Chapter 5 GLC

TransmittalNo.96-E1(9/96)FormFDA2905a(6/92)PesticideAnalyticalManualVol.ISECTION500500–1TableofContentspagedate501:GeneralInformation501A:Principles501-11/94501B:EquipmentforGLC501-11/94GasChromatographicComponents501-11/94OtherApparatus501-21/94ReagentsandGases501-31/94501C:ResidueMethodologyforGLCDetermination501-31/94Cleanup501-41/94ReagentBlanks501-51/94ChoiceofSolvent501-61/94501D:InjectionTechniques501-61/94ManualInjection501-61/94Autoinjectors501-71/94501E:ReferenceStandards501-81/94References501-81/94Chapter1RegulatoryOperationsChapter2GeneralAnalyticalOperationsandInformationChapter3MulticlassMRMsChapter5GLCChapter4SelectiveMRMsChapter6HPLCTransmittalNo.96-E1(9/96)FormFDA2905a(6/92)500–2SECTION500PesticideAnalyticalManualVol.Ipagedate502:Columns502A:Introduction502-11/94ColumnSpecifications502-11/94ColumnParameters502-21/94502B:PackedColumns502-61/94ComponentsofPackedColumns502-61/94PreparationofPackedColumns502-81/94InstallationofPackedColumns502-91/94ConditioningofPackedColumns502-101/94RejuvenationofPackedColumns502-101/94CriteriaforAcceptablePackedColumns502-111/94RecommendedOperatingProcedures502-121/94forPackedColumns502C:OpenTubularCapillaryColumns502-131/94ColumnDescription502-141/94InjectionontoCapillaryColumns502-161/94CapillaryColumnSystems502-161/94InstallationandConditioningof502-201/94CapillaryColumnsRejuvenationofCapillaryColumns502-211/94RecommendedOperatingProcedurefor502-221/94WideBoreColumns(Isothermal)ApparatusandReagents502-221/94SystemStartupandInspection502-251/94References502-251/94503:Detectors503A:Introduction503-11/94DefinitionsofDetectorCharacteristics503-11/94503B:ElectronCaptureDetector503-21/94Principles503-31/94Design503-31/94ApparatusandReagents503-31/94DetectorCharacteristics503-41/94OtherInfluencesonDetectorPerformance503-71/94RecommendedOperatingProcedures503-81/94503C:FlamePhotometricDetector503-91/94Principles503-91/94Design503-91/94ApparatusandReagents503-101/94DetectorCharacteristics503-101/94OtherInfluencesonDetectorPerformance503-111/94RecommendedOperatingProcedures503-121/94Troubleshooting503-131/94503D:ElectrolyticConductivityDetector503-141/94Principles503-141/94TransmittalNo.96-E1(9/96)FormFDA2905a(6/92)PesticideAnalyticalManualVol.ISECTION500500–3pagedateDesign503-141/94ElCD-X503-161/94Principles503-161/94ApparatusandReagents503-161/94DetectorCharacteristics503-171/94OtherInfluencesonDetectorPerformance503-191/94RecommendedOperatingProcedures503-211/94SystemSuitabilityTest503-211/94Troubleshooting503-221/94ElCD-N503-231/94Principles503-231/94ApparatusandReagents503-231/94DetectorCharacteristics503-231/94OtherInfluencesonDetectorPerformance503-241/94RecommendedOperatingProcedures503-251/94SystemSuitabilityTest503-251/94Troubleshooting503-251/94GeneralPrecautionsforElCDs503-261/94503E:Nitrogen/PhosphorusDetector503-271/94Principles503-271/94Design503-271/94ApparatusandReagents503-281/94DetectorCharacteristics503-281/94OtherInfluencesonDetectorPerformance503-301/94RecommendedOperatingProcedures503-311/94References503-321/94504:Quantitation504A:Introduction504-11/94504B:ManualQuantitation504-21/94MeasurementofPeakHeight504-21/94MeasurementofAreabyTriangulation504-21/94504C:ElectronicIntegration504-31/94504D:SpecialConsiderationsforComplex504-61/94ChromatogramsBHC504-61/94Chlordane504-81/94PCBs504-101/94Toxaphene504-141/94References504-151/94505:BibliographyGeneralTexts505-11/94Inlets505-11/94Columns505-11/94Detectors505-11/94TransmittalNo.96-E1(9/96)FormFDA2905a(6/92)500–4SECTION500PesticideAnalyticalManualVol.IpagedateFigures502-aPolysiloxaneStationaryPhases502-21/94502-bGLCColumnParameters502-41/94502-cFerrulesforConnectingGlassandMetal502-91/94502-dCapillaryColumnCross-Section502-151/94502-eInletAdaptersforCapillaryColumns502-171/94502-fVanDeemterCurves502-191/94502-gCapillaryColumnInletSystem502-231/94503-aTwoECDetectorDesigns503-31/94503-bReproducibility503-51/94503-cDynamicResponseRanges503-61/94503-dLinearResponseRange503-61/94503-eEffectofDetectorTemperature503-71/94503-fEffectofCarrierGasFlowRate503-71/94503-gSingle-FlameFPD503-91/94503-hDual-FlameFPD503-101/94503-iBlockDiagramoftheElCD503-151/94503-jElCDReactorandConductivityCell503-151/94503-kN/PDetectorComponents503-281/94503-lN/PDetectorConfigurations503-291/94504-aManualPeakMeasurement504-21/94504-bTriangulationofPeakonSlopingBaseline504-31/94504-cTechnicalChlordane504-81/94504-dChlordane,Heptachlor,HeptachlorEpoxide504-91/94504-ePCBsinChinookSalmon504-131/94504-fToxaphene504-141/94Tables502-a:CommonGLCLiquidPhasesUsedinPesticide502-31/94ResidueDetermination502-b:OperatingConditionsforPackedColumns502-131/94504-a:EffectsofChangingElectronicIntegrator504-61/94Settings504-b:ResponseofTwoDetectorstoFour504-71/94BHCIsomers504-c:WeightPercentFactorsforIndividualGas504-121/94ChromatographicPeaksinAroclorReferenceStandardsPesticideAnalyticalManualVol.ISECTION501501–1TransmittalNo.94-1(1/94)FormFDA2905a(6/92)501:GENERALINFORMATIONMultiresiduemethodologybydefinitionrequiresdeterminativestepscapableofs