigangetal(15)……………………………………………………………………………………Abstract　Anair-floatcolumnoffillersisusedtotreatthepaper-makingwastewater.Itisindicatedbytheex-aminationofthedoseageofaluminiumchloride,theapparentflowratesoftheinfluentanddissolvedairwater,re-coveryandseparationsectionsofthecolumn,etcthatthedeviceandprocesshaveobviouseffectivenessonloweringCODCrandSSinthewastewaterandraisingthesolidscontentofthescumsludgeetc.Italsoshowsbythecontinu-ousteststhatthedevicehasabetterfunctionofreducingBOD5ofthewastewater.Keywords　columnoffiller,dissolvedairandpaper-makingwastewaterSIMULTANEOUSNITRIFICATIONANDDENITRIFICATIONOFIMMOBILIZEDMICROBESUNDERAEROBICCONDITIONSCaoGuominetal(17)…………………………………………………………………Abstract　Thesingle-stagebiodenitrificationtechnologyunderaerobicconditionswasstudiedbyusingtheprepara