关于好氧反硝化菌筛选方法的研究周丹丹

44　6200412ActaMicrobiologicaSinicaVol.44December　No.62004＊。Tel:[phone];Fax:[phone];E-mail:[e-mail]:(1980-),,,,。E-mail:[e-mail]:2004-04-05,:2004-07-09周丹丹　马　放＊　王弘宇　董双石(　　150090)　:,,105。TN,25TN50%。,25,24:NO3-N※NO2-N※N2,。,。:,,,:Q939.9　　:A　　:0001-6209(2004)06-0837-03　　,,,A[1]。,。,。2080。Rob-ertsonKuenen[2](Paracoccuspantotropha)、(Pseudomonasspp.)(Alcaligenesfae-calis)。,Robertson,:Frette(1997)[3]16,。Pai(1999)[4]3,T6,NH4+-N、NO2--N。,,(HyphomicrobiumX)[5]。1　材料和方法1.1　FM[6],。DM[4],。1.2　4180mm,3L(1),1、2、34。1();2();3、4SBR。1、2、3,4。1　Fig.1　Domesticationequipment1.Batchaeration;2.Sequentialaeration;3.Puddler;4.Aerationpump;5.Aerationpipe.1、23,:※※※(0.5h)※(0.5h),8h,3d1～2h,;4,:※(23h)※(0.5h)※(0.5h)。,FM,DM。COD5000mgL,(TN)500mgL,,(DO)5mgL,(25～30℃)。40d,4COD80%,TN70%,,TN100%。1、23,,40d,,,。1.3　410mL90mL、,,。,DOI:10.13343/j.cnki.wsxb.2004.06.030,DM3105。DM100mLDM,(),9,,:30℃,140rmin,40h。TN,TN。50,。1.4　,TN,50。,N2N2O,N2。,N2。2。2　Fig.2　Equipmentofsecondscreeningofaerobicdenitrifiers1.Sampleoutlet1;2.Sampleoutlet2;3.Gasinlet;4.Gasoutlet;5.Gasflowmeter;6.Tempreturemonitor;7.Oxygenjar;8.Bacterialfil-ter;9.Reacror;10.Barometer;11.Waterbath;12.Magneticstirrer;13.Aerationpipe.,2000mL,0.25μm。1000mLDM,,,3Lmin3min,N2,,,,,。,,(DO8～10mgL)。1,N2,2,NO3--N、NO2--NDO。1.5　NO3--N:;NO2--N:N-(1-)-;TN:[7];COD:CTL-12;TOC、TC、IC:SHIMADZUTOC-VCPN;(DO):YSI5000DO;(A):,721OD480;(N2):AgilentHP4890D,GS-03-106,:He20mLmin;0.08Mpa;60℃;120℃;120℃。2　结果和分析2.1　,CODTN,COD20%～40%80%,TN20%～30%70%～80%。105,TN50%25(1),40%～50%15,30%～40%10,。1　TN50%Table1　StrainsthatTNremovalratewasover50%NameofstrainsTNremovalrate%NameofstrainsTNremovalrate%NameofstrainsTNremovalrate%X3182L962C656Z3180C560Z556C379P360X654Z774C159L353X274X159P751C771Z259XT3C51XT671Z658P651XT565X556XT3A50X32622.2　2TN50%25,25。36。,:,、;X31,NO2--N,NO3--NNO2--N,NO2--NN2,X31NO2--N,NO3--NN2。3　结论和建议(1)※※(TN)※(),。。838　　　　　　　　44　3　6Fig.3　ChangeofNelementofsixstrainsA:StrainC3;B:StrainZ7;C:StrainC7;D:StrainZ31;E:StrainX2;F:StrainX31.(2),,,。(3)25,24NO2--N,NO3--N※NO2--N※N2,X31NO2--N,　　NO2-,NO2-。(4)NO3-NO2-NO2-,,NO3-NO2-。(5),,(SND),,。[1]..:,2001,140-141.[2]RobertsonLA,vanNeilEWJ,TorremansRAM,etal.Simulta-neousnitrificationanddenitrificationinaerobicchemostatculturesofThiosphaerapantotroph.ApplEnvironMcrobiol,1988,11(54):2812-2818.[3]FretteL,GejlsbjergB,WestermannP.Aerobicdenitrifiersisolatedfromanalternatingactivatedsludgesystem.FEMSMicrobiologyEcology,1997,24:363-370.[4]PaiSL,ChongNM,ChenCH.Potentialapplicationsofaerobicdenitrifiyingbacteriaasbioagentsinwastewatertreatment.Biore-sourceTechnology,1999,68:179-185.[5]MeibergJBM,BruinenbrgPM,HarderW.Effectofdissolvedox-ygentensiononthemetabolismofmethylatedaminesinHyphomi-crobiumXintheabsenceandpresenceofnitrute:aerobicdenitrifica-tion.JGenMicrobiol,1980,120:453-463.[6]　,,..:,2002:76-77.[7]《》...:,2002.StudyonScreeningMethordofAerobicDenitrifiersZHOUDan-Dan　MAFang＊　WANGHong-Yu　DONGShuang-Shi(DepartmentofEnvironmentalEngineering,HarbinIndustryandTechnologyUniversity,Harbin150090,China)Abstract:Afterdomesticatingactivatedsludgetoenrichaerobicdenitrifier,105strainswereisolatedfromit.25strainswithover50%ofTNremovalratewerescreenedbymeasuringTN.ItwasprovedthattheycanproducenitraterespirationunderaerobicconditionbymeasuringtheconcentrationofNO2--N、NO3--NandN2.Thedenitrificationprocessofthe24strainsisNO3--N※NO2--N※N2.Moreover,itwasfoundthattherewerenoobviouscompetitionfunctionbetweenNO3-andNO2-.Itisdeducedthataerobicdenitrificationcanhelptorealizeshortenedsimultaneousnitrificationanddenitrificationandnitrationspeedismorerapidviafeedbackeffect.Keywords:Aerobicdenitrifier,Aerobicdenitrification,Domesticateactivatedsludge,Shortenedsimultaneousnitrifica-tionanddenitrification＊Correspondingauthor.Tel:[phone];Fax:[phone];E-mail:[e-mail]:04_05_2004839　6: