您好,欢迎访问三七文档
当前位置:首页 > 行业资料 > 国内外标准规范 > DB61∕T 409-2007 牛奶制品中硝酸盐的测定-酶法
DB61if*~il§DB611T409-20072007-09-03~1b2007-09-03~nmDB61rr409-2007*;fiffft~i¥JIlfB~A:J.J~~4ttIlfHk0*~fft~~$A~~~~~®WA~~~~~M~W*~Po*~fft~¥.~:~$A~~~OO~~WA~~~~~~o**iffft~~A:@:~r*,$f*f~,Il*a~,~KffWo*fiffft*1Hx!t1ti0DB61ff409-20071$'i!~:$:,j:iJ;fiffllj£Y!W~m~j£tfWj$IJrPn9=J~FilWttL?;ltB'~7J1;L:$:*;f;fi~mr·tfWi$UMl9=J{if!4lWttL*jj:;;l~j£02tJ.tH'M1s1mJt1tf~F)iIJxf'tq~f8~~iii1ii:$:fiJ;fttfJ'31mrm}JX;;I;J:$:,fiFfitfJ~~J:ofL:MJtEl~JJf8i31Jf.lX1'1j:,;ltMiJFiJifil'Ji8f~e.k;qi(::f1~H6llJji9:~V-J)l1J;fl~n~:i)j~mJ+lT:$:f;f;{i,~rm,!Z!EJJtlH,@:$:f;j;fti6:r&t:h'i~I¥-J~·1J~)ftL~~Uf1~J:fji3:J!!';.Xfij:tfJiilI#f~:$:0fLM:IfffBWJtfJi31H:lXf!i:,;ItiiifJi~:$:jjjJ:Wf:$:fiJ;f1LDIN10476tfWj$U~n9=J.fi~MHll&~{if!4~1tRtfJlYl~IDF97AtfWj#liJ&~Lmf)}9=J{if!4!'fittLf8jl)l~j£NADP ~tw~)tH:~J1~n~1lt=*~tr~(trBIWII)NADPH ~.~5.*~~~&i(~~~.IWU)±b~,Rt.!nilliM..H71+:iIfi:tjFAD ~~I.J)J(?:R~-1·i'j~J::,gIllif.;j:f,R,v,-iI!ii!'NR l'f.j~11t1.rVL~HllfU f!iij1~00~iJ;ill.,:fr25°Cf8f;J;fi~14.FTI}*~rp1UtfJ~.J&.IIImoltfJW~oAIJ&Yt~{!CwmttfJ*~NRM~1tR+NADPH+H__......\niJi~~:tk+NADP+H~ONADPHtfJ,\Sl,JjtIE.fi~&HRtfJ,tt;,:liit~•NADPHtfJ~j.jtf±340nm~1'I:··~·tfJUl1YtJJt1J1~:(~05iltfflJ5.1~~tiHP;'&:5.2NADP5.3FAD5.4NR5.5Hel5.6NaGH5.7KGB5.8:mJ~)JDB611T409-20075.9.!lEt~1l1.ttlflK1Fe(CN)n•3H205.10i1U§it$ZS01•7H205.111:!!Rf-1tff.ljiij~ilt*IJ1i6.1ft~*ft(5mL1mL50-200j.lL,10-50j.lL)6.2btEilll:7't1£1.Oem6.3~:9Ht7't7'tJ}tit,TIJ~~-~,1£340nrnW!~JEo6.4lJT1f;;rz:tK,JL1£10j.lrn06.5fJlJ'I),tJl6.6i~H:'\l~\~;ft,ft~H~J!j[:,:~y~=*,.JllZ~;W~1;f0:}Y1~j7~7.1ilt1fIJ!ciM7.1.1~~!i;IPRf;ffi.0.68gQ;t(~mT-80mlrp,mHCIifflr'l1pH~7.8,J:l:jm:~*JEm3S:100mL7.1.2NADPI1t;-&mImLQ;t(~~iP~~§f(flO.5mgNADP,tj:mLfllO.OlmgFADot!P);}NADPyWo7.1.3 f~IiElinR~lfih~d¥~:m0.8mL4U#fj~MJmlt~,~Plg#fj~Mil£iU!lH~;i~4i?Jo7.1.4HeI:2moIlLo7.1.5NaOH:2moi/Lo7.1.6KOH:2moi/Lo7.1.7Carrez;~Rf7.1.8Carrez-Ii~~85mmolILCarrez-Im~:~Jtx3.60gI(4Fe(CN)6·3H20,1.mA100mL*~.I./.m~lf.c7. 1.8.1Carrez-IIm~250mmollLCarrez-IIm~:l$l1X7.20gZ,S01•7thO,:t.JI1AlOOmL£~*mmo11fii1fJHB1,ACarrez-Im~fllCarrez-II-m~,J't?t¥M6..J~iJl]Carrezmm.o7.2ilt*$iMi7.2.1 ~i*ltt2rnL(g)fMW,1JnA98rnLm:~JJ(,m~!;(JnJEJ~j't.j~~¥~107Hrf,rt;7J\.~Et6-J,7.2.21tiIi*l1X5g~~{i}fi¥,:I1JJffiilx'I/:I'nli*nrT'n]fT~¥frp,11I1A50mL~l~:ffi}~.I./.,:(£r~;J(mrp{~1iflSminJFi,IttUH'tW(20-2S·C),hilA6mLCarrez~ff.m.,mNaOHiJ6lpH8,tt~~w:*birp,mM~*JE?f.l-J100mL.¥M';J,][{IMfDOmin,i1~IE:(;j:ij.~~~iTh)LmLJf:L),J:l:jr),£r~~~Y!