好氧反硝化有效微生物群的筛选及特性研究

黄运红1,2,3,冯香玲1,2,龙中儿3,许杨1,2*(1.,330003;2.,330047;3.,330022)[目的]为生物快速脱氮净化水处理提供有用的菌源和技术[方法]通过反硝化培养基定向筛选,从厕所旁土壤中分离获得好氧高效反硝化有效微生物群,并研究其脱氮特性[结果]该有效微生物群命名为OA-IEM,其反硝化速率为143.71mg/(L#d)OA-IEM在以淀粉为有机碳源时,反硝化速率达到了144mg/(L#d),Cu2+Ni2+和Hg2+能抑制OA-IEM的反硝化作用,而Fe3+Zn2+和Mn2+能激活OA-IEM的反硝化作用高浓度硝酸盐也能抑制OA-IEM的反硝化作用,并且随着C/N的增加,OA-IEM的反硝化速率和硝酸盐去除率均有所增加在C/N为25B1的条件下,OA-IEM的硝酸盐去除率达到100%,反硝化速率为184mg/(L#d)[结论]该有效微生物群具有培养基成分简单反硝化速率快等优点,有较好的应用前景定向筛选;有效微生物群;反硝化作用Q936A0517-6611(2007)36-11977-03StudyontheScreeningofAerobicDenitrifyingEffectiveMicroorganismsandTheirCharacteristicsHUANGYun-hongetal(KeyLaboratoryofFoodScience,MinistryofEducation,NanchangUniversity,Nanchang,Jiangxi330003)Abstract[Objective]TheaimofstudywastoprovideusefulbacteriumsourceandtechniqueforfastertreatmentofmicrobedenitrificationandwaterPu-rification.[Method]Throughthedirectionalscreeningofdenitrificationmedium,theaerobicdenitrifyingeffectivemicroorganismswithhighefficiencywasseparatedfromthesoilnearbythewaterclosetanditsdenitrificationcharacteristicswerestudied.[Result]TheeffectivemicroorganismswasnamedasOA-IEM,withthedenitrificationrateof143.71mg/(L#d).Whenthestarchwasusedasorganiccarbonsource,thedenitrificationrateofOA-IEMreached144mg/(L#d).Cu2+Ni2+andHg2+couldinhibitthedenitrificationofOA-IEM,butFe3+,Zn2+andMn2+couldactivatedthedenitrificationofOA-IEM.NitratewithhighconcentrationalsocouldinhibitthedenitrificationofOA-IEM.AndwiththeincrementofC/N,allthedenitrificationrateandremovalrateofnitrateofOA-IEMincreasedslightly,whichcouldreach184mg/(L#d)and100%respectivelywhentheratioofcarbontonitrogenwas25B1.[Con-clusion]Theeffectivemicroorganismshadstrongpointsofsimplemediumcomponentsandfasterdenitrificationrate,etc.,whichhasbetterapplicationfore-groundKeywordsDirectionalscreening;Effectivemicroorganisms;Denitrification长江学者和创新团队发展计划资助(IRT0540)黄运红(1979-),男,江西鄱阳人,在读硕士,讲师,从事环境生物技术方面的教学和研究工作*通讯作者,博士生导师2007-07-31EM(Effectivemicroorganisms),2080,1080[1],,,,[2]EM,[3]200556,,,,,,,,2050,[4-7],,,,,,,11.11.1.1¹20.0g,KNO32.0g,K2HPO40.5g,MgSO4#7H2O0.2g,1000mlº1.1.2¹(5cm),10g100ml250ml,37e200r/min,7d,,º,2h,5ml100ml250ml,37e200r/min7d,5,,4e»100ml250ml,5%(V/V)º,200r/min37e,7d,1.2-[8],[NO3-];N-