好氧反硝化细菌处理硝酸盐废水的研究及微生物群落结构分析

16l2(X)82JOURNALOFBASICSCIENCEANDENGINEERINGVOL16,No.lFebru28:1530(2(X)8)019:x703:A,2,,,3,2,2(1.,71(X);2.,15;3.,4372):,,5.nBf5TN90.4%91.2%94.6%95.6%97%,.5.PCRDGGE,,.d30d80%.,26,2,.:PCRDGGE;,N03-N02-NON20N:,.,,,.[[JsBR[,l,,[8.:/,;pH,;,.N,:2(X1018;:2(X)710:;973(2cB185050):(1977),,.Eu:n977518@sina.~.cn:(1963),,,.El:[e-mail].16.,,DO,,,.,.,,,5,5,.1.11.1.139,NaCI59,109,H201mL,pH7.oee7.2.1.1.2DML)NaZHp047H207.9:21.5;C10.3;MgS700.1;4.7;2;KNo3NaNo2.(L):EDrA0;zns2.2;CaC125.5:MnCI:4HZO5.06;FeSO;7HZO5.0;(NH;)6Mo70:4H201.1;CuSO;SHZO1.57:CoC126HZO1.61.1.262,,DM(KNO1L,NaN020.5L)100mM250ml,,9,.:30,160r/rain,24h.TN,TN.1.3sBR,KNoNHPo4KHZp04MgS047HZOMnso4NaAe.1.1Table1WaterqUalityofthesynthetiewastewaterLNONN02-N149.57356.7146.5103.860.078.4754.38114.466.87.51.4SBRsBR,3L,.,3.2025,12h,smin,Hh20min,30min,smin,50%,1.5DNA500LPBS,12000r/min,10min,,:31300DNA3730min,5n,.80ro%SDS,65lh,,l20(r/n,romin,1.smL.30LNaACromin,l20(r/n5n,,400L/(24:1).12xr/n5n,400L,,12rmin5n,2ooL..0.6,.20,lh.l20(r/nn,.6sh.20L.1.DNA16SDNAv31..1(l)16SrDNAV3:38GCRsls,:38GC:(5-CGCCCGCCGCGCCCCGCGCCCGTCCCGCCGCCCCCGCCCGCCTACGGGAGGCAGCAG)RS18(5ATTACCGCGGCTGCTGG).1.6.2PcR50PCR:loong20Pmol200oFNTp(lonunoFL)5L10xPCRbu,r(MgCI:)2.SUPfuDNA,50L1.6.3PCRPCR,95smin,94lmin,651min,7230,,0.555,30,728min.PCR2%.1.7DGGE)BiRadDcede,PCR.1.7.1,8%,35%%(100%7m0FL40%),.1.7.2PCR,,PCR5L10.1.7.315Ov,604h.,.1.7.4UMAX,(Promega),,.22.12080,RobertsonKuenen[aeraPaPsenasSPPAaligenesfoalis.,Robertson,:neFrette16,.ShWUungPai3Vol.16.,SBR1,,62,DOsmL5TN90%.nf5f7,9().4%91.2%94.6%95.6%97%,,TN30%,5.2.2PCRDGGE(1,,30),16SrDNA38/R518,PcRDGGE1,PCRDGGEld17,15d24,30d13.DGGE,2.ld;2.zsd;3.3odld3od16.%,lPcRFis.1DGGEproofdniPcR.pleS,,sd,.NO3N,,.ldd45.78%,,,,,.15d30dso%,30,,,,.2DGGETable2similatyeoefficienioftheDGGEpro61esld15d30dldld30d45.7816.661oo802.3DGGE7DNANo.l:33,165rDNA(V3),4.DGGE,(OPerationaltaxonounit,OTU),44.GenBank165rDNA,3,2eenassP.,,2,.0ldPCR-DGGE,,,,,,,,.3SBRrIJCneBkle3Themtclly.teheds,iesof6partial165rDNASeqUencesinGeneBankG/%93UneturhterieloneDQ354724(.sP.)