基于长程电子传递的DNA直接电化学检测

快讯第50卷第14期2005年7月DNA熀*(,,210093.*,E-mail:[e-mail])[1].DNA.DNA[2,3].,DNA[4,5].,DNADNA[7~10].DNA,Barton[9~11].DNA,,,DNA[8].DNA[11,12].Long[12]DNA.EBV(Epstein-Barr)DNAPCR,DNA(1).PER5(5’CTTTAGAGGCGAATGGGCGCCA)PER6(5’TCCAGGGCCTTCACTTCGGTCT),EBV,BamH1W(495bp).PCR[13].DNA:10mmol/L(MPA)20mmol/L(MPS)12h.MPSDNA,DNA[14].,5μL5mmol/L(EDC)8mmol/LN-(NHS).,1.25μmol/LPER512h.,5mmol/LEDC8mmol/LNHS1mmol/L12h,.,,Fc-ssDNA-MPA/MPS.2DNAX(XPS).XPSP2pN1s,DNA.S2p,MPAMPS.XPSC1sO1s.3-2Fc-ssDNA-MPA/MPS0.05mol/LpH7.4.3-1,,101mV,+135mV(vs.SCE).+286mV.(20bp),+135mV,[15],DNA.DNA1DNAwww.scichina.com1539第50卷第14期2005年7月快讯2ss-DNA-MPA/MPSXPS30.05mol/LpH7.4PBS1,;2,Fc-ssDNA-MPA/MPS;3,Fc-ssDNA-MPA/MPSEBVPCR2h.100mV·s−1,DNA[9,16,17],DNA[8,16].Fc-ssDNA-MPA/MPSEBVPCR,(3-33-2),364.9%329.2%,DNADNA,,.DNA[16].EBVPCR,181mV,.1mg·mL−1DNA,.,DNA.Anne[15]DNA,[8~10,12,16],DNADNA,DNA,,.,MPSDNA,DNA,495bpDNA,DNA,[11],DNA.8030min.1540www.scichina.com快讯第50卷第14期2005年7月,EBVPCR,(4),DNA.www.scichina.com154140.05mol/LpH7.4PBS1,8030min;2,1EBVPCR.100mV·s−1.,PCR,.(:20325518)(:20275017).1InternationalHumanGenomeSequencingConsortium.Initialse-quencingandanalysisofthehumangenome.Nature,2001,409(6822):860~921[DOI]2PaleckE,FojtaM.DetectingDNAhybridizationanddamage.AnalChem,2001,73(3):74A~83A3Drummol/LondTG,HillMG,BartonJK.ElectrochemicalDNAsensors.NatBiotechnol,2003,21(10):1192~1199[DOI]4KelleySO,BartonJK.ElectrontransferbetweenbasesindoublehelicalDNA.Science,1999,283(5400):375~381[DOI]5GieseB,AmaudrutJ,KohlerAK,etal.DirectobservationofholetransferthroughDNAbyhoppingbetweenadeninebasesandbytunnelling.Nature,2001,412(6844):318~320[DOI]6PascalyM,YooJ,BartonJK.DNAmediatedchargetransport:CharacterizationofaDNAradicallocalizedatanartificialnucleicacidbase.JAmChemSoc,2002,124(31):9083~9092[DOI]7OkamotoA,TanakaK,SaitoI.RationaldesignofaDNAwirepossessinganextremelyhighholetransportability.JAmChemSoc,2003,125(17):5066~50718KelleySO,BoonEM,BartonJK,etal.Single-basemismatchdetectionbasedonchargetransductionthroughDNA.NucleicAcidsRes,1999,27(24):4830~4837[DOI]9KelleySO,JacksonNM,HillMG,etal.Long-rangeelectrontransferthroughDNAfilms.AngewChemIntEd,1999,38(7):941~945[DOI]10BoonEM,CeresDM,Drummol/LondTG,etal.Mutationdetec-tionbyelectrocatalysisatDNA-modifiedelectrodes.NatBio-technol,2000,18(10):1096~1100[DOI]11FanCH,PlaxcoKW,HeegerAJ.Electrochemicalinterrogationofconformationalchangesasareagentlessmethodforthese-quence-specificdetectionofDNA.ProcNatlAcadSciUSA,2003,100(16):9134~9137[DOI]12LongYT,LiCZ,SutherlandTC,etal.Acomparisonofelec-tron-transferratesofferrocenoyl-linkedDNA.JAmChemSoc,2003,125(29):8724~8725[DOI]13FaresF,HabibM,VerniolC,etal.InsituamplificationoftheEp-stein-Barrvirusgenomeincellsuspensions.JVirolMethods,1998,71(2):211~218[DOI]14LevickyR,HerneTM,TarlovMJ,etal.Usingself-assemblytocontrolthestructureofDNAmonolayersongold:aneutronre-flectivitystudy.JAmChemSoc,1998,120(38):9787~9792[DOI]15AnneA,BouchardonA,MoirouxJ.3'-ferrocene-labeledoligonu-cleotidechainsend-tetheredtogoldelectrodesurfaces:novelmodelsystemsforexploringflexibilityofshortDNAusingcyclicvoltammol/Letry.JAmChemSoc,2003,125(5):1112~1113[DOI]16WongELS,GoodingJJ.Electronicdetectionoftargetnucleicacidsbya2,6-disulfonicacidanthraquinoneintercalator.AnalChem,2003,75(15):3845~3852[DOI]17HartwichG,CaruanaDJ,deLumley-WoodyearT,etal.Electro-chemicalstudyofelectrontransportthroughthinDNAfilms.JAmChemSoc,1999,121(46):10803~10812[DOI](2005-04-30,2005-06-06)