(1-)-[9]1.2.1600nm,,1.2.2(1),2%,,5%(V/V)/1.1.20º,37e200r/min7d,3(2),JournalofAnhuiAgri.Sci.2007,35(36):11977-11979孙红忠李洪Ni2+Fe3+Zn2+Cu2+Hg2+Mn2+,1mmol/L,,,5%(V/V)/1.1.20º,37e200r/min7d,3(3)KNO31015202530mmol/L,,5%(V/V)/1.1.20º,37e200r/min7d,3(4)(C/N)1020304050g/L,,5%(V/V)/1.1.20º,37e200r/min7d,322.1,,1,,,5.4%,12,,78.0%,OA-IEM12.2,,OA-IEM,NO3-NyNO2-NyNOyNO2yN2,,[10]OA-IEM,(N2),7d,1.288g/L0.280g/L,01.624mg/L,,OA-IEM2.3(OAI-EM)OA-IEM,,2,OA-IEM,,1d,OA-IEMOA-IEM,OA-IEM3d,,1.288g/L0.284g/L,OA-IEM334.670mg/(L#d)22.4OA-IEMKNO3(0.2%),2%3,,,5mg/(L#d),,,144mg/(L#d)OA-IEM,[11]OA-IEM,,OA-IEM:A;B;C;D;E;F;G;H32.5OA-IEM4,,Cu2+Ni2+Hg2+OA-IEM,Hg2+OA-IEM,0,Hg2+OA-IEMFe3+Zn2+Mn2+OA-IEM,Fe3+Zn2+OA-IEM,182mg/(L#d),OA-IEM,OA-IEM2.611978安徽农业科学2007:A;BCu2+;CNi2+;DHg2+;EZn2+;FFe3+;GMn2+4,5,10mmol/L,100%,,OA-IEM,20mmol/L,,OA-IEM,,,OA-IEM52.7(C/N)C/NOA-IEM,6,C/N,OA-IEM,C/N25B1,OA-IEM100%,OA-IEM184mg/(L#d),OA-IEM,OA-IEM3(1),,144mg/(L#d),,(2)OA-IEM,OA-IEM,,1d,,,OA-IEM()6C/N(3)C/NOA-IEM,,OA-IEM144mg/(L#d),Cu2+Ni2+Hg2+OA-IEM,Hg2+OA-IEM,0,Fe3+Zn2+Mn2+OA-IEM,Fe3+Zn2+OA-IEM,182mg/(L#d),OA-IEM,C/NOA-IEMOA-IEM[1]比嘉照夫.EM环境革命[M].日本:综合,1996.[2]李维炯,倪永珍.EM的研究与应用[J].天津畜牧兽医,1995(16):125.[3]张烈,戴俊英.有效微生物群体对玉米植株生长与代谢的影响[J].农业现代化研究,1999,20(6):355-358.[4]MARSHALLRO,DISHBURGERHJ,MACVICARR,etal.StudiesontheeffectofaerationonnitratereductionbypseudomonaddprviesusingN15[J].JBacter-ial,1953,66:254-258.[5]VONMESCHNERK,WUHRMANNK.Beitragzurkenntnisdermicrobiellendenitrif-ication[J].PatholMicrobial,1963,26:579-591.[6]KRULJM.DissimilatorynitrateandnitritereductuinunderaerobicconditionsbyanaerobicallyandanaerobicallygrownAlcaliginessp.andbyactivesludge[J].ApplBacterial,1976,40:245-260.[7]ROBERTSONLA,VANNIELEWJ,TORRESMANSRAM,etal.Simultane-ousnitrificationanddenitrificationinaerobicchemostatculturesofThiosphaerapantotropha[J].ApplEnvironMicrobiol,1988,54:2812-2818.[8]HECHTU,MOHRH.Factorscontrollingnitrateandammoniumaccumulationinmustard(Sinapisalba)seedlings[J].PhysiologiaPlantarum,1990,78:9337-9387.[9]吴国琳.水污染的检测与控制[M].北京:科学出版社,2004:115-117.[10]周丹丹,马放,王弘宇,等.关于好氧反硝化菌筛选方法的研究[J].微生物学报,2004,44(6):837-839.[11]于爱茸,李尤,俞吉安.一株耐氧反硝化细菌的筛选及脱氮特性研究[J].微生物学报,2005,25(3):77-81.1197935卷36期黄运红等好氧反硝化有效微生物群的筛选及特性研究