好氧颗粒污泥胞外多聚物的提取及成分分析

14Vol.1,No.420074ChineseJournalofEnvironmentalEngineeringApr.2007(,310032)EPS,EPS5EPS,,EPS,EPS,35W4min,EPSTOC10513mg/gVSS9615mg/gVSS;(80,60min)EPS,,TOC14216mg/gVSS15312mg/gVSS;EPS,3122518011682163X512A167329108(2007)0420127204ExtractionandcompositionanalysisofEPSforaerobicgranularsludgeZhangLiliJiangLiyingFangFangChenJianmeng(SchoolofBiologicalandEnvironmentalEngieering,ZhejiangUniversityTechnology,Hangzhou310032)AbstractEPSwasthemaincomponentsofmicrobialaggregates,andtheextractionandanalysisofEPSforaerobicgranularsludgewasveryusefulforstudyingthisnovelmicrobialaggregate.Fivemethodsincludingheating,ultrasonication,high2speedcentrifigation,EDTA,andNaOHplusheatingwereemployedforextractingEPSofaerobicgranularsludge,andthekeycomponentsofEPSextractedwereanalyzed.Theexperimentalre2sultsrevealthatthehemogenizationwasanecessarystepforEPSextractionofgranularsludge.Throughcompari2son,theultrasonicationandheatingwerefitforqualitativeanalysisandquantitativemeasurement,respectively.Fortheultrasonication(35W,4min),theTOCinEPSextractionofactivatedsludgeandgranularsludgewas10513mg/gVSSand9615mg/gVSS,respectively.Forheating(80,60min),TOCproductionwas14216mg/gVSSand15312mg/gVSS,respectively.AnalysisforEPSextractedusingdifferentmethodsshowsthattheproteinswerethemostimportantcomponentsinthesludgesamples,andtheratiosofproteintopolysaccharidesingranularsludgeandflocsludgewere31225180and11682163,respectively.Keywordsaerobicgranularsludge;extracellularpolymericsubstances;extraction;analysis:2006-06-09;:2006-09-15:(1978),,,,E2mail:[e-mail](EPS)(Mw10000),,,,[1,2]EPS[1],,[35]EPS,EPS,EPSEPS,,2EPS,,,5EPS,1EPS11.11.1.1(VSS)012gSBR(SBR)EPSSSVSS4,10000r/min(Sigma3218K)15min,,,,40mL(0105mol/LNa3PO4,0115mol/LNaCl,pH=7),45min,40mL1EPSFig.1ProceduresforEPSextractionprocesses1.1.22EPS515EPS1.2(400W)15min,10000G420min,,0122mDNA,DNADNA1.3EPSTOC2LowryDNA,DNATOCmultiN/C3000()22.12,5EPS,,TOCDNATOCEPS,DNA,22EPSTOC3DNADNA2,,TOC8214:22EPSTOC(a.;b.;S.;G.)Fig.2TotalTOCofEPSextractedfromaerobicactivatedsludgeandgranularsludgeusingeachextractionmethod,TOC,,,,,,EPSEPS,,,TOC,EPS,EPS35W4min,EPSTOC10513mg/gVSS9615mg/gVSS,DNADNA5%Jia[6]EPS,(80,60min)EPS,,TOC14216mg/gVSS15312mg/gVSS,DNADNAEDTA,EPS,EDTAEPS,,,TOC,TOC,DNADNA,1615%2418%(3),3EPSDNADNA(a.;b.;c.;d.;e.EDTA)Fig.3RatioofDNAinEPSandDNAincellforeachextractionmethodEPS,EPS,,EPS,,EPS,2.2EPS12EPS2EPS,,11682163,31225180,2EPS,10,EPS,EPS[7],EPS3,:(1)92111EPSTable1EPScomponentsineachgramofactivatedsludgeforeachextractionmethod(mg/gVSS)/+6.42.03.80.81.68-6.82.42.81.62.43(80)+1082.4581.21.86-1123.6552.42.04+732.4382.61.92-684.6323.52.13+1994.8823.02.43-1908.4915.22.09EDTA+291.8112.02.63-272.7133.82.08:+;-2EPSTable2EPScomponentsineachgramofaerobicgranularsludgeforeachextractionmethod(mg/gVSS)/+10.20.92.30.64.43-4.81.21.20.84.00(80)+1203.8381.83.16-744.5232.53.22+852.2253.23.40-291.851.25.80+2158.4684.63.16-8616.3215.54.10EDTA+421.6101.24.20-9.61.42.10.84.57:+;-TOC,,EPS,;,TOC;EDTAEPS,,,TOC,;(2)EPS,EPS,EPS,EPS;(3)EPS,,EPS,EPSTOC10513mg/gVSS9615mg/gVSS;EPSTOC14216mg/gVSS15312mg/gVSS;(4)EPS,3122518011682163EPS[1]LaspidouG.S.,RittmannB.E.Aunifiedtheoryforextra2cellularpolymericsubstances,solublemicrobialproducts,andactiveandinertbiomass.WaterRes.,2002,36:27112720[2]MorganJ.W.,EvisonL.M.,ForsterC.F.Theinternalarchitectureofanaerobicsludgegranules.J.Chem.Techn2ol.Biotechnol.,1991,50:211226[3],,,.SBR.,2004,25(4):7477[4]BeunJ.J.,vanLoosdrechtM.C.M.,HeijnenJ.J.Aero2bicgranulationinasequencingbatchairliftreactor.WaterRes.,2002,36:702712[5]YuL.,LiuQ.S.Causesandcontroloffilamentousgrowthinaerobicgranularsludgesequencingbatchreactors.Bio2technologyAdv.,2006,24:115127[6]JiaX.S.,FurumaiH.,FangH.H.P.Yieldsofbiomassandextracellularpolymersinfouranaerobicsludges.Envi2ron.Technol.,1996,17:283291[7]McSwainB.S.,IrvineR.L.,HausnerM.Compositionanddistributionofextracelluarpolymericsubstancesinaer2obicflocsandgranularsludge.Appl.Environ.Microb.,2005,2:10511057031