体内药物分析技术的研究现状及展望

*汤建林综述,周世文审校(第三军医大学新桥医院临床药理基地,重庆400037):体内;药物分析;技术:R917:A:16718348(2008)08087604(biopharmarmaceuticalanalysis),,,,,,,,1,,,,gng,,,,,,,,,,,(SPE)(SPME)(MD),1.130,,,,,,,,,:(1),,,,,,Peng,(HPLC)3,,,,Haginaka[1](S)(S),,,C18(2),,,WatersOasisHLB,,()[2~4],Mallet[5]96,2mg,750L,25L,30,,Yang[6],WatersOasisHLB96,300L70L(HPLCMS),0.1ng/mL,0.1~100ng/mL1.2SPE,1989Pawliszyn,,,SPE,SPME,,,,,SPME,[7]SPME,,,,(GCMS)(LCMS),1.3,,,(perfusate),(extracellularfluid,ECF),,,,,,,876重庆医学2008年4月第37卷第8期*:(CSTC2006BB5080):[phone];Email:[e-mail],(dialysate),ECF,,,;,;,,2090Wang[8],26.3%,3!10-8mol/L,,,,Clment[9],HPLCUV,,,1L,3g/mL1.5g/mLClment,,(),,12.3%17.9%,5.5%17.7%,MDHPLCMDHPCEMDGCMDMSMDHPLC/MsMDBiosensor,2,,,,(chromatography)2080,,,,,(ultraperformanceliquidchromatography,UPLC)[10,11],(monolithicchromatography)[12,13],(hydrophilicinteractionchromatography,HILIC)[14,15]UPLC2m,1/3~1/6,50mm,1000barHPLC,UPLCYu[16]UPLCMS/MS5,HPLCMS/MS,2,,,HPLC,UPLC3,10,2,50.08~9.85ng/mL,4,r20.991~0.998,UPLC,,,,UPLC,,,(1m),(),,,Huang[13],C18,,3.2mL/min,1.2mL/min,,,,5min,10min,1,,,,,,ESIMS(),,,Song[17]HILICMS/MS5OH0.05mL96,0.5mL,0.1mL0.4mL,10LBetasil(50mm!3.0mm,5m),,2min95∀5∀0.173.5∀26.5∀0.1,1.5mL/min,2.7min,,2.5ng/mL,,,,,3#∃,,,[18],(UV)(PDA)(EC)(ELSD),,(CLND)(MS)(NMR)(CLND),HPLC,,pmoL[8],LCMS,5,LCMS/MS100[19],[20](LC/APCI/MS),14,124h,,11g,APCI/MS14,877重庆医学2008年4月第37卷第8期,,,;,24h,3.0h11.0h;APCI/MS3O1,LCMS,,,(NMR),,SPENMR,NMR,NMR,Connor[21]HNMR,,,,1,1,NMR,NMR,,,,;,;;,,MS,MS,,LCMS/MSLCNMRLCNMRMS,,,:[1]HaginakaJ,SanbeH.Uniformsizedmolecularlyimprintedpolymersfor2arylpropionicacidderivativesselectivelymodifiedwithhydrophilicexternallayerandtheirapplicationstodirectseruminjectionanalysis[J].AnalChem,2000,72(21):5206.[2],,,.[J].,2006,34(7):995.[3],,,.[J].,2006,26(2):184.[4],,,.TFO[J].,2002,31(11):1029.[5]MalletCR,LuZ,FiskR,etal.Performanceofanultralowelutionvolume96wellplate:drugdiscoveryanddevelopmentapplications[J].RapidCommunMassSpectrom,2003,17:163.[6]YangAY,SunL,MussonDG,etal.DeterminationofM+4stableisotopelabeledcortisoneandcortisolinhumanplasmabyLutionsolidphaseextractionandliquidchromatography/tandemmassspectrometry[J].RapidCommunMassSpectrom,2006,20:233.[7]MusteataFM,PawliszynJ.Bioanalyticalapplicationsofsolidphasemicroextraction[J].TrendsAnalChem,2007,26(1):36.[8]WangZP,ZhangZJ,FuZF,etal.Flowinjectionchemiluminescencedetectionforstudyingproteinbindingofterbutalinesulfatewithonlinemicrodialysissampling[J].JPharmBiomAnal,2003,33:765.[9]ClmentR,MalinovskyJM,CorrePL,etal.Spinalbiopharmaceuticsofbupivacaineandlidocainebymicrodialysisaftertheirsimultaneousadministrationinrabbits[J].InteJPharm,2000,203:227.[10]AlDirbashiOY,AboulEneinHY,JacobM,etal.UPLCMS/MSdeterminationofdoxazosineinhumanplasma[J].AnalBioanalChem,2006,385(8):1439.[11]WrenSAC,TchelitcheffP.Useofultraperformanceliquidchromatographyinpharmaceuticaldevelopment[J].JChromatogrA,2006,1119(30):140.[12]BorgesV,YangE,DunnJ,etal.Highthroughputliquidchromatographytandemmassspectrometrydeterminationofbupropionanditsmetabolitesinhuman,mouseandratplasmausingamonolithiccolumn[J].JChromatogrBAnalTechnolBiomedLifeSci,2004,804(25):277.[13]HuangMQ,MaoY,JemalM,etal.Increasedproductivityinquantitativebioanalysisusingamonolithiccolumncoupledwithhighflowdirectinjectionliquidchromatography/tandemmassspectrometry[J].RapidCommunMassSpectrom,2006,20:1709.[14]EerkesA,ShouWZ,NaidongW.Liquid/liquidextractionusing96wellplateformatinconjunctionwithhydrophilicinteractionliquidchromatographytandemmassspectrometrymethodfortheanalysisoffluconazoleinhumanplasma[J].JPharmBiomedAnal,2003,31:917.[15]XueYJ,LiuJ,UngerS.A96wellsinglepotliquidliquidextraction,hydrophilicinteractionliquidchromatographymassspectrometrymethodforthedeterminationofmuraglitazarinhumanplasma[J].JPharmBiomedAnal,2006,41(7):979.[16]YuK,LittleD,PlumbR,etal.HighthroughputquantificationforadrugmixtureinratplasmaacomparisonofUltraPerformanceTMliquidchromatography/tandemmassspectrometrywithhighperformanceliquidchromatography/tandemmassspectrometry[J].RapidCommunMassSpectrom,2006,20:544.[17]SongQ,NaidongW.Analysisofomeprazoleand5OHomeprazoleinhumanplasmausinghydrophilicinteractionchromatographywithtandemmassspectrometry(HILICMS/MS)Eliminatingevaporationandreconstitutionstepsin96wellliquid/liquidextraction[J].JChromatogrBAnalTechnolBiomedLifeSci,2006,830:135.[18]KohHL,YauWP,OngPS,etal.Currenttrendsinmod878重庆医学2008年4月第37卷第8期ernpharmacyeuticalanalysisfordrugdiscovery[J].DrugDiscovtoday,2003,8:889.[19]XuRN,FanL,RieserMJ,etal.RecentadvancesinhighthroughputquantitativebioanalysisbyLCMS/MS[J].JPharmBiomAnal,2007,44:342.[20],,,.[J].,2003,12(5):360.(:20071008)杨海平综述,张才全审校(重庆医科大学附属第一医院普外科400016):胃癌;预后;进展:R735.2:A:16718348(2008)08087903,,,,11.1,,40,5,5,,1.2,[1]1.3,(EGC)5,[2],TNM,,[3],%&∋(575.65%58.73%28.01%18.42%,∋(%&,pTNM1.4,:,[4]524%,17%,11%,10%5(P0.01)[5]557,5%,558,4%,,,,,1.5,4cm,[6]4cm568.4%,4cm5(P0.05)4~10cm534.8%;10cm52.1%,,,1.6,53[7]3(N1N2N3)16,D1D2D3Ichikura[7]pN,N010~15;N120;30,pNKikuchi[8]pN330,Nitti[9],25%1.7ABC3:ABC,,,,D2590%,,,,,30%,D2D3,,De879重庆医学2008年4月第37