好氧反硝化菌群的筛选及其培养条件的研究孙菲

25220106　　　　　　　　　JournalofAnhuiUniversityofTechnologyandScienceVol.25.No.2Jun.,2009:2009-11-09　:(08040102002):　(1983-),,,.:(1956-),,,,.:1672-2477(2010)02-0036-05孙　菲1,方金武2,蔡昌凤1＊,赵文玲1(1.,　241000;2.,　241002):A2/O,(DM100)7,,DO=2.5mg/L4,F4、F8、F9、F10.,4,,48hNO-3-N98.75%.4:35℃,pH=8.0,C/N=5,=25%((23)×107/mL).,NO-3-N90%96h18h.　　:;;;:X703.1　　　　:A,,[1].,,[2-4].NO-3NO-2,NO-3NO-2N2ON2[5].,[6].,.:(1),;(2),、,-;(3)OH-,pH[7].-[8-9].、,,.、、、.1　1.1　:(A2/O).:,COD4829.3mg/L,NH+4-N114.85mg/L,NO-3-N7.87mg/L,862mg/L,157mg/L,pH9.0.(DM0):10g/L,10g/L,NaCl5g/L,15～20g/L,,pH7.4～7.6,121℃20min.DM100.(FM10):13.2g/L,KNO32g/L,K2HPO40.5g/L,MgSO4·7H2O0.2g/L,FeSO4·7H2O0.05g/L,CaCl20.02g/L,10%,pH7.5～8.0,115℃30min(NO-3-N315.47mg/L).FM6060%.1.2　(1).150mLFM10,30℃、160r/min48h,10mL3.(2).0.2mLDM0DM100(3),30℃1～3d,DM0DM100,10.(3).,FM10,30℃、160r/min48h96h,NO-3-N,.NO-3-N.(4).:F3、F4、F5、F7、F8、F9、F10DM10、DM20、DM40、DM60、DM80、DM100,.:FM10,30℃、160r/min24h,12h,NO-3-N.(5).20%FM10,30℃、160r/min24h,NO-3-N.23,、4.(6).(20～40℃)、pH(6.5～9.0)、C/N(1～7)、(5%～40%),.,,FM10,pH=7.5～8.0,35℃,160r/min,DO=2.5mg/L,(2～3)×107/mL.(7).,,,,[10].721600nm,,.:20%120mLFM10,35℃,160r/min,4h,.2　2.1　10,F1～F10.1.DM0DM100.DM0F3,1～2mm;DM100,.1　DM0DM100F1(),F2,F3,1～2mm,F4,,F5,,F6,F7,F8,,F9,,F10(),2.2　(1).DM100,F7F8DM0,,.NO3-N(1),F6NO-3-N,4.16%.F1、F248h96h,NO-3-N,,·37·2　,:NO-3-N.F7+F896hNO-3-N98.71%,96hNO-3-N97%F3、F4、F5、F7、F8、F9、F10.(2).2,,NO-3-N,.60%,72h,F3、F5、F7FM60NO-3-N50%,F4、F8、F9、F1068.95%～71.48%,F4、F8、F9、F10,.2　NO-3-N/h/%NO-3-N/%F3F4F5F7F8F9F1024100.886.640.351.613.588.462.77483520.3035.1421.9020.2834.0037.0042.67726047.7668.9543.2336.6871.0068.5571.482.3　,,,.,.2,F4、F10F4z、,OD1.2.34NO-3-N90%,:F4、F10、F2z、F3z、F4z48hNO-3-N90%,F9、F872h96h.F4zNO-3-N,98.75%1.78.4,.1　NO-3-N2　3　NO-3-N2.4　(1).,、,、,[11].20～40℃.NO-3-N4.4,20～35℃,40℃,,,,.35℃,,18hNO-3-N,67.67%.(2)pH.、pH,pH,,.pHNO-3-N5.pH8.0,24hNO-3-N99.43%.pH8.5,,.,,pH,,,.(3)C/N.,,,.、,.6,C/N5,18hNO-3-N92.33%,C/N5,C/N,C/N5,NO-3-N,.(4).,NO-3-N·38·　　　　　　　　　25(7).5%～20%,.25%,18hNO-3-N89.54%,,,OD,.4　5　pH6　C/N7　2.5　　　8　,.,4:、、、[12].8,F441h,,3,.4,,,.4,.3　DM100、,..,NO-3-N,,.,35℃,pH8.0,C/N5,25%((2～3)×107/mL).,NO-3-N90%96h18h.:[1]　,.[J].,2003,28(2):173-176.[2]　,.[J].,1997,14(3):30-34.[3]　,,,.[J].,2006,25(1):34-44.[4]　ZhangM,TayJH,QianYetal.CokePlantWastewaterTreatmentbyFixedBiofilmSystemforCODandNH3-NRemoval[J].WatRes,1998,32(2):519-527.[5]　,,,.[J].,2007(2):7-12.[6]　,,,.