您好,欢迎访问三七文档
当前位置:首页 > 行业资料 > 国内外标准规范 > DB61∕T 412-2007 食品中甘油的测定--酶法
DB611T412--20072007-09-03~~2007-09-03~nffi1DB61rr412-2007I1*flFft9=tffJ~t:?kA1v:6't*'i-'ti~t~0*~ft~9=t~A~~~~MDiliA~•••~~.ili#~Do*~ft~~.m:9=t~A~~~~M~iliA~•••~~o*~ft~~A:~~a*,~*1~,~lM~,*lfWo*flFft~ttij\Zt;1fl0DB61rr412-20071m:m*t;)';mm!IE7rn&tit~UIE~n~r:pit7IDRJ~:Ii:0::$:;fi-;mi(fmT~tWtr:ptrmnl'J~WIE02ni!m:'1'1i]Iilt)i:itt:.,JrYI1::tfljcr:pFfJ~~Jmi:1*;f;)';(f:Efn5\mITOT!Y;)J*;f~;ff1tf.J~~ofLH:tl:BWJRJJlJ:ft~1tt-,JtMIJRpJf1JFfJ.~.(~~m~*FfJ~~)~.u~~~~mT*~ff1,~ITO,a~*~::$:~ff1~~~~~&~~~~:S:itJttmJ2,:®X1tFFfJ:!iifr~*Jrt:M~1'iB~J:jfni§ImX1ILJti,lfF;fi~*mmT**~;ff10 0IFU77(2001)--ii~¥tr:pitillJRJ~UJEEBC9.33-ru¥mt:p1=t¥1±lRJ~~JEOWii7t)3*~~IUE:)t3.1*tF-ltlflfiMi*~PJlt:*~,iiiljt,M,!l!tWi,~*~,~~~3.2f1~!:j!Tlfilll&i!OK tt~U]~1fATP .=::fW.fwttr~fl\ADP wttr--fW.f~PK p;jifr!lIMl~~PEPfW.f~m-~iJtp;jIi!WPEP-CHA~Wm-IW5:\;jAjIi!WfjIlfz..'fJ(1i1;:L-LDHL-!¥Lrutn~Jm~1fNADH B-1W~-M:Rn~Ilt--~tnw~NADmlliM:~Tl~1lt-f~tr!!t:U lfiifr&OO~ffi-~1i/.,25°CFfJ*h~ff1~1!1:-~'TIj:mintUFfJMiifri9JitlHtJJX:1\.ImolFfJ4mJ!lLAfl&Jt~11II C4m&fFfJrtll1l4mi:t1lfIit;iIbfrit¥dJ~IWFfJ11E1t~'1iJf&ATP~!!t:ft)'.l3-~~itnJJn~,ATP$t:1tJgADP;&Ji\1J:tJ'.l:OKGlycerol+ATP..L-glycerol-3-phosphate+ADPDB6lIT412-2007PKADP+PEP--.....ATP+Pyruvate.fJg~l:l1il3lHEL-~Ul~H!~Jljt~!liHI]ftm-FPIl&:NADHif~J]L-?JLMW~,NADH:nk:$tft7-]NAD;&~:itA:L-LDHPyruvate+NADH+H'----.~L-Iactate+NAD'5.1ttitMttitM5.2NADH,ATP,PEP-CHA5.3MgS04'7H025.4p;j~MiJi.5.5L-~fin~llStlt.5.6tt~fQiJi.~;¥4?I.I5.7·HCI5.8NaOH5.9KOH5.10SlH!Ullf-tt!JI~Fe(CN)dH105.11i1t~l$ZnS04'7H205.12£m)J5.13tBPIif:ilHfli8f.lit1fIJ:&6iift6.1~Hi+ft(5mL,1mL,50-200ItLlO-501t06.2tt~J1ll::J'(;r£l.Ocm6.3~9Ht:J'(;:J'(;Jiit,PJ3'£;&:*,:(£340nm;~IJ;iE6.4jff:fl~!t,1H£10JAm6.5~IL'*Jl6.6ilIIL'~'~~,~;l+ftlC:gji!i~,f§~~~7~WlfJ!I7ilJ7.1lit1fIJDcitl]7.1.1ttjAMttjUD~~/pHi:#Fo.66git~mcit~~mT90mUj(rp,}tjNaOHiJW'j'ipIHlI3:?:7.4.JE~3:?:lOOmL7.1.2$m.1iUi/PHitr..J5~Ur:t4?l.l:!+XlImLtt~rutFn.Kml~.iFP~ez,mMlf.NADHt~7mg:ATP~\l22mg:PEP-ClIM\lllmg;nUAlmollLf¥JMgSO,•7HO~4mL2DB61fT412-20077.1.3p;jIlilD~.,L-~LDI~Mti\.~i.!¥4tlJ:~J-Hftj,ImL1!?li7.kif.Hfl1j!600up;jllilmU~iI!Ff~5J&550uL-¥LmHrnn~ltlio7.1.4tt)m~.~j.!¥4tlJ:ffllmLm:~*mM85UAtlit¥rtl~l'jo7.1.5HCl:2mollLa7.1.6NaOH:2mollLa7.1.7KOH:2mol/La7.1.8Carrez~;.5ij7.1.8.1Carrez--Im~!85mmollLCarrez-Im~:;f$lf:{3.60gK4Fe(CN)6'3H20,hui\100mL;t€1$17.kmM07.1.8.2Carrez-IIm~250mmol/LCaITez-IIm~:~lf:{7.20gZNS04·7H20,hui\100ml,m:tm7.kmfU~oli{ljf!ffla1,~lthui\Carrez-Im~;fUCarrez-I1-m~,JE7H~~~P)'JCarrezm~o7.2 lit*~itIJ.7.2.17C@~;i@~rrmKOHi~~25ml~¥tpHm3I7r~8,hu1tHl7.k:Wti)fif,11!m1.000~1.800mL~~m:1ffft~no7.2.2~¥;!R~;t*~~mKOBi~~25m1~HpH{fi~7~8,i1~lli¥tt¥.'