~o7.3*1t:j~IJ(t£~1ilHT)7.3.1 i.i~s~1~~lttbtBilll,11XiXEI1Al.OOOmLNADPr:1t¥1X,O.lOOmLNAD1:1t:~,2.000mL£~JJ(,¥tttJlI3min~l{V)*7't&m(AI)'nhOA0.050mLM~Mil£.ltIiiH~j-¥4m,1~¥t~tr&rw.%JiX:(A~~40min),i!tltt~lB't&1.il(A2),~A(AI-A2)'i't't,i120minJFiffHtHti.9;lttll&ft&1.ilA307.3.2~~t[~l:l::Ellil,f1XtXIJIlA1.000mLNADPI.{t~,O.lOOmLfl~d~~,1.900mLiI!:~JJ.¥bttJ3minJRi9;.EfXPllYt&111(AI)'flfbuA0.050mL1i¢jWR.I&~f!ifHrl;l'f4PJ,f}Y1hlttJxrv.Jcnli:(A~~40min),i.'1;lt'Z!l},Z)DB61/T409-20077'tll-ffi.(A2),tfMIJ&7'tll-ffi.&=(At-A2)If,ri120minff:if!}~tH~w;1IXi11B't&ffiA3;*~.~M~~e~IJ&7't&m&,m~M~7't&m~.~~e~7't&ffi~m~~~0~(I):6A=[(Al-A2)IU~-2X(A2-As)M.]-[(Ai-fu)x(A2-A~).....................(0if!m~IJ&7'tJlm&@:;kT0.100,:1:J~~~illl~U¥HiJfJ~tii:W:0*1#lJlj'~ff~$~~eIff.~nNADPlft~1.OOOmLI1.OOOmLf+.®~~-O.100mL:iJ~*2.000mLj1.900mLitE:fIJ,R~iW&IN.jG1£Ii@m,~J3min,~lIXi1lt7'tllfgAl,mJff:i;IJpA-F9u~J.OO::iffl1~1ib:EJi~JtlUHf~0.050mLI¥fUIl,;tq;&Rit!qf:iG1£m(~J40min),JL~pi9;lIXflffn;flJ~ei¥J1l&7't!l:f1IA,{±20minm~~w;llXll.&7'tllf1IA3,11~ittflI:if'fI}5Nt.O.050mL-8it.ftUi\!i0;i:.\;(2)it~¥.tI9::VjXMWc-----------XLIAX[··..································(2)£XdXV2X10009NJf~1ib:~:i:it1t~1Jit~1Jr-05:\:3.050X62.0c=--------XL1AXf£X1.OOXO.100X10001.891----XL1AXf•iter:c~~~PW!ii1)gH8j:R&:giL•MWVIV]fdtL1A~~~p!I!ii1J9i~J~HJ\J.OO::!to~~~M1J*~Mid::62.0g/molbtBliller~)W1J\~~i¥J;~,1*lR,Jlf!.1i'O!f:mLEl3li\f-fftlj~i¥JffMl~~Rq,~l,W:fR,Jlf!.f:;7:;!lt::mLf-fM~HlflJ#ff!ff1tr~!:tBlill~J't;fitL!fl.{:;7::fi:emNADPHflJr~:1t*~:fr340nm6.3[Ixmmorlxem'l]~7't&ffi§:tIIffIrr!f~().\~~MM1J9it£#H&Ji-1-f;j\m~#AIWM1/tm:X~!10.2Mfi.t¥JINJ+J1Il3IDB61/T409-200710.2.1~:bOivtH::mH5i~Ij&JilZ1**t:p010.2.2:;ij!~JHtl:itt1ifE-j:&Ji17.f-FJhmH5i&JiI7.t~MLA2mB~Q,11110.050ml!'t'1JJttt£H5iJJIlJjIj;f£rij!~nE.g.H5it:p,&liY.tiliWJFi(IOmin),••~OO~~~(Ar~)*••~~,JiIZ~~~~~~1*~••~ft&m~.~~.~.%Nm~JJIl010.2.3pgfffm~w~~~~~,~~~~~~MW:ittfr~.,~fE#~m.t:p~~~~mf~.~2mH~li\:~iI7!i1:bIlAl:t5;f1;t:pA~m¥&1:3f+,~1.f~mt+n~+f,T\mNADPTfn1?il+~nmWlmfirn~&::m.:m;Jj(LOOOrnL-2.000mLl.OOOmLO.lOOmL1.900rnL1.000mLO.100mL1.900mL1.000mL0.050mLO.050rnL1.9{)OmL¥~*n,fi~1lrJ&JilZj£{£&JiI7.~,~J3min,f¥~ij;!#dt0w;Itt.jtt~m[Al]'~Hf17.3;f£r~ul~1f¥*11::rU;f£r?!J1UW.2X.1AI!J~+tJIff-.1Arr,}JX100[%}......................................(3).1AfME•4DB61ff409-20071li1:1RA(i!i*!/.'ti1li1:1R)11:f:$lgr1.~~M.~~m~~~~~~Wtt,mM~~~~£*~~~~••~.2.~~1ttl~f~~.i{q-l¥~.~:iflj{*~f;f)l~q,JlIZ-ff:r'2·C~8'Cj!!jcffiuJiX,~NADP_t:1t~.~Jtj.~IDlItIJ:,~d-¥•.4!WtE2aC~8'CDJ~J:£~~!tJ3o~i5(XijfjflJ:li~'fllmJlj!!jcffiIJoI3.».~.{q-l~~m~~~~~~~~~m.IIIiIIi5lI!f
本文标题:DB61∕T 409-2007 牛奶制品中硝酸盐的测定-酶法
链接地址:https://www.777doc.com/doc-8108483 .html