角鲨烯的来源及其制备方法研究进展-王宝琴

1211.2566002.256600：、。：ResearchProgressonSourcesandPreparationMethodofSqualeneWANGBao-qin12RENLi-li11.ShandongProvincialEngineeringandTechnologyResearchCenterforWildPlantResourcesDevelopmentandApplicationofYellowRiverDeltaBinzhouUniversityBinzhou256600ShandongChina2.KeyLaboratoryofFoodSafetyBinzhouUniversityBinzhou256600ShandongChinaAbstract：Theprogressesrelatedwiththesqualeneonsourcesandnaturaldistribution，biologicalactivityandapplication，andcomparisonofpreparationmethodweresummarizedinthispaper.Theprospectsofsqualeneproductionfromtheoil-producingplantsandmicroorganismsresourcesweregeneralized.Thegeneticengineeringandartificialsynthesismethodtoobtainsqualenewillbethenewresearchdirection.Keywords：squalene；aliphaticolefin；terpenoid；preparationmethod；source；biologicalactivityFoodResearchAndDevelopment2015113621DOI10.3969/j.issn.1005-6521.2015.21.050ShandongProvincialNaturalScienceFoundation,ChinaZR2013EML002BZXYL12011970—。Squalene2610151923--2610141822-。6C30H506。。、。11906MitsumaruTsujirnoto[1]。1929Heilbron[2]。1953LangdonRG[3]。LD501800mg/kg5000mg/kg[4]。[5][6]、[7]。。、。、、[7-8]。[9]。[10]。squalenoyldoxorubicinSQ-Dox[11]。193-72℃350℃[12]。250%。15℃0.9[1]。12%[3]。、、。1.16%。51.5%4.4%0.34%、、、、。[13]。201150[14]。[15]。37.74%2.01g/100g。[16]。。33.1、Farnesyl[17]。OwenCO2。50%。CO2、、CO2、。、、CO2CO2。CO2CO2。250bar25MPa333K60℃92%99%[18]。3.23.2.1CN101597204A3.6%98%[19]。CN1849331A。、、。1170℃~320℃13.33Pa~4665.5Pa、、、、、、、、--、-iii2230℃~300℃0.06665Pa~133.3Pa1i、、ii32iii2435364545310%~40%、2%~20%、1%~10%40%~80%[20]。Sugihara。CO230℃、100kg/cm21949.8MPa、CO27mL/min25%supercriticalfluidchromatographySFC50%。CO2268%。97%。Si-gel-SFC81%100%[21]。3.2.2。、、、、。CN1670005C40%。95%[22]。CN101417916CCO2。90%[23]。、-、、。CN1810744AV/V3～50∶11.330Pa～0.133Pa2～580%～90%[24]。CN101190281A0.5%～50%CO2[25]。CO225MPa50℃2h。CO228.83%4.65%1.58%E[26]。HLPCHLPC[27]。Han-PingHeCO250℃、300bar30MPa2.07g/100g0.31g/100g15.3%[13]。KoCO2GC-MSHPLC40℃3000psi20.67MPaCO21499μg/g12.29%、、conjugateddienehydroperoxideCDHP11-diphenyl-2-picryl-hydrazylDPPHCDHPDPPH[28]。3.3。。、、、。。P.BhattacharjeeSaccharomycescerevisiaeTorulasporadelbrueckii41.16237.25μg/g60℃、250bar~255bar25MPa~25.5MPaCO20.2L/minTorulasporadelbrueckii11.12μg/g430.52μg/g-2∶1195[29]。ChangrDNAPseudozymaPseudozymarugulosaPseudozymaaphidis98%4.54g/L5.20g/L340.52mg/L。[30]。Walker。A、A、[31]。CO2、。、。44.1。。。。、、。4.2。。4.3[32]SalanganeSK。。