!!~n,If:{m~aJjQ£r~f~jz~1~):jfffft~lLj7.2.31!Ii*~Il*I!Ii**$~~I7.2.3.1~¥Ef;~!kW~1S~If:{25gffrfng:~Wf~:/:)J)JIi:ti1l1*:fI.lTlt!lf*t+n~,m50mLl.I!J§).kff6fi:mNf,hfli\10mLCarrez~m~,JE~m~,w~tt~,~~~x~~m¥.'!!W~1S~,If:{M~ffl~fft.o7.2.3.2~Jf;f.f.M~~A)JIi:mCarreziJ\ifIJiiJJl#.~f?f:~J:j:JAtl~B)JIi:,25mL~:i:.i1i1~rtH.fJidff~,hfli\lOmLCarrez~'!;,);!j,n;3000r/min!!'JiG'10min,~~~JtlT:f&~mo7.2.3.3~*;f.f.IW1~M§,JVJ!1HH~v.k1rtJm:25gf¥~J:j:JAtlH5'Wi(1rtJm:t!!Jl'i&fE~5'WiAtlmfU¥Jjt~-.t),ft~)§~?ttl5:±l~[j'JVJ,:#f~w)Jli:$t'~3I$fj!*liJ:j:J*;lJI]JK~50mL.i;J.#t15minffr))§i1M,t~7.k:trtJj{FnJ1:l10mLCarrezm;l/~}ft~,••ItX.¥j~mffft}1jmo7.3 ~~IJtv!ll(1ti~1ilHT),7.3.1i9::£s~;f~~{:~bt1SJlIl,*ljz:IJfli\1.000mL~~¥~;fflW,2.00OmLjl~/.k,0.010mLp;j1il~~It,L-¥U~Vli~Jl#.i1.I!fUH'f.W, mir]i6min)Ri*.$:Il,&7'tm1!L(AI)'~JFihlli\0.010mLttntJ~.~/-¥W,ffl¥!iir&@(*~/;j5~-10min),:lL~P~It)?Il,&7'tJ.tff[(Al),~BIl,&7'tmrn&=(AI-A2)o7.3.2*~~:.Ef?,*ix:IJuA1.00OmLtlTIlt¥!i;fIlW,0.100mLt+r~MftH~,1.900mL:!IlJ~;.k,0.010mL]:Ajli]l'Pi~~,L-¥Ul~H]~n#'~It~~7-¥w,1!iir'if(6minffTi*ItX.~,&7'tNf!L(AI)'~fr1hfli\0.010mLitntJ~~;t112ff:Zl,(Aft]5~lOmin),ttpl#ifxQ}}.7'tN{1lCAl)'t+rThQ,&7'tNm7$:=(A1-A2)fl'M,o~!J}IJ~Jl~t+rThAAtlQ&7'tHHI'I7$:,rmJFiJiH+~~Q,&7't[~{l'l:7$:~:t:YAq&J'tNfllkIJJl;l.1~1\IJ~A(I):3DB611T412-20076A=(AJ-A2)1l',lI,-(AJ-A2)'!i!s............................................(l)liiJ5I¥JI!&JtJl1!&@::krO.100,/Jfj~~~IJlii~~I¥J~J!L'=o.jm*I!&JtJl1!&6AtE334l!J..340nm·F~~~ag1!itfir1.000!l)(;::{E365nmr~~~agmiftr0.500,~n~~.~ff~.M~trMI¥J~Jl~.otoAIttgIDll¥JmMsmmtff~t1t~~,~l'ine~~ff£m.:m~7j(~1fnJ~.1'i'L-.!JL~JrnJmitl'i~~~1.0002.0000.0101.000o.1001.9000.010ne~,][~iW&@%~&@J5,~'.J5--7min,~J{;{I!&JtJl1!AIffijJ5toAr9U~J9t:trM.ru,Hf~0.0100.010ne*u,1,lj&@:itf1TjG~J5(~g5--10min),.n:ep~J{;{;ff£~~sagI!&JtLt1!A2~!J*:t£15minJ5&@Mt*d~Jl:,JjJlJ:t£2minagI'Eij~l*Jm~~J{;{it~,][~.tlt-f1I/fW~1to8it.*j1iH~5t(2)it1HRJl:V,XMWC=::---------XL1AX/....................................................(2)eXdXVzX1000trM~:l:it1l:J1HIi~lrF05t3.020X92.1c,---------XLlAX/eX1.00XO.100X10002.781-----XLlAX/eCttilfl(g/L#rfltlGfIl)CtJiIfI=---------X100(g/lOOg)·········•·••••···..················..···....(3)4DB61ff412-2007~TWJIJ-1-,@1y$iJ!JI!&J'tItffI~,f-Frt1~~iJ!J~ItltHf~'-f.t£O.04g/LO.4g/Lz.r~.Atf:C~tlL~m4mJD.i:iJ!J~It:gtt~/L~&it~~MWtt~$JD.i::JI::92.1g/molVItt'@.IIlltfiY!tliAfif~iJ!J,~f/.$:fT-,ljiffL!;mLV28.liJ\f-FitIJ~iJ!Jf-FrI1tfif~iJ!J,~\f/.$:~,F~&itfif~R''1~~fa~dtt'@.llJLiJ!JJ't~!i,lj
本文标题:DB61∕T 412-2007 食品中甘油的测定--酶法
链接地址:https://www.777doc.com/doc-8108577 .html