进水浓度或HRT变化时微好氧处理微生态机制研究

HRT1,2,1,2,13,2(11,,100875;21,100037):COD(110001500030000mgPL)(HRT42h25h),2(FISH2FCM)2(PCR2DGGE)BiologFF,CODHRT.,4,9919%.COD,210gPL713gPL,COD213kgP(kgd)117kgP(kgd),21052119,41424145;HRT,713gPL610gPL,COD117kgP(kgd)218kgP(kgd),2119414501794136.,CODHRT.:;FISH2FCM;PCR2DGGE;Biolog;;HRT;:X703.1:A:025023301(2008)1123087206:2007211219;:2008201221:(973)(2006CB403306):(1984),,,,E2mail:[e-mail]3,E2mail:[e-mail]2ecologyMechanismonMicroaerobicTreatmentPerformanceatVariousInfluentCODConcentrationsorHRTsHANHui1,WANGXiao2dan2,ZHANGYan2yan1,ZHAIZhen2hua2,ZHENGShao2kui1,LIYan2hong2(11StateKeyLaboratoryofWaterEnvironmentSimulation,SchoolofEnvironment,BeijingNormalUniversity,Beijing100875,China;21CollegeofLifeScience,CapitalNormalUniversity,Beijing100037,China)Abstract:Alab2scalecontinuousexperimentwasdesignedtoinvestigatetheeffectofinfluentCODconcentrations(11000,15000,30000mgPL)orhydraulicretentiontime(HRT42,25h)onthemicroareobictreatmentperformanceofsimulatedhigh2strengthorganicwastewater.Themolecularmicrobiologicaltechnologies,includingFluorescentin2situhybridization2FlowCytometry(FISH2FCM),PolymeraseChainReaction2DenaturingGradientGelElectrophoresis(PCR2DGGE),andBiolog2FFassaymethod,wereusedtodetectthevariationofmicrobialcommunityintheaeratedcolumnduringthefourpseudo2steady2stateperiods.Theyeastcontentsremained9919%throughouttheoverallexperimentalperiodsaccordingtoFISH2FCM.IncreasinginfluentCODconcentrationbroughtonarisingMLSS(2102713gPL)andareducedspecificCODremovalrate[2132117kgP(kgd)],structural(PCR2DGGE)Pmetabolic(BiologFF)diversityindexvaluesoffungalcommunityintheaeratedcolumnhadanincreaseof(210522119)P(414224145).ShorteningHRTbroughtonareducedMLSS(7132610gPL)andarisingspecificCODremovalrate[1172218kgP(kgd)],structural(PCR2DGGE)Pmetabolic(BiologFF)diversityindexvaluesoffungalcommunityhadadecreaseof(211920179)P(414524136).IncreasinginfluentCODconcentrationorshorteningHRThasanabsolutelyadverseeffectonthemicroaerobictreatmentperformanceandmicro2ecologyintheaerationcolumnalthougheitherofthemcanincreaseinfluentCODloadinguptoahigherlevel.Keywords:micro2aerobic;FISH2FCM;PCR2DGGE;Biolog;high2strengthorganicwastewater;HRT;micro2ecology[(DO)015mgPL]SO2-4,SO2-4,[1,2].,,(hydraulicretentiontime,HRT)CODCOD.HRT,[35].,,,HRT,HRT.2911200811ENVIRONMENTALSCIENCEVol.29,No.11Nov.,2008,2(polymerasechainreaction2denaturinggradientgelelectr2ophoresis,PCR2DGGE)PCR2(singlestrandconformationpolymorphism,SSCP)[610],Biolog[1114],HRT.,,HRT,.11.1(111%310%)(NH4)