喷雾压力、药液流量和工作风速对生防微生物在不同作物上定殖的影

©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(JiangsuJ.ofAgr.Sci.),2008,24(5):6406441,1,2,2,1,2,1,1(1.,210014;2.,210014):200823228:863(2006AA10A211);(BE2006354):(19752),,,,(Tel)025284391002;(E2mail)[e-mail]::(SF2193)215MPa;,4(2100L/m)SF2193:;;;;:S491:A:100024440(2008)0520640205InfluenceofDifferentOperatingPressuresandSprayVolumesandWindVelocitiesonBiocontrolStrainsColonizationonCropsLIUYou2zhou1,CHENZhi2yi1,FUXi2min2,WUPing2,LUOChu2ping1,GONGYan2,LIUYong2feng1,NIEYa2feng1(11InstituteofPlantProtection,JiangsuAcademyofAgriculturalSciences,Nanjing210014,China;21KeyLaboratoryofPlantProtectionMachineryEn2gineering,MinistryofAgriculture,Nanjing210014,China)Abstract:Theinfluenceofcolonizationonseveralcropsunderdifferentoperatingpressureandsprayvolumeandwindvelocityofbiocontrolstrainswerestudied.Theresultsshowedthatstretcherpowersprayer,powermist2sprayerandgal2luspowersprayerhadnonegativeinfluenceonbioactivityofbiocontrolstrains(includingBacillussubtilis,Bacillusthuring2iensis,BeauveriabassianaandfungiSF2193).Thestrainscouldcolonizeonplantsurface.Populationofthesestrainscouldbeincreasedgreatlywhenoperatingpressureofpowersprayerwashigherthan215MPaandsprayvolumereached2100L/m.ThepopulationofB.subtilisonricesurfacewaspositivelyrelatedtocontroleffectagainstricesheatblight.DifferentwindvelocityhadnoinfluenceoncolonizationonlettuceleafofB.bassianaandcontroleffectonSF2193againstAlternan2theraphiloxeroides.Keywords:operatingpressure;sprayvolume;windvelocity;biocontrolstrain;colonization,[1],,046©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[2],,,[3],[(B.sub2tilis)(B.bassiana)(B.thuringiensis)]SF2193[4,5](),SF2193(A.philoxeroidesGriseb),(Nimbyaalternantherae);,,;,SF2193,,,1111(215%+1ml11010)2000IU/lSF21933WKY403WFB218ATF900TF900,015MPa,2155L/m11230,(Rhizoctoniasolani),PDA(200g1020g1720g1000ml)YPGA(5g5g5g20g1000ml)LB(10g5g10gNaCl20g1000mlpH710)(4010g1010g110g015g015g2010g1000ml)SF2193PSA(200g20g20g1000ml)1133WKY40110MPa115MPa215MPa315MPa415MPa,5,2,142000IU/l500ml(150),10,100ml2h[6]3,10d110,,3,1cm251021143WFB218A3(31100m/s),1(0193L/m)2(1155L/m)4(2100L/m)3,2,14113146:©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(150)20d,=/100%=(1-/)100%116SF21933WFB218A,2(33m/s),4(29m/s)2,2,5m220g3,100ml2h3SF2193PDA,287d7mm,PDA(100ml3),28130r/min3d,10000r/min15min,SF2193200ml(110),10d[7]:5,20,=()/()100[8]2211,2,(1)110MPa115MPa215MPa315MPa415MPa,1cm2210105410105910105310106110107415MPa110MPa50115MPa25215MPa11(1A)1cm2310106510106110107510107810107415MPa110MPa27115MPa16215MPa8(1b)10d110MPa115MPa215MPa315MPa415MPa,1cm2310104910104114105210105110106415MPa110MPa33,115MPa11,215MPa7(1A)1cm2414105514105115106610106110107415MPa110MPa23115MPa19215MPa7(1b)2123,10d(2)4(2100L/m)1cm22106(2a),1cm2810107(2b);2(1155L/m);110d4(2100L/m),10d1cm2310105,1cm2112107213(1)315MPa415MPa4(2100L/m)42462008245©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(%)415MPaOperatingpressureofstretcherpowersprayer415MPa73149a2100L/mSprayvol2umeofpowermist2sprayer2100L/m71104abGalluspowerspray70177ab315MPaOperatingpressureofstretcherpowersprayer315MPa69169abc1155L/mSprayvol2umeofpowermist2sprayer1155L/m65138bcd215MPaOperatingpressureofstretcherpowersprayer215MPa64190bcd115MPaOperatingpressureofstretcherpowersprayer115MPa62164cd()Wateringpot(CK)61128d0193L/mSprayvol2umeofpowermist2sprayer0193L/m60183d110MPaOperatingpressureofstretcherpowersprayer110MPa60156d0105Differentlettersinthesamecolumnpresentnosignificantdifferenceatthe0105level.2Fig.2Effectsofcolonizationofdifferentsprayvolumeofpowermist2sprayeronB.subtilisandB.thuringiensisonricesurface,1(0193L/m)1100MPa3,315MPa415MPa4(2100L/m)4,1(0193L/m)1100MPa3(1cm2210105),214SF219342,1cm2110106;346:©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(2):SF2193,2SF2193Table2Colonizationofdifferentwindvelocityofpowermist2sprayeronlettuceleafandcontroleffectonstrainSF2193againstA.philoxeroidesTreatment1cm2PopulationofB.bassianaonlettuceleafin1cm2SF2193ControleffectonstrainSF2193againstA.philoxeroides(%)Windvelocity(33m/s)1106a70aWindvelocity(29m/s)2106a69aCK2106a73a0105Differentlettersinthesamecolumnpresentnosignificantdifferenceatthe0105level.3,,,,,,()(SF2193)215MPa,;2100L/m,:[1],,.[J].,2003,29(3):324.[2],,,.(Bacillussubtilis)B2903[J].,1993,25(1):69272.[3],,,.[J].,2006(3):40242.[4],,,.B2916[J].,1997,13(2):75278.[5],,,.B2916[J].,2002,18(1):45246.[6].[M].:,1998:50.[7],,,.SF2193[J].,2007,(2):61263.[8],,.SF2193[J].,2007,23(4):3532357.4462008245