[1]MitsumaruTsujirnoto.Squalene:AHighlyUnsaturatedHydrocarboninSharkLiverOil[J].TheJournalofIndustrialandengineeringChemistry,1920,12(1):63-72[2]IMHeilbron,AThompson.Theunsaponifiablematterfromtheoilsofelasmobranchfish.PartVI.Theconstitutionofsqualeneasde－ducedfromastudyofthedecahydrosqualenes[J].JournaloftheChemicalSociety,1929:883-892[3]LangdonRG,BlochK.Theutilizationofsqualeneinthebiosynthe－sisofcholesterol[J].JBiolChem,1953,200(1):135-144[4]YamaguchiT,NakagawaM,HidakaK,etall.Potentiationbysqua－leneofantitumoreffectof3-[(4-amino-2-methyl-5-pyrimidinyl)methyl]--1-(2-chloroethyl)-nitrosoureainamurinetumorsystem[J].JapaneseJournalofCancerResearch,1985,76(10):1021-1026[5]EleniNaziriandMariaZ.Tsimidou.FormulatedSqualeneforFoodRelatedApplications[J].RecentPatFoodNutrAgric,2013,5(2):83-104[6]RAmarowicz.Squalene:Anaturalantioxidant[J].EuropeanJournalofLipidScienceandTechnology,2009,111(5):411-412[7]KohnoY,EgawaY,ItohS,et.al.KineticStudyofQuenchingReac－tionofSingletOxygenandScavengingReactionofFreeRadicalbySqualeneinn-butanol[J].BiochemBiophysActa,1995,1256(1):52-56[8]ARamirez-torres,CGabas,CBarranquero.Squalene:CurrentKnowledgeandPotentialTherapeuticalUses[M].NewYork:NovaSciencePubInc,2010:1-73[9]AuffrayB.Protectionagainstsingletoxygen,themainactorofsebumsqualeneperoxidationduringsunexposure,usingCommiphoramyrrhaessentialoil[J].InternationalJournalofCosmeticScience,2007,29(1):23-29[10]ChristopherHClegg,RichardRoque,LucyAPerrone,etal.GLA-AF,anEmulsion-FreeVaccineAdjuvantforPandemicInfluenza[J].PLoSOne.20149(2):e88979.PublishedonlineFeb14,2014.doi:10.1371/journal.pone.0088979[11]AndreiMaksimenko,FrancoDosio,JulieMougin,etal.Auniquesqualenoylatedandnonpegylateddoxorubicinnanomedicinewithsystemiclong-circulatingpropertiesandanticanceractivity[J].ProcNatlAcadSciUSA.Jan14,2014111(2):E217-E226.PublishedonlineJan2,2014.doi:10.1073/pnas.1313459110[12]PainterLR,AttreyJS,HubbellHH,etal.Vacuumultravioletopti－calpropertiesofsqualaneandsqualene[J].JournalofApplied196Physics,1984,55(3):756-759[13]HeHP,CorkeH,CaiJG.SupercriticalcarbondioxideextractionofoilandsqualenefromAmarantusgrain[J].JAgricFoodChem,2003,51(27):7921-7925[14]SuarezM,GonzalezP,DominguezR,etal.IdentificationoforganiccompoundsinSanDiegodelosBanosPeloid(PinardelRio,Cuba)[J].JAltemComplementMed,2011Feb17(2):155-65.Dio:10.1089/acm.2009.0587.Epub2011Feb10.[15]PBhattacharjee,VBShukla,RSSinghal,etal.Studiesonfermen－tativeproductionofsqualene[J].WorldJournalofMicrobiologyandBiotechnology,2001,17(8):811-816[16].:,ZL:201010571272.9[P].2013-07-31[17],.、[J].,2010(2):44-46[18]OwenJ.Catchpole,Jan-ChristianvonKamp,andJohnB.Grey.Ex－tractionofSqualenefromSharkLiverOilinaPackedColumnUsingSupercriticalCarbonD