繁殖季节长江口斑尾刺虾虎鱼的形态_生殖力与卵巢组织学特征

323201008MarineFisheriesVo.l32,No.3Aug.,2010:1004-2490(2010)03-0257-07:2010-03-23:(2008M01),,:(1977-),,,,,Emai:[e-mail]:.Emai:[e-mail]1,2,1,2,1,2,2,1,1(1.,,200090;2.,201316):,(TL)(SL)(HL)(SL)(SL)(BD)(BW)(SW)(BW)(SL):TL=0.7796SL+1.7939;HL=0.2999SL+7.288;SL=0.7832BD+7.3249;BW=0.8184SW-4.9796;BW=0.0629SL2.3315(F)6328~59878egg,(SL)(SW)(OW)F=94.972SL2-4832.2SL+833435;F=83.823SW+14084;F=507.54OW+13190(F)(GSI):F=2375.54+525.67SL-31.44SW+199.43OW+261.74GSI(FL)8831egg/mm,(FW)15754egg/g1.220.24mmIVIV,IV,,;,,:;;;;;:S917:AMorphologicalcharacter,individualfecundityandovaryhistologyofAcanthogobiusommaturusinYangtzeRiverestuaryFENGGuangPeng1,2,ZHANGLongZhen1,ZHUANGPing1,CHENJie2,ZhangTao1,HouJunLi1(1.EastChinaSeaFisheriesResearchInstitute,ChineseAcademyofFisherySciences,KeyandOpenLaboratoryofMarineandEstuarineFisheriesResourcesandEcology,MinistryofAgriculture,Shanghai200090,China;2.CollegeofFisheriesandLifeScience,ShanghaiOceanUniversity,Shanghai201316,China)Abstract:Morphologicalcharacter,individualfecundityandovaryhistologyofatotalnumberof86individualsofAcanthogobiusommaturuscatchedfromYangtzeestuarywerestudied.Thefollowingregression2010equationswhichshowedclosecorrelationoftotallengthandstandardlength,headlengthandstandardlength,standardlengthandbodydepth,bodyweightandnetbodyweigh,tbodyweightandstandardlengthwereobtained:TL=0.7796SL+1.7939;HL=0.2999SL+7.288;SL=0.7832BD+7.3249;BW=0.8184SW-4.9796;BW=0.0629SL2.3315.Theindividualabsolutefecundity(F)variedfrom6328to59878eggs.Thecorrelationrelationshipbetweenindividualabsolutefecundity(F)andstandardlength,netbodyweigh,tovaryweightwereasfollows:F=94.972SL2-4832.2SL+833435;F=83.823SW+14084;F=507.54OW+13190.Thebodylengthrelativefecundity(FL)was8831eggs/mmandbodyweightrelativefecundity(FL)was15754eggs/g.Theeggdiameterwas1.220.24mm.IntheovariesatstageIV,theoocytesweremostlyatphaseIV.TheoilballbegantoappearintheoocytesatthelatephaseIV.Nucleusshowedexcursionanddistortion.Zonaradiatawasclearatthisphase.Intheovariesatstage,theoocytesshoweddissociation.Thetwolayersoffollicularmembranewhichwereoutsidetheplasmamembranefellof.fKeywords:Acanthogobiusommaturus;individualfecundity;biology;ovary;oocyte;histology,92702211,[1],95106307,13.9%,66,241[2](AcanthogobiusommaturusRichardson),,(Gobiidae)(Acanthogobius)[2],,,,,,,150~200g,450~500g[2];,,[3-4],,70,[5-10],,11.120084~6,1,86ind,21.7~43.6cm,101.7~505.5g1.2,1mm,(totallength,TL)(standardlength,SL)(bodydepth,BD)(headlength,HL)(snoutlength,SnL)(eyediameter,ED)(interorbitalwidth,IW)(caudalpedunclelength,CL)(caudalpeduncledepth,CD)9,(bodyweigh,tBW),0.1g,(somaticweigh,tSW),,(ovaryweigh,tOW),,!