好氧反硝化菌株的筛选培养及其反硝化性能研究黄廷林

41　5200910西安建筑科技大学学报()J.Xi′anUniv.ofArch.&Tech.(NaturalScienceEdition)Vol.41　No.5Oct.2009黄廷林1,2,苏俊峰1,李　倩1(1.,710055;2.,710055)　:HF3.16SrDNA,NC004129Pseudomonas.-,7～8mg/L,HF3.,2.3mg/L,24h90%;pH7.0～9.0、15～30℃,,48h65%～95%.HF3,70%,.,HF3.:;;C/N:X703　　　　:A　　　　:1006-7930(2009)05-0704-04　.＊,[1-2].,[3-5],.,,,,[6-7].,.,[8].ThiosphaeraPa-ntotropha、Thaureamechernichensis、Alcal-igenesfaecalisPseudomonasnautical、Microvirgulaaerodenitrificans[9].HF3、pH、C/N,HF3,,.1　材料与方法1.1　、,.1.2(1):CH3COOHNa·3H2O/0.5g;KNO3/0.1g;K2HPO4/0.02g;MgCl2/0.01g;CaCl2/0.01g;H2O/1000ml;pH7～7.5.(2):CH3COOHNa·3H2O/0.5g;KNO3/0.05g;Na2HPO4.7H2O/0.5g;NaNO2/0.01g;MgSO4·7H2O/0.1g;18g;pH7～7.5.1.3　(1),24h,10d,1mL9mL,(2)、.＊:2008-11-10　　:2009-08-12:(50830303,50778147);863(2007AA06Z302):(1962-),,,,,:,,.DOI:10.15986/j.1006-7930.2009.05.0021.4　、,KNO3,30℃,24h,10mL,,NO3-N.,HF3.1.5　(NO3-N2.50mg/L),HF3,30℃,24h,10mL,N-(1-)-[7]NO3-NNO2-N,.1.6　[10]《》.图1　菌株HF3油镜照片Fig.1　PhotomicrographsofstrainHF31.7　NO3-N:;NO2-N:N-(1-)-[9].2　结果与讨论2.1HF3(),0.5～1μm×1.5～4μm,1.HF3:、、、、、、,、、、.2.2　16SrDNA图2　菌株HF3同相近序列采用NJ法构建的系统进化树Fig.2　PhylogenictreeofstrainHF3andsimilarsequencesinGeneBank,constructedbyNeighborJoiningMethodHF316SrDNA.,NCBIBlastnCluastx、PhyloDrawGeneBank[11],,2.2.3　,HF3,30℃.HF399%,.,HF3图3　pH值对HF3菌株好氧反硝化的影响Fig.3　InfluenceofpHonaerobicdenitrificationbystrainHF3.,,,.,,.2.4　pHpH,pH5、6、7、8、9,HF3,30℃.,3(48h,).,pHHF3.pH7～9,70%;pH7,,90%;pH7,.,HF3,.,7055　　　　　　:.,,[H+],.,HF3pH7～9.图4　温度对HF3菌株好氧反硝化的影响Fig.4　InfluenceoftemperatureonaerobicdenitrificationbystrainHF32.5　HF3,15、25、30、35℃,HF3,,4(48h,).,HF3.25～35℃HF3,,93%..,,,,..4,HF315℃65%,,.2.6C/NC/NHF3,图5　C/N对HF3菌株好氧反硝化的影响Fig.5　EffectoftheC/NratioondenitrificationperformancebystrainHF3,,C/N1.5∶1,3∶1,4.5∶1,6∶1,9∶1,5(24h,).5,HF3C/N.C/N1.5∶113.5%,C/N9.0∶1100%.,,.,,.,,.3　结　论(1)HF3.16SrDNA,NC004129Pseudomonas,,.(2)HF32.3mg/L,24h90%.(3)HF3pH7.0～9.0,15～30℃.HF3,C/N,C/N6∶1,.　References[1]　,,　./N2O[J].,2009,30(7):2105-2111.JIANGJing-yan,HUZheng-hua,HUANGYao.IsolationofHeterotrophicNitrifiers/AerobicDenitrifiersandTheirRolesinN2OProductionforDifferentIncubations[J].EnvironmentalScience,2009,30(7):2105-2111.[2]　,,,.[J].:,2001,29(12):84-88.HUANGRui-min,WUHong-hai,HUYong-you,etal.DisposalofNH3-NinMicropollutedSourceWaterfromZhujiangRiverbyBiologicalTreatment[J].JournalofSouthChinaUniversityofTechnology(NaturalScience),2001,29(12):84-88.[3]　,,,.[J].:,706　　　　　　　　　　　　　　　()　　　　　　　　412007,39(1):14-17.HUANGTing-lin,XIAOZhou-qiang,XUJin-lan,etal.Experimentonbioremediationofoil-contaminatedsoilinnorthernShannxiProvince[J].J.Xi′anUniv.ofArch.