[J].,2004,44(6):837-849.[7]　,,,.[J].,2006(7):153-155.[8]　XieSG,ZhangXJ,WangZS.IntegratedstudyonbiochemicalMechanisminbiofiltersystem[J].ActaSciCircumst,2002,22(5):557-561.[9]　DingAZ,FuJM,ShengGY.Evidenceofaerobicdenitrification[J].ChinScieBull,2000,45(3):2779-2782.[10],,,.[J].,2008,36(6):2191-2193.[11],.[J].(),2003,28(2):173-176.[12],,.[M].:,1990.(下转第79页)·39·2　,:、,,.:[1]　,,,.[M].:,2001:87-90.[2]　.[J].,2008(2):54-57.[3]　.[M].:,2002:113-116.[4]　.MCS-51[M].:.1993:517-520.DesignofthecylinderpressuredetectorbasedonSCMYANGLiang,WANGMing(AnhuiProvincialKeyLaboratoryofElectricandControl,AnhuiPolytechnicUniversity,Wuhu241000,China)Abstract:Cylinderpressureisanimportantparameterastheenginerunningnormally.Traditionalana-logpressuregaugeshaveitsowninherentdeficienciessuchaspointerswing,bigerror,etc.Forthisproblem,akindofthedigitalcylinderpressuredetectorusedAT89C52microcontrollerasthecoreisde-signed.Thevariouspartsofhardwarecircuitandthesoftwaredesignareesented.Thedesign,andhasmanyadvantagessuchassmallsizeisoflowcostandsomepracticalvalue,duetouseconventionalIC.Keywords:pressuremonitoring;SCM;A/DConverse(上接第39页)ScreeningofaerobicdenitrifyingbacteriaanddeterminationofthecultureconditionsSUNFei1,FANGJin-wu2,CAIChang-feng1,ZHAOWen-ling1(1.Coll.ofBioch.Engn.,AnhuiPolytechnicUniversity,Wuhu241000,China;2.WuhuXinxingDuctileIronPipesCo.,Ltd,Wuhu241002,China)Abstract:Sevenstrainsdenitrifyingbacteriawereisolatedbybeefextractpeptonemediummixedwiththecokedwastewater(DM100),fromHuaibeiCokingplant'sactivatedsludgeoftheA2/Osewagetreatmentstation.Aftergradientincreaseofcokingwastewater'splatedomesticationandliquiddomestication,fourstrainsaerobicdenitrifyingbacteriawithhighdenitrificationcapacityandresistancetocokingwastewaterwereisolatedinaerobiccondition.(DO=2.5mg/L),namedF4,F8,F9,F10.Thecontrastexperimentshowedthatfourmixedaerobicdenitrificationbacteriahadhigherdenitrificationcapacityandgrewfasterthansinglestrain.Underthesamecondition,fourmixedaerobicdenitrificationbacteria48hNO-3-Nremovalrateis98.75percent.Andthemostsuitablecultureconditionsofthemare:35℃,pH=8.0,C/N=5,inoculatequantityis25percent(bacteriadensityis2×107～3×107/mL).Afterconditionsopti-mization,thetimeoffourmixedaerobicdenitrificationbacteriaNO-3-Nremovalratewhenattaining90percentreducedform96hto18h.Keywords:aerobicdenitrifyingbacteria;screening;cultureconditions;cokingwastewater·79·2　,: