碱性蛋白酶产生菌的筛选

:1001-3717(2006)01-0070-033,,(,266109):59,SHL-4SHL-6SHL-7SHL-10SHL-8SHL-11SHL-12SHL-18SHL-20,SHL-4SHL-6SHL-7SHL-10;,SHL-4,0.5%1.5%:;;:Q939.97:AScerrningRningofAlkalineProteaseProducingStrainsLIShu2wen,LUXiu2hua,SHANGHai2li(LifeScienceCollege,LAC,Qingdao266109,China)Abstract:Ninestrainsproducingalkalineproteasewerescreenedoutfromfivesoilsamples,namelySHL-46710811121820,SHL-46710ofwhichwithhighproteaseactivity.Throughanor2thogonaltest,theproducingproteaseculturemediumoftheSHL-4strainwasconfirmedpreliminarily,namely0.5%ofpeptone,1.5%ofbeefextract.Keywords:alkalineprotease;screening;strain,,[1,2],,,,,[3]4,11.11.1.1,51.1.2:1.0%,0.5%,0.5%,1.6%,pH10.0;:;:1.0%,0.5%,0.5%,1.6%,1.0%,pH10.0;:,25.0,100.0r/min;:2.0%,0.5%,0.5%,KH2PO40.1%,MgSO47H2O0.02%,pH10.0,25.0,100.0r/min;:2.0%,2.0%,pH10.01.2[4]1.2.11)10.0g,90.0ml,10-1,1.0ml9.0ml,,10-2,10-310-7()23(1):7072,2006JournalofLaiyangAgriculturalCollege(NaturalScience)3:2006-01-05:(1978-),,,,,:,,2)10-510-610-70.2ml,3,253)2d3d4d,,1.2.2,,,,1.2.3SHL-41)2,25,100r/min,24h,2)2.0%,25,100r/min,12h24h36h48h,31.2.4SHL-41)1.2.32)L9(34),2%,24h,25,100r/min1.2.5[5],10.0ul,2524h22.11d,,2d3d4d,4520,SHL-1SHL-2SHL-20,22.2,,209,,:SHL-4SHL-6SHL-7SHL-10,SHL-8SHL-11SHL-12SHL-18SHL-20,SHL-4SHL-6SHL-7SHL-10,SHL-42.3SHL-411,24h36h,12h48h,,24h112h24h36h48h(mm)18.017.020.023.024.024.023.024.023.022.020.021.02.4SHL-422,,,,RRR+,SHL-4A2B1,1.5%,0.5%3,,9,SHL-4SHL-6SHL-7SHL-10,SHL-4,24h,1.5%,0.5%,25,100r/min,,50,,[7],171,:2L9(34)[6]A(%)B(%)A+B+(mm)11(1.0)1(0.5)1121.022.021.021(1.0)2(1.0)2220.018.019.031(1.0)3(1.5)3321.020.020.042(1.5)1(0.5)2322.022.024.052(1.5)2(1.0)3123.022.024.062(1.5)3(1.5)1224.021.023.073(2.0)1(0.5)3222.023.024.083(2.0)2(1.0)1315.019.016.093(2.0)3(1.5)2124.020.021.0T1183201182198T2205176190194T3184195200180R22251818,:[1],.[J].,2004,30(4):93-96.[2]HorikoshiK.Alkaliphiles-fromanindustrialpointofview[J].FEMSMicrobiolRev,1996,18:259-270.[3],,,.[J].,2004,21(4):20-25.[4],,.[M].2,:,19,107-112.[5],,,.YS-9412-130[J].,2000,21(4):1-5.[6],.[M].:,2001,25-30.[7],,.[J].,2002,22(5):16-20.2006118,,,,,,,,,,,,,27()23