化感物质抑藻作用研究进展

[e-mail]汪文斌1，孔赟2，郑昱3，朱亮21．3130002．3100583．313000、CO2、。、。、、、、、、。X52A1000－1166201403－0040－08ＲesearchProgressoftheAlgalInhibitionbyAllelochemicals/WANGWen-bin1KONGYun2ZHENGYu3ZHULiang2/1．HuzhouNantaihuEnvironmentalProductionTechnologyCompanyHuzhou313000China2．CollegeofEnvironmentalandresourcesciencesZhejiangUniversityHangzhou310058China3．HuzhouEner-gySupervisingDivisionHuzhou313000ChinaAbstractEffectivecontrolofLakeEutrophicationisthehotresearchtopicinthefieldofenvironmentinrecentdecades．Theallelochemicalsbecauseoftheirabilitiesofhighalgalinhibitionselectivityandenvironmentalharmlessnessareex-pectedtoachieveapplicationinalgalinhibitingfield．Inthispapertheusageofplantallelopathyinhibitingthegrowthofharmfulalgaewassummarizedandtheevaluationmethodsofalgalinhibitioneffectwereanalyzedbasedonthealgalcelldensitychlorophyllcontentultrastructuregrowthmetabolismandantioxidantenzymeactivity．Furthermoretheinhibi-tionmechanismofallelochemicalstotheflos-aquaeandaquaeredtidealgaeweremainlyexpoundedandthegeneexpres-sionofallelochemicalswasproposed．KeywordsAllelochemicalsalgalinhibitioneutrophicationcontrolmechanismCO2、。、CO2。、1－3。Alexandriumtamarense、Chattonellamarina、HeterosigmaakashiwoSkeletonemacostatum4－8。。9－10。7。11－13。04ChinaBiogas2014323、14－15。、。、、。、。131237。。、、、、。41、、2、、34。。3123。、、16－17。。18。2．0～8．0g·L－114。、。。。2、、、、、SephadexLH-20、、。、IＲ、、、、、NMＲMT-3。。5、、、。1。14ChinaBiogas20143232。3。。GC-MSXian192--3-。Greca20GC-MSNMＲEMA-Waridel21UVE-IMSHＲ-ES-IMSNMＲ。1。1、Chlorella、Microcystisaeruginosa12--4-E-3’-124--5-E-3’-α-β-Anabaenaflos-aquae、Scenedesmusquadricandaα-β-γ-Anabaenaflos-aquae、Scenedesmusquadricanda2-EMAMicrocystisaeruginosa、ChlorellaN--1-N--2-、Microcystisaeruginosa、Chlorella4--β-MicrocystisaeruginosaCryptomonasChlamydomonas、Microcystisaeruginosa、Microcystisaeruginosa、、Alexandriumtamarense、Anabaena、Microcystis、Chlorella-4--36-Anabaena、Microcystis、SynechococcusMicrocystisaeruginosaKong225g·L-1EichhorniacrassipeslantadeneAlantadeneB。lantadeneAlantadeneB。Choe23Artemisiaargyi、、Chrysanthemum。GC-MS、、Tellez24ChihuahuaDesertFlou-rensiacernua。。Cantrell25HaplophyllumsieversiFlindersineOscillatoriaperornataSelenastrumcapricornutum1．010．0μmol·L-1。Zhang26ArtemisialavandulaefoliaConyzacanadensisKalimerisindica310d。93．3%2．0×106cell·mL-189．3%4．0×106cell·mL-1。Wu27。24ChinaBiogas20143233--α-。3。3．1对藻细胞膜的破坏。EMASelenastrumcap-ricornutumLiHu28K+Ca2+Mg2+2-EMAChlorellapyrenoidosa、。Sun29Alexandriumtamarense、HeterosigmaakashiwoCochlodini-umpolykrikoides33。20mg·L－130min3h1/218h10mg·L－130min45min18h10mg·L－11h24h1。ABCDEFGHIJK1Sophorolipid33．2对酶活性的影响。、。SODPODSODPOD。SOD。30－31。MDA32－34。Hong3534ChinaBiogas2014323PhragmitescommunisEMAEMA2h1．9124h48hEMA。EMAEMAEMAZhang36MDAO2SODQian37N--2-。2．5mg·L－1SODPODCAT2．473．244．274．0mg·L－1MDAN--2-。N--2-ＲOS。Qian380．2820．08mg·L－1MDA。Hong39———--3--NN-dimethyl-3-amino-methylindole5×1045×105cell·mL－10．52．0mg·L－1MDAＲOSSODCAT4h60hAsAGSH4hGSSG40h。SOD。3．3藻类光合作用与呼吸作用。。。Zhu40MyriophyllumspicatumaⅡⅠ。Hagmann41AIIPSIIQ－APSIIZhang36、a。3．4对藻类基因表达的影响。Shao42———SODCATMADrecAgrpE4mg·L－1psbAmcyBfabZ1mg·L－1D1Shao4344ChinaBiogas2014323D1psbA、prxfabZmcyBrecAgrpE。ATP。DNApsbAmcyBprxfabZDNA、、。a。Qian30N--2-Chlo-rella、psaBpsbC。ShaoWu42MicrocystisaeruginosD1、。、。。4、、。。1。、、。2。。3。、。1SchwarzenbachＲPEscherBIFennerKetal．ThechallengeofmicropollutantsinaquaticsystemsJ．Sci-ence20063131072－1077．2ShannonMABohnPWElimelechMetal．Scienceandtechnologyforwaterpurificationinthecomingdec-adesJ．Nature2008452301－310．3．J．201310855－864．4BallotAPflugmacherSWiegandCetal．Cyanobacte-rialtoxinsinLakeBaringoKenyaJ．Limnologica20033312－9．5HoLHoefelDPalazotSetal．Investigationsintothebiodegradationofmicrocystin－LＲinwastewatersJ．JHazardMater20101801－3628－633．6HoLTangTMonisPTetal．Biodegradationofmul-tiplecyanobacterialmetabolitesindrinkingwatersuppliesJ．Chemosphere201287101149－1154．7LurlingMFaassenEJ．ControllingtoxiccyanobacteriaEffectsofdredgingandphosphorus－bindingclayoncya-nobacteriaandmicrocystinsJ．WaterＲes20124651447－1459．8．J．201372611－2617．9VanHullebuschEDeluchatVChazalPMetal．En-vironmentalimpactoftwosuccessivechemicaltreatmentsinasmallshalloweutrophiedlakePartI．Caseofalu-miniumsulphateJ．EnvironPollut2002120354ChinaBiogas2014323617－626．10VanHullebuschEDeluchatVChazalPMetal．En-vironmentalimpactoftwosuccessivechemicaltreatmentsinasmallshalloweutrophiedlakePartII．Caseofcop-persulfateJ．EnvironPollut20021203627－634．11QinBQYangLYChenFZetal．MechanismandcontroloflakeeutrophicationJ．ChineseSciBullet200651192401－2412．12QuJHFanMH．TheCurrentStateofWaterQualityandTechnologyDevelopmentforWaterPollutionControlinChinaJ．CritＲevEnvironSciTechnol2010406519－560．13TangXQWuMYangWJetal．EcologicalStrategyforEutrophicationControlJ．WaterAirSoilPollut20122232723－737．14XiaoXChenYX．EffectsofTibetanhullessbarleyonbloom－formingcyanobacteriumMicrocystisaeruginosameasuredbydifferentphysiologicalandmorphologicpa-rametersJ．Chemosphere2010811118－1123．15．N3J．201351922－1929．16EverallNCLeesDＲ．Theidentificationandsignifican-ceofchemicalsreleasedfromdecomposingbarleystrawduringreser