2SO4(015%)KH2PO4(011%)(pH515).1(012m2m,1517L),,.,Candidatropicalis,,MLSSDOCODpHMLSS,DO015mgPL,CODHRT4,3HRT42h,COD110001500030000mgPL,HRT25h,COD30000mgPL.COD5%,12,,,.DODO(55P12FT,YSI),pH(pHB24,),4000rPmin10min[15]COD,105MLSS.1.21.2.1FISH2FCM2(fluorescentin2situhybridization2flowcytometry,FISH2FCM)[16,17]4,EUB338[18],52GCTGCCTCCCGTAGGAGT23,5Cy5,PF2[19],52CTCTGGCTTCACCCTATTC23,5Cy3,2.FACSCalibur(BectonDickinson,USA),FL2(564606nm)FL4(653667nm)Cy3Cy5,(FSC2H)(SSC2H).CellQuest(BectonDickinson,USA).1.2.2PCR2DGGEMatrixETube(Q2Biogene),Bio101FastDNASpinKitforSoil(Q2Biogene)DNA,018%.PCR18SrDNAFF390(52CGATAACGAACGAGACCT23)PFR1+GC(52CCCCCGCCGCGCGCGGCGGGCGGGGCACGGGCCGAICCATTCAATCGGTAIT23)[20].25LPCR:215L10PCRbuffer,5mmolPLdNTP,5molPLFR1+GC,5molPLFF390,50ngDNA,215UTaq,ddH2O25L.PCR:952min;9530s,5530s,7230s,30;7230min.PCR115%,DcodeTMd(Bio2Rad)DGGE.DGGE()40%55%,:1TAE(pH815),130V60615h.(Sigma)[21]100,QuantityOne4131(Bio2Rad)DGGE,,(S)DGGE,(Shannon2Wiener):880329H=-(PilnPi),Pii.1.2.3BiologFF,BiologFF(150L),25,12h490nm.(AWCD)AWCD=(Ci-R)P95,,Ci,R,240hBiolog,(Ci-R0125[22]),(Shannon2Wiener)H=-(PilnPi),Pii(Ci-R),Pi=(Ci-R)P(Ci-R).22.1CODHRT1.,COD(123)HRT(34)COD,2MLSSCOD:HRT(42h),COD11000mgPL30000mgPL[COD615kgP(m3d)17kgP(m3d)],MLSS(210gPL713gPL),COD213kgP(kgd)117kgP(kgd);COD(30000mgPL),HRT42h25h[COD17kgP(m3d)29kgP(m3d)],MLSS(713gPL610gPL),COD117kgP(kgd)218kgP(kgd).2.2142FL22FL4.EUB338Cy5FL4,PF2Cy3FL2,1,,.,CellQuest2.,4011%,,.1Table1Treatmentperformanceoflab2scalecontinuousexperimentatfourphasesCODPmgL-1HRTPhCODP%Pkg(m3d)-1MLSSPgL-1CODPkg(kgd)-1111000427126.50112.00.22.30.3215000428758.90113.60.32.10.133000042712170117.31.01.70.243000025594290136.00.62.80.22P%Table2ContentsofyeastandbacteriumcellsinaeratedcolumnatfourexperimentalphasesP%19919401062991990101399199010149919801022.3FISH2FCM4,,PCR2DGGEBiologFF4,DGGE2,Biolog3,3.4PCRPCR(390bp),DGGE2(W0C1tropicalis980311:HRT1234:1234,12FL42FL2Fig.1FCMdotplot(FL2vs.FL4channel)forfourbacterium2yeastbinarysamplesW0:C.tropicalis2PCR2DGGEFig.2PCR2DGGEresultforfungalcommunityinaeratedcolumn3AWCDFig.3ChangeinAWCDwithdurationofincubationforfourphasesDGGE).,,DGGE,3,3,09032924,,,C.tropicalis,3PCR2DGGE.DGGE,C.tropicalis3,C.tropicalis18SrDNA,DGGE11;,C.tropicalis,,(),.3,4Shannon2Wiener3124,COD,,HRT,.BiologFF[23].34BiologFFS,(24ht72h)AWCD3142,3Biolog.AWCD,Biolog,AWCDBiolog[24,25],,CODHRTBiolog.HRTCOD.CODHRT,,,Biolog,BiologFF,AWCD,3Biolog.,Biolog,.3,4Biolog3142,,CODHRT,,3COD(HRT),.3Table3Microbialcommunitydiversityinaeratedcolumnatfourphases(S