生态与农村环境学报

©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(3):246-250JournalofEcologyandRuralEnvironmentPenicilliumoxalicumIM-31,1,1,1,2,1(11,210046;21,210042):1(AAP)IM-3,18SrDNA,IM-3(Penicilliumoxalicum)14d,P.oxalicumIM-34116%1411%(IMI),(THI)(NIT)HPLCLC2MS/MS,N-IM2-1IM1-3:(Penicilliumoxalicum);;;:X131;O626:A:1673-4831(2010)03-0246-05MetabolismofChloronicotinylNeonicotinoidInsecticidesbyFungiPenicilliumoxalicumIM-3.JIWei2wei1,CHENTing1,SANGQi1,DAIYi2jun1,GEFeng2,YUANSheng1(1.CollegeofLifeScience,NanjingNormalUniversity,Nanjing210046,China;2.NanjingInstituteofEnvironmentalSciences,MinistryofEnvironmentalProtection,Nanjing210042,Chi2na)Abstract:Astrainoffilamentousfunguscapableofdegradingacetamiprid,aninsecticideofchloronicotinylneonicotinoidseries,wasisolatedfromsoilandnamedasStrainIM-3.IM-3wasidentifiedasPenicilliumoxalicumbymorphologyand18SrDNAsequenceanalysis.Inthemediumofmineralsalts,incubatedfor14days,P.oxalicumdegraded4116%ofAAPand1411%ofimidacloprid;butnilofthiaclopridandnitenpyram.HPLCandLC2MS/MSanalysisrevealedthatthemetabolicpathwayofAAPbyP.oxalicumIM-3wasN2demethylationofAAPtoformIM2-1andoxidativecleavageofcyanoiminemoietytoformIM1-3.Keywords:Penicilliumoxalicum;chloronicotinylneonicotinoidinsecticides;biodegradation;metabolicpathway:(200809092):2009-07-09E2mail:[e-mail],,8,(imidacloprid,IMI)(acetamiprid,AAP)(thiacloprid,THI)(nitenpyram,NIT)6-[1-2],,,,,,[2]ANHALT[3]Leifsoniasp.PC-21IMI;PANDEY[4]Pseudomonassp.1GIMI;YAO[5]Pseudomonassp.FH2AAPStenotroph2omonasmaltophiliaCGMCC111788AAPN-IM2-1[6-7],,,,AAP[8],AAP,AAP,3IMITHINIT©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:,200g,1L30min,,10g;:Na2HPO412H2O5134gL-1KH2PO41136gL-1NH4Cl10100gL-120100gL-1AAP0150gL-1,10mLL-1pH712;:CaSO412H2O0140gL-1H3BO30130gL-1CuSO45H2O0104gL-1KI0110gL-1FeSO47H2O0110gL-1MnSO47H2O0120gL-1NaMoO42H2O0120gL-1,10mLL-120gL-111520min112:1g,10mL,1mL50mL250mL,30220rmin-114d,100,PDA,,,30,PDA,AAP,:18SrDNADNAFREDERICK[9]18SrDNAHUREX[10],ITS4:5-tcctccgcttattgatatgc-3,ITS5:5-ggaagtaaaagtcgtaacaagg-3PCR,18SrDNAGenbankBlastnMega410,neighbor2joining113PDA,3010mL100mL4,500mgL-1IMI500mgL-1AAP500mgL-1NIT200mgL-1THI30220rmin-113000rmin-110min,,0122m,5,HPLC114HPLCLC/MSHPLC:Agilent1200(USA);AgilentHC2C18(250mm,416mm);20L;30;AgilentG1314A;IMIAAPTHINIT269235242270nm;0122m011mLL-1,IMIAAPTHINIT[V()V()]7525703065358515,1mLmin-1LC/MS2MS:Agilent1100-(LC2MSDTrapSL),;,HPLC,1mLmin-1;(ESI),2211AAP-,PDA©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(3),IM-3P.oxa2licum99%IM-3P.oxalicum,P.oxalicumIM-318SrDNAGenbankEF4906912IM-3Fig.2SporemorphologyofStrainIM-31000,18SrDNAGenBank3IM-3Fig.3PhylogenetictreeofStrainIM-3212AAPHPLC,,P.oxalicumIM-3AAP2,5163561313min(4),54P.oxalicumIM-3AAPHPLCFig.4HPLCspectrumofmetabolismofAAPbyP.oxalicumIM-31(m/Z)19810(2M),IM1-3[11],(m/Z)15812220911(M+H),S.maltophiliaN-IM2-1[7],194101671012611,P.oxalicumIM-3AAPN-IM2-1IM1-3213P.oxalicumIM-3IMITHINITAAPP.oxalicumIM-34IMITHINITAAP6AAP,:y=448121e-010395x(r2=019639),010395,1715d14d,AAP01856mmolL-1,©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(NyCCN)IM1-3,THI,P.oxalicumIM-3THI,AAPTHI,THI,AAP,AAPP.oxalicumIM-3THI(amideTHI)[12],P.oxalicumIM-3THIP.oxa2licumIM-3AAPN-,NIT,P.oxalicumIM-3NITN-,S.maltophiliaIMI,AAPN-,P450[7],P.oxalicumIM-3AAPN-,IMI,IMIIMI,HPLCIMI(ureaIMI),P.oxalicumIM-3IMI,NITP.oxalicumIM-3AAPIM1-3IM2-1IM2-1AAP1[7],IM1-3,[10],P.oxa2licumIM-3AAPIMIIMI1olefinIMI[6]AAPIMIAAP110812d,IM1-4(IM1-3N-)[7]P.oxalicumIM-3AAP,,AAPP.oxalicumIM-3IM1-3,IM1-4AAP,YAO[5]Pseudomonassp.FH2,P.oxalicumIM-3AAP:[1]KROHNJ,HELLPOINTNERE.EnvironmentalFateofImidaclo2prid[J].Pflanzenschutz2NachrBayer,2002,55(suppl.):3-26.[2]ELBERTA,HAASM,SPRINGERB,etal.AppliedAspectsof©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[J].PestManagementSci2ence,2008,64(11):1099-1105.[3]ANHALTJ,MOORMANTB,KOSKINENWC.BiodegradationofImidaclopridbyanIsolatedSoilMicroorganism[J].JournalofEnvironmentalScienceHealth:PartB,Pesticides,FoodContami2nants,andAgriculturalWastes,2007,42(5):509-514.[4]PANDEYG,DORRIANSJ,RUSSELLRJ,etal.Biotransfor2mationoftheNeonicotinoidInsecticidesImidaclopridandThiame2thoxambyPseudomonassp.1G[J].BiochemicalandBiophysicalResearchCommunications,2009,380(3):710-714.[5]YAOXH,MINH.Isolation,Char