环胞苷二棕榈酸酯脂质体粒度分布及微黏度的测定王继波

23220062精细化工FINECHEMICALSVol.23,No.2Feb.2006＊王继波(,　266021):。,,。,37℃,40h0.54μm,1.35μm。。。:;;;:TQ577.77　　:A　　:1003-5214(2006)02-0154-04MeasurementofSizeDistributionandMicroviscosityofDipalmitoylCyclocytidineLiposomeWANGJi-bo(DepartmentofPharmacy,MedicalCollegeofQingdaoUniversity,Qingdao266021,Shandong,China)Abstract:Physicalstabilityofdipalmitoylcyclocytidine(DPCC)liposomewasevaluated.DPCCliposomewaspreparedbyreversephaseevaporationmethod.Comparedwithblankliposome,thesizedistributionandmicroviscosityofDPCCliposomeweremeasured.ResultsshowedthatthemeanparticlediameterofDPCCliposomechangedonly0.52μmafterheatingunder37℃for40h,whilethevalueofblankliposomewas1.35μm.ThemicroviscosityofDPCCliposomewasabout2timesashighasthatofblankliposome.DPCCloadedinliposomeenhancedthephysicalstabilityofliposome.Keywords:dipalmitoylcyclocytidine;liposome;sizedistribution;microviscosity　　,,[1]。,,。,,,,。,,。,,,,。,。,,[2]。,。,,,。,,。,,,、＊:2005-06-04;:2005-09-12:(1962-),,,1988,。,,:0532-83866203;E-mail:[e-mail]。DOI:10.13550/j.jxhg.2006.02.014、。、,,,,,89.14%,1[3],3[4]。,、,。,、[5]。,。,。,40h37℃,,。1　1.1　XZ—804();DL—360();GZ—1();TAⅡ(Coulter);PHS—3B()。LS-50(PE);TB-85();(DPCC)();(PC)();(CHOL)(MERCK);AR。1.2　1.2.1　脂质体的制备,n()∶n()=1∶150mL,3mL。41mg,,,,1∶3,5min,,,,10min,,。1.2.2　环胞苷二棕榈酸酯脂质体及空白脂质体粒度分布的测定1.901×10-3mol/L1mL,w(NaCl)=1%(,0.22μm)100mL,,16。2mL,37℃40h,1mL,。Carstensen:Dn=∑ni=1NiDi/∑ni=1Ni:Dn:;Ni:;Di:。1.2.3　环胞苷二棕榈酸酯脂质体和空白脂质体微黏度的测定。,1,6--1,3,5-(DPH),,,,Perrin(P),(η)。1.901×10-3mol/L1mL,pH7.15(PBS)10mL,10μLc(DPH)=2×10-3mol/L,37℃20min,λex360nm,λem430nm,IVV(0°,0°),IHV(90°,0°),IHH(90°,90°),IVH(0°,90°),Perrin(P)(η):P=IVV-[IHV/IHH]·IVHIVV+[IHV/IHH]·IVHη=2P0.46-P2　2.1　1。·155·2:1　Table1　SizedistributionsofDPCCliposomeandblankliposome/μm/%/μm/%10.42～0.53000.58～0.730020.53～0.672.270.0720.73～0.924.792.1830.67～0.843.830.130.92～1.167.923.4540.84～1.063.854.891.16～1.469.454.8651.06～1.3412.776.801.46～1.8425.9210.5761.34～1.6820.837.651.84～2.3231.7114.0071.68～2.1225.5921.772.32～2.9214.7417.7782.12～2.6718.3627.432.92～3.683.8418.7692.67～3.377.7919.823.68～4.641.1514.87103.37～4.242.939.034.64～5.840.338.11114.24～5.341.162.085.84～7.630.0973.30125.34～6.730.410.257.63～9.270.0571.31136.73～8.480.160.0619.27～11.680.00730.47148.48～10.680.0390.01911.68～14.710.00530.261510.68～13.460.0010.002714.71～18.540.00180.0671613.46～16.9600.002718.54～23.3600.033　　Carstensen:Dn1=2.10μm;Dn2=2.64μm。1,1.06～2.67μm77.6%,1.06～2.67μm63.7%,。:Dn1=2.15μm;Dn2=3.50μm。1.16～2.92μm81.8%,1.16～2.92μm47.2%,。0.54μm,,。1.35μm,,,,。2.2　2。