~0.040g(0.001g),50~100egg,Olympus,,ImageProPlus5.1,1960egg,5~6m,H.E.,,Olympus[11][12][13]2583:1.3STATISTICA6.0Excel2003,:(K)=/3∀100(HSI)=/∀100(GSI)=/∀100(F)=/∀(FL)=F/(FW)=F/22.10.550.09,3.70.1,1,(TL)(SL)(HL)(SL)(SL)(BD)(BW)(SW)(BW)(SL):TL=0.7796SL+1.7939(R2=0.9389);HL=0.2999SL+7.288(R2=0.5884);SL=0.7832BD+7.3249(R2=0.9389);BW=0.8184SW-4.9796(R2=0.974);BW=0.0629SL2.3315(R2=0.9042),,1Tab.1SomeratiocharactersofA.ommaturusRatiocharactersMinimumvalueMaximumvalueAveragevalueStandarddeviation/SL/BD0.730.990.890.06/SL/HL0.400.480.440.03/HL/SnL2.593.962.810.25/HL/CL0.971.511.170.16/HL/ED5.807.626.950.46/HL/IW3.835.935.010.68/CL/CD3.084.894.100.542.221.7~43.6cm,29.36.4mm;101.7~505.5g,181.7105.6g;71.5~432.8g,141.691.5g;5.5~101.5g,25.222.1g(F)6328~59878egg,246076328eggF10000~40000egg,91.18%(1)(FL)16~168egg/mm,8831egg/mmFL40~120egg/mm,88.57%(2)(FW)18~285egg/g,15754eggFW100~250egg/g,80.00%(3)25920101Fig.1FrequencydistributionofindividualabsolutefecundityinA.ommaturus2Fig.2FrequencydistributionofbodylengthrelativefecundityinA.ommaturus3Fig.3FrequencydistributionofbodyweightrelativefecundityinA.ommaturus2.32.3.1生殖力与生物学指标的相关分析FFLFW3(SL)(SW)(OW)(GSI)(K)515,2,FK,FLSLSWK,FWOWK6,9FK,,,F,,F;FL,,FL;FWSLSW,,,FW,,FW2Tab.2CorrelationcoefficientsbetweenindividualfecundityandbiologicalcharacterindexesIndexSLSWOWGSIKF0.60*0.59*0.85*0.44*-0.24FL0.180.210.73*0.65*0.09FW-0.51*-0.56*0.160.71*0.29:*5%Note:*meanssignificantdiferenceat5%2.3.2生殖力与生物学指标的回归关系5(F)(SL)(SW)(OW)(GSI),:F=94.972SL2-4832.2SL+833435(R2=0.4197);F=83.823SW+14084(R2=0.3511);2603:F=507.54OW+13190(R2=0.7262);F=11.623GSI2+65.472GSI+15799(R2=0.5741)2.3.3生殖力与生物学指标的多元线性回归分析(F)(SL)(SW)(OW)(GSI),:F=2375.54+525.67SL-31.44SW+199.43OW+261.74GSI,0.6478,F,44P0.20300.36880.20070.2188,0.05,,44Fig.4QuantitydistributionoftheeggdiameterinA.ommaturus2.41960egg,40.50~1.86mm,1.220.24mm1.0~1.6mm,69.68%1.2~1.4mm,38.45%2.5IV,,6.7~14.0cm,0.8~4.5cm,!!,#∃,,PlateMicrostructureofovaryofA.ommaturus1.∃;2.!;3.!;4.!;5.;6.!1.Theoocyteinphase∃;2.Theoocyteintheearlyphase!;3.Theoocyteinphase!;4.Theoocyteinthelatephase!;5.Theoocyteinphase;6.Theovaryatstage!,3,,,(1,2);,,(3);,,,(4),,,2,(5)3[10]3052~67896egg,18065egg;[5]8289~59078egg,22160egg;6328~59878egg,24607egg,,,2612010[14-15],33,(Ctenogobiusgiurinus)[16](Tridentigertrigonocephalus)[17](Bostrichthyssinensis)[18](Boleophthalmuspectinirostris)[19];3Tab.3Theindividualfecundityofdifferen