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2006,Vol.27,No.123261Fig.1StructureofglycyrrhizicacidCOOHCOOHHOOHOHOHOHOOOOOHOOC2006-08-16(xjj200411)(1973-)121(1.7100492.710032)40%42020min13kHz1:10StudyonOptimizationofTechnologyforExtractingGlycyrrhizicAcidfromLiquoriceLIJian-jun1GUORong2MOXiao-yan1(1.KeyLaboratoryofBiomedicalInformationEngineeringofMinistryofEducation;SchoolofLifeScienceandTechnology,Xi'anJiaotongUniversity,Xi'an710049,China2.SchoolofOptoelectronicalEngineeringXi'anInstituteofTechnology,Xi'an710032,China)AbsractThesupersonic-assistedextractingtechnologyofglycyrrhizicacidfromliquoricewasresearched.Combinedartificialneuralnetwork(ANN)withtraditionalorthogonaldesign,anewmethodoftestdataanalyzing,processingandoptimizingwasputforward.Withthismethodthetestinformationcanbefullyused,thechangeregularityofeachfactorcanbeanalyzedandtheoptimumcombinationcanbefound.Byusingthisnewmethod,theoptimumextractionconditionwasobtainedasfollows:ethanolconcentration40%,supersonictime50min,extractingtemperature20,extractingtime20min,supersonicfrequency13kHzandratioofsolid-liquid1:10.OptimizingextractiontechnologybyANN,theexperimentalinformationcouldbedigoutcompletely.Applyingthispreferableextractingtechnology,thecostsofproductionwasreduced,theenergyconsumingwasdecreased,andtheglycyrrhizicacidcontentwasincreased.Theresultsshowedthatthisnewmethodwasreasonableandpracticable.Keywordsglycyrrhizicacidsupersonic-assistedextractionextractingtechnologyartificialneuralnetworkR932.12A1002-6630(2006)12-0326-05,[1],,1(HBV)(HbsAG)()HIV,[2,3]3272006,Vol.27,No.1265[4]11.198%(J2-MC)(DU640)BechkmanMitletter(RE-52A)(DZKW-4)(JZ7114)1.21.2.10.25g50ml50%12345ml50%250ml254nm50%C=0.0106A0.0041R=0.99971.2.250%254nm1.2.3CVR=100%(1)1000WR(%)C(mg/ml)V(ml)W(g)22.12.1.1L25(65)1(W/V)(min)()(%)(kHz)()11:820202013121:1040254026231:1560305030341:2080406040451:3010050705051Table1Designoftheexperimentsforinfluenceofdifferentultrasonicextractionconditionsontheextractyield2.1.22101A1B2C3D4E5F6A2B5C1D2E3F4DFCBEAA2B1C1D2E1F440%42020min13kHz1:1022.84%2.2BP22.2.1BP(BackPropagationNetwork)2Sxe11)x(f−+=2006,