Pin1、β-连接素和细胞周期素D1在老年肺癌组织中的表达

华中科技大学硕士学位论文Pin1、β-连接素和细胞周期素D1在老年肺癌组织中的表达及临床意义姓名：赵俊申请学位级别：硕士专业：老年医学指导教师：刘建200904011Pin1-D1Pin1-(-catenin)D1cyclinD192209220Pin1-catenincyclinD11Pin1-catenincyclinD1P0.012Pin1-catenincyclinD1P0.05Pin1P=0.032P=0.041-cateninP=0.0123Pin1-cateninP=0.000Pin1clinD1P=0.157Pin1-catenincyclinD1Pin1Pin1Pin1-catenincyclinD12TheexpressionsofPin1-cateninandcyclinD1inelderlylungcarcinomasandtheirsignificanceCandidate:JunZhaoTutor:Prof.JianLiuABSTRACTObjectiveToinvestigatetheexpressionsandcorrelationsofPin1-cateninandcyclinD1inelderlylungcarcinomas.MethodsTheexpressionsofPin1-cateninandcyclinD1wereexaminedinthespecimensof92elderlylungcarcinomasand10normallungtissuesbyimmunohistochemistryandex-ploredtherelationshipbetweentheexpressionlevelsandclinicopathologicalfactors.Results(1)ItwasfoundthatsignificantdifferencesofexpressionlevelsofPin1-cateninandcyclinD1exsitbetweenthelungcancergroupandthecontrolgroup,theexpressionslevelofPin1-cateninandcyclinD1inthelungcancergroupwerehigherthanthatinthecontrolgroup(P0.01).(2)InlungcarcinomastheexpressionsofPin1-cateninandcyclinD1correlatedwithtumordifferentiation(P0.05).ThepositiveexpressionrateandintensityofPin1correlatedwithtumorstage(P=0.032)andlymphnodepositivedisease(P=0.041).Theexpressionof-catenincorrelatedwithlymphnodepositivedisease(P=0.012).(3)HighexpressionlevelsofPin1correlatedwithaberrant-cateninexpression(P=0.000)butdidnotshowacorrelationwithcyclinD1(P=0.157).ConclusionInelderlylungcarcinomasthepositiveexpressionofPin1causesabnormalaccu-3mulationof-cateninandactivesitstargetgene,however,thistargetgenewasnotcyclinD1.ThedetectionofPin1expressionhadsomeclinicalsignificanceinestimatingprognosisofelderlypatientwithlungcarcinomas.Keywordslungcarcinoma;proteinPin1;protein-catenin;proteincyclinD1;immunohistochemistry_____4Pin1-D1(Peptidyl-prolylcis/transisomerasePPIasePin1)[1]-(-catenin)Wnt-cateninTcf/lefc-myccyclinD1[2]Pin1Pin1-catenin[3]-catenincyclinD1Pin1-catenincyclinD1Pin1SPPin1-catenincyclinD1Pin1-catenincyclinD15120041200769220925735608570114338382826I26II37III27IV2UICC2002TNM593334584547HE22.1ONLYMPUSLEICARM-2135TN-1008HaierPH10µl200µl1000µl2.2HE40%610%--20062.31Pin11100OncogeneResearch2-catenin1100Zymed3cyclinD11100Zymed4SPGBIA5%BSABIgGC5DABGBIABDABC6APESGBI7344.1130min220g40ml360ml98%312h604APES492%APESAPES30s5364.2124µm34µm(4/)SPSS13.0HE84602h530min4.3HE16025min235min41%30s15min(50)5min55min674.4Pin11602hIII15min100%90%75%,52PBS25min30.3%H2O210minPBS35min4PH6.00.192-9415530minPBS35min63720min7PBSPin150µl3790min8PBS5min×39PBS3725minPBS5min×3101hPBS5min×3911PBS100µlDAB122min0.1%-10s15min1375%90%100%5minIII15min144.5-catenin“PBS-catenind110050µl3790min”6cyclinD1“PBScyclinD1110050µl3790min”6Pin1:1PBS50µl5Pin1-catenincyclinD14005100500≤2502650151742≥753012326SPSS13.0Pin1-catenincyclinD12χPin1-catenincyclinD1SpearmanP0.05101Pin1-catenincyclinD192Pin14650.00%10.00%2/20P=0.001-catenin5660.87%40.00%5/20P=0.004cyclinD16065.22%25.00%4/20P=0.0002Pin1-catenincyclinD12.143Pin127,62.79%38Pin11539.47%11Pin1436.36%P0.05Pin1-catenin72.09%52.63%45.45%P0.05-catenincyclinD158.14%71.05%72.73%P0.05cyclinD1(1)1Pin1,-catenincyclinD1Pin1-catenincyclinD1+-P+-P+-P4327163112251838152320182711114756839246460.07056360.01860320.0482.2Pin184.62%35.71%36.84%P0.05Pin111-catenin84.62%46.43%55.26%P0.05-catenincyclinD188.46%50.00%60.53%P0.05cyclinD1cyclinD122Pin1,-catenincyclinD1Pin1-catenincyclinD1+-P+-P+-P2622422423328101813151414381424211723159246260.00056360.01160320.0092.3Pin166.10%21.21%P0.05Pin1-catenin69.49%45.45%P0.05-catenincyclinD159.32%75.76%P0.05cyclinD13123Pin1,-catenincyclinD1Pin1-catenincyclinD1+-P+-P+-P593920411835243372615182589246460.00056360.02360320.2002.4IPin126.92%II48.65%III+IV72.41%Pin1P0.05Pin1I-catenin73.08%II54.05%III+IV58.62%P0.05-cateninIcyclinD157.69%II70.27%III+IV65.52%P0.05cyclinD144Pin1,-catenincyclinD1Pin1-catenincyclinD1+-P+-P+-PI267191971511II37181920172611III+IV29218171219109249460.00356360.30060320.5872.5Pin143.86%60.00%P0.05Pin113-catenin54.39%71.43%P0.05-catenincyclinD157.89%77.14%P0.05cyclinD155Pin1,-catenincyclinD1Pin1-catenincyclinD1+-P+-P+-P5725323126332435211425102789246460.13356360.10460320.0602.660-69Pin153.13%7042.86%P0.05Pin160-69-catenin59.38%7064.29%P0.05-catenin60-69cyclinD162.50%7071.43%P0.05cyclinD166Pin1,-catenincyclinD1Pin1-catenincyclinD1+-P+-P+-P60--69643430382640247028121618102089246460.36556360.65760320.408142.7Pin144.12%53.45%P0.05Pin1-catenin50.00%67.24%P0.05-catenincyclinD152.94%72.41%P0.05cyclinD177Pin1,-catenincyclinD1Pin1-catenincyclinD1+-P+-P+-P34151917171816583127391942169246460.38856360.10260320.0582.8Pin144.68%55.56%P0.05Pin1-catenin63.83%57.78%P0.05-catenincyclinD163.83%66.67%P0.05cyclinD188Pin1,-catenincyclinD1Pin1-catenincyclinD1+-P+-P+-P47212630173017452520261930159246460.29756360.55260320.775153Pin1-catenincyclinD1SpearmanPin1-cateninr=0.301P=0.0049Pin1cyclinD1r=0.144P=0.171109Pin1-catenin-cateninPin1012345601143211146436232163212311681174212531351413961337914917231289210Pin1cyclinD1cyclinD1Pin10123456033511185235232121233212174