2　Table2　MeasuredfluorescencedataandmicroviscositiesofDPCCliposomeandblankliposomeIHHIHVIVVIVHPη/Pa·s0.0320.0390.1040.0480.2800.3110.0130.0170.0380.0190.2100.1682,,0.311Pa·s,0.168Pa·s,,,,,。,,,[6]。,,,,。,。,。,。,,,,,,,,,,,,。(182)·156·精细化工　FINECHEMICALS　　　　　　　　　　　　　　23　。,,。,,。。,,。:[1]　EtsuoSakai,KazuoYamada,AkiraOhta.Molecularstructureanddispersion-adsorptionmechanismsofcomb-typesuperplasticizersusedinJapan[J].JournalofAdvancedConcreteTechnology,2003,1(1):16-25.[2]　JohnnnPlank.[A].[C].:,2004.13-27.[3]　YFHoust.Newsuperplasticizerfromresearchtoapplications[A].RKDhir,TDDyers.ModernConcreteMaterials:Binders,AdditionsandAdmixture[C].London:ThomasTelford,1999.26-31.[4]　EtsuoSakai,AkiraKawakami,MasakiDaimon.Dispersionmechanismsofcomb-typesuperplasticizerscontaininggraftedpoly(ethyleneoxide)chains[J].Macromol.Symp.2001,175(3):367-376.[5]　Chong-ZhiLi,Nai-QianFeng,Yong-DeLi,etal.Effectsofpolyethyleneoxidechainsontheperformanceofpolycarboxylate-typewater-reducers[J].CementandConcreteResearch,2005,30(8):867-873.[6]　BRongbing,SJian.Synthesisandevaluationofshrinkage-reducingadmixtureforcementitiousmaterials[J].CementandConcreteResarch,2005,35(4):445-448.[7]　　,　,　.[M].:,224-232.[8]　KaoCorp(JP),Processfortheproductionofmethacrylicpolymers[P].EuropeanPatent:EP1209178,2002-05-29.[9]　BDrescher,ABScranton.Synthesisandcharacterizationofpolymericemulsifierscontainingreversiblehydrophobes:poly(methacrylicacid-g-ethyleneglycol[J].Polymer,2001,42(1):49-58[10]　MKinosita,TYuki.Methacrylictypewatersolublepolymerashighrangewaterreducingagentforultrahighstrengthconcrete[J].JapaneseJournalofPolymerScienceandTechnology,1995,52(6):357-363.[11]　KazuoYamada,Ogawa,Shoichi;Hanehara,Shunsuke.Controllingoftheadsorptionanddispersingforceofpolycarboxylatetypesuperplasticizerbysulfateionconcentrationinaqueousphase[J].CementandConcreteResearch.2001,31(3):375-383.[12]　MKinoshita,TNawa,MIida,etal.Effectofchemicalstructureonfluidizingmechanismofconcretesuperplasticizercontainingpolyethyleneoxidegraftchains[A].SixthCANMET/ACIInternationalConferenceonSuperplasticizersandOtherChemicalAdmixturesinConcrete[C].USA:AmericanConcreteInstitute,2000,163-179.[13]　ESakai,JKKang,MDaimon.Actionmechanismsofcomb-typesuperplasticizerscontaininggraftedpolyethyleneoxidechain[A]SixthCANMET/ACIInternationalConferenceonSuperplasticizersandOtherChemicalAdmixturesinConcrete[C].USA:AmericanConcreteInstitute,2000,75-89.[14]　YCTseng,WLWu,HLHuang,etal.Anewcarboxylicacid-basedsuperplasticizerforhigh-performanceconcrete[A].ACISpecialPublicationsSP195[C].USA:Amer