(.)PmYU173413(l),SBR1,5,nO,90.4%,91.2%,94.6%,95.6%,97%,;(2)PcRDGGE,,,30,.DGGE,d30d80%,ld30d16.66%,ld15d4578%;(3),26,2,.1,,,.Jl.,2,12(2):292-296Yanzhiy,uYinzh,uX,et.PSS~hofbiolocal~daful,nJ].CJAppEnvllnBIOI,2(X,12(2):292-29634Vol.162RodsonLA,KuenenJG.Co.inedheterotIOphicniconand~bicdscationinmPadotherbacteriaJ].Ant.VanLuWenhoek,l),56:289.2993]BruceER,WayneEL.Simeousdenieaitonwithnitreationinsinechanneldonditches[J].10urnalWPCF,1985,57(4):3(X)08[4]WatanabeY.Simuleousnieationddenieationine~biebioJ.WaterScieneeandTechn010,1992,26(34):511225CpC,BOrdG,PaaudeauM,et.Nitrogenremovalinasubmeedterwithnoeffiuent,nJ].waterScieneeandTechnol,ZJ),42(34):5176ChrisneH,SabineK.Shaultan.uniondeaoninanaebicbihasystemJ.WScienceandTeebnology,1998,37(4):183187[7MunehEV,Pau1L,KellerJ.SimultaneousnitreationanddenieationinbencheSeqlleneingbatch~tors[J].WaterR~h,1996,30(2):2772848,,,J.(),2(X,l(25):758Zh9Guangya,FangBaishan,MinHang,etal.CharacteristiedPhylogenetieansisofastrnofaebiedenitrer[J.oualOfHuaQi,Universsty(Naturalscience),20,l(25):7589HuHK.TSeng5K.Niuonbyeibacterdivemisunderaerobieenviroent[J].ApPIerobiolBioteehnol,2l,55:904roRobertsonLA,VanneilewJ,Torre~,RAM,etd.Simuoniconanddeconin~bicchelneult~OfThihaerapantophApPIJ].E~Meobiol,1988,54(11):2812818[tteL.AobiedeniersiSOlatednlanaltematingactivatedstem[Jj.MsMierobi010Ec010,1997,24:3637012JWingp15,NyukminC,HiangCc.PototialPlieationsofaerobiedeni6yibacteriabioninwastewatertreanentJ.Bio~urceTeehnolo,1999,65:179185AerobicDetersDisPosetheWastewaterofNO3NandtheAnalysisoftheDiversityofcrobiaiSPeeiesSUJunfengl,2,MArangZ,eAoshanshanZ,wANGHon2,uwei02,1uZ(1.SchoolofEn~ndMunicEn~ng,anUni~tyofhitectUreandTeclmoo,an71(X)55,China;2.schoolofMciandEn~nmenEngnng,HinInstituteofTechnology,Harbin15(X0,China:3DepartmentOfMeipalEngineenng,WuhUniversity,Wuhan43(X)72,China)Thepeesseontainedsludaeelimationforeehmentofaerobiedenltriers,bacteriaisolationandpurifieation,theremovofTNare90.4%,91.2%,94.6%,95.6%,97%,bynBf5f7.andtheestablishmentofeontinuousfiowreaetorreactorsurveyedyousindiees.ThePCRDGGEProfilesshowedthatshiftofmierobialdiversityeorrespondtotheeffeetofnitrateremoval.15dandthe30dsimilarity1580%,whiehmierobidiversity15thehighest.CoarationofexeisedDGGEbandsandanalysisofsequeneingresultsthataerobiedenitrifiersandweredominatedbaeteriainseqUeneingbatehreactor.KrW0rds:PCRDGGE;aerobiedenitrifiers;baeterialeonununitystrUeture