好氧曝气时间实时控制实现短程硝化郭建华

ISSN1000-0054CN11-2223/N　()JTsinghuaUniv(Sci&Tech),200949122009,Vol.49,No.12w21　好氧曝气时间实时控制实现短程硝化郭建华2,　彭永臻1,2,　黄惠2,　郑雅楠2,　王中玮2,　孙治荣2(1.,150090;2.,100124):2008-12-22:“”(2006AA06Z319);(50778005);(EDBS07/1-53974092):(1981—),(),,。:,,E-mail:[e-mail]　:为了在处理城市污水中应用好氧曝气实时控制实现短程硝化,研究其机理及操作方式。在3个不同截面形状、不同体积的序批式反应器内,用实际生活污水进行了验证试验。结果表明:利用曝气时间实时控制,在每个周期内氨氧化菌(AOB)降解氨氮质量大于亚硝酸盐氧化菌(NOB)降解亚硝态氮质量;AOB的净增数量大于NOB净增数目。经长期曝气时间实时控制,亚硝化积累率将会逐渐升高。短程硝化启动后,亚硝态氮积累率可维持在80%以上。:短程硝化;生物脱氮;实时控制;氨氧化菌;亚硝酸盐氧化菌:X703.1:A:1000-0054(2009)12-1997-04Partialnitrificationtonitriteusingreal-timeaerationdurationcontrolGUOJianhua1,PENGYongzhen1,2,HUANGHuijun2,ZHENGYanan2,WANGZhongwei2,SUNZhirong2(1.SchoolofMunicipalandEnvironmentalEngineering,HarbinInstituteofTechnology,Harbin150090,China;2.KeyLaboratoryofBeijingforWaterQualityScienceandWaterEnvironmentalRecoveryEngineering,BeijingUniversityofTechnology,Beijing100124,China)Abstract:Thekineticmechanismandoperationforpartialnitrificationusingreal-timeaerationdurationcontrolwasanalyzedformunicipalwastewatertreatment.Thevalidationexperimentwasconductedin3sequencingbatchreactorswithcrosssectionshapesandvariousvolumesusingdomesticwastewaterastheinfluent.Analysisshowsthatmorenitrogenisoxidizedbyammoniaoxidizingbacteria(AOB)thanbynitriteoxidizingbacteria(NOB)withmoregrowthoftheAOBthantheNOBineachcyclewhenusingthismethod.Thenitriteaccumulationincreasesafterlong-termapplicationofreal-timeaerationdurationcontrol.Partialnitrificationtonitritewithnitriteaccumulationratiosofover80%wassuccessfullyachievedusingreal-timecontrolinthethreereactors.Keywords:partialnitrification;biologicalnitrogenremoval;real-timecontrol;ammoniaoxidizingbacteria(AOB);nitriteoxidizingbacteria(NOB),25%,40%,。,,(dissolvedoxygen,DO)、、、pH、[1]。,C、N,[2-4]。,,。,(sequencingbatchreactor,SBR)。1　材料和方法1.1　3、,SBR、SBR-1、SBR-2SBR-3。Multi340i,pH、DO(oxidationreductionpotential,ORP)。:1min,,30min,5min,。SBR-1SBR-2,SBR-33/7。3pH(“”[5])。1。1　/Lθ/℃/dSBR-110—26±130SBR-24126±120226±120326±120SBR-335125±1225±1301.2　3,,[6]。SBR-1(Anoxic/Oxic,A/O);SBR-2;SBR-3。3。1.3　(chemicaloxygendemanding,COD),NH+4-N、NO-2-N、NO-3-N(mixedliquorsuspendedsolid,MLSS)[7],pHWTW340i,ORPWTWpH730,DOWTWMulti340。2　结果与讨论2.1　,pH,,NO-2-N。1SBR-1。1,NH+4-N,pH,“”(am-moniavalley)。DO,“DO”。,pHDO,pHDO,,,NO-2-N。pH“”,pH。1,240minNO-2-NNO-3-N。,NO-2-N。1　2.2　,2。(ammoniaoxidizingbacteria,AOB)(nitriteoxidizingbacteria,NOB)。AOBNH+4-NNO-2-N,NO-2-NNOBNO-3-N,。,,。AOBNOB,NO-2-N。,NH+4-N,NO-2-NNO-3-N,AOBNH+4-NNOBNO-2-N。,AOBNOB[8],AOBNOB。,。,AOBNOB,。NO-2-N:1998()2009,49(12),NO-2-N,AOBNOB。2.3　NO-2-N,,NO-2-N,。,3SBR。2SBR-1(nitriteaccumula-tionratio,NAR),NAR=[d(NO-2-N)+d(NO-3-N)]/d(NO-x-N)。SBR-1A/O,d[DO],,NO-3-N,NO-2-N。SBR-1,d(NO-2-N)d(NO-3-N)6.2425.7mgL-1,NAR20%。NO-2-Nd[DO]。SBR-1,(26℃),,20d。2　SBR-1,NO-2-N。Blackburne[9],(oxygenutilizationrate,OUR)NO-2-N。NO-2-N,10min40mgL-1,NO-2-N。,。,,,NH+4-NNO-2-N。SBR-2,“”,NH+4-N、NO-2-NNO-3-N9.31、10.1314.38mgL-1,NO-2-N。12d,NAR41.3%89.8%。NAR,SBR-22,,2h,NH+4-N,NAR,d(NO-3-N),25dNAR91.8%。,NH+4-N,d(NO-3-N)(3mgL-1),NAR90%。3　SBR-2,SBR-3。4,SBR-3,1NAR57.0%,2NAR78.7%,NAR。SBR-1SBR-2,SBR-3NAR,SBR-33/7,SBR-1SBR-21/2,NH+4-N。NH+4-N(FA)NOB,,NH+4-N60mgL-1,SBR-1SBR-2FA1999,:　1.0mgL-1,SBR-3FA0.8mgL-1。4　SBR-3,FA1mg/LNOB[10],FA[11]。,FA,。,FA。,。Peng[12]UASB+A/O,。,,,。DO。。3　结　论pH“”,“”,。3、SBR,。SBR-1SBR-290%,SBR-380%。NO-2-N,NO-2-N。　(References)[1]ZHUGuibing,PENGYongzhen,LIBaikun,etal.Biologicalremovalofnitrogenfromwastewater[J].RevEnvironContamToxicol,2008,192:159-195.[2]PENGYongzhen,CHENYing,PENGChengyao,etal.Nitriteaccumulationbyaerationcontrolledinsequencingbatchreactorstreatingdomesticwastewater[J].WaterSciTechnol,2004,50:35-43.[3]PENGYongzhen,ZHUGuibing.Biologicalnitrogenremovalwithnitrificationanddenitrificationvianitritepathway[J].ApplMicrobiolBiot,2006,73(1):15-26.[4]YANGQing,PENGYongzhen,LIUXiuhong,etal.Nitrogenremovalvianitritefrommunicipalwastewateratlowtemperaturesusingreal-timecontroltooptimizenitrifyingcommunities[J].EnvironSciTechnol,2007,41(23):8159-8164.[5]GUOJianhua,YANGQing,PENGYongzhen,etal.Biologicalnitrogenremovalwithreal-timecontrolusingstep-feedSBRtechnology[J].EnzymeMicrobTech,2007,40(6):1564-1569.[6]GUOJianhua,PENGYongzhen,WANGShuying,etal.Long-termeffectofdissolvedoxygenonpartialnitrificationperformanceandmicrobialcommunitystructure[J].BioresourTechnol,2009,100(11):2796-2802.[7]APHA.StandardMethodsfortheExaminationofWaterandWastewater[M].20thed.WashingtonDC.USA:AmericanPublicHealthAssociation/AmericanWaterWorksAssociation/WaterEnvironmentFederation,1998.[8]BlackburneR,VadiveluVM,YuanZ,etal.Determinationofgrowthrateandyieldofnitrifyingbacteriabymeasuringcarbondioxideuptakerate[J].WaterEnvironRes,2007,79:2437-2445.[9]BlackburneR,YuanZ,KellerJ.Demonstrationofnitrogenremovalvianitriteinasequencingbatchreactortreatingdomesticwastewater[J].WaterRes,2008,42(8-9):2166-2176.[10]BaeW,BaekS,ChungJ,etal.Optimaloperationalfactorsfornitriteaccumulationinbatchreactors[J].Biodegradation,2002,12:359-366.[11]KimDJ,LeeDI,KellerJ.Effectoftemperatureandfreeammoniaonnitrificationandnit