&Tech.(NaturalScienceEdition),2007,39(1):14-17.[4]　,　,,.[J].,2007,40(10):1205-1208.SUJun-feng,MAFang,WANGJi-hua,etal.NitrificationandDenitrificationCharacteristicsofNewHeterotroph-icNitrifyingBacteria[J].JournalofTianjinUniversity,2007,40(10):1205-1208.[5]　,,,.[J].:,2006,38(6):790-794.HUANGTing-lin,XIAOZhou-qiang,XUJin-lan,etal.Bioremediationofoil-contaminatedsoilunderuniforme-lectric(UE)fieldconditioninnorthShannxiProvince,China[J].Xi′anUniv.ofArch.&Tech.(NaturalScienceEdition),2006,38(6):790-794.[6]　,,,.[J].,2005,11(2):222-225.KONGQing-xin,LIJun-wen,WANGXin-we,etal.Anewscreeningmethodforaerobicdenitrificationbacteriaandisolationofanovelstrain[J].ChineseJournalofAppliedandEnvironmentalBiology,2005,11(2):222-225.[7]　BrierleyEDR,WoodM.Heterotrophicnitrificationinanacidforestsoil:isolationandcharacterisationofanitrif-yingbacterium[J].SoilBiol.Biochem.,2001,33:1403-1409.[8]　,.[J].,2004,10(4):521-525.LIUZhi-pei,LIUShuang-jiang.Advancesinthemolecularbiologyofnitrifyingmicroorganisms[J].ChineseJour-nalofAppliedandEnvironmentalBiology,2004,10(4):521-525.[9]　　,,,.[J].2007,18(11):2618-2625.WANGWei,CAIZu-cong,ZHONGWen-hui,etal.Researchadvancesinaerobicdenitrifiers[J].ChineseJour-nalofAppliedEcology,2007,18(11):2618-2625.[10]　　,,.[M].:,2002.MAFang,RENNan-qi,YaANGJi-xian.Microbiologicalexperimentsforpollutioncontrol[M].Haerbin:Ha-erbinInstituteofTechnologyPress,2002.[11]　YANGY,DUNGANRS,IBEKWEAM,etal.Effectoforganicmulchesonsoilbacterialcommunitiesoneyearafterapplication[J].BiolFertilSoils,2003,38:273-281.Isolation,identificationanddenitrifyingcharacteristicsofaerobicdenitrifyingbacteriaHUANGTing-lin1,2,SUJun-feng1,LIQian1(1.SchoolofEnvi.&Muni.Eng.,Xi′anUniversityofArchitectureandTechnology,Xi′an710055,China;2.KeyLaboratoryofNorthwestWaterResource,EnvironmentandEcologyofMinistryofEducation,Xi′an710055,China)Abstract:AbacterialstrainnamedHF3wasscreenedfromthesedimentsofsourcewaterreservoirfordenitrificationofwaterunderaerobicconditions.Accordingtomorphologicalobservation,physiologicalbiochemicalidentificationandse-quenceanalysisofthe16SrDNA,bacterialstrainHF3wasidentifiedasNC004129Pseudomonas.Byusingglucoseandpo-tassiumnitrateascarbonandnitrogensourceduringcharacterizationofthedenitrificationbacteria,denitrificationeffectofthestrainHF3werestudied.Theexperimentalresultsshowthatthenitrogenremovalratecouldreachabove90%after24hoursbycontrollingtheconcentrationofdissolvedoxygenat7～8mg/L,whentheinitialcontentofthenitrogenoftherawwaterwas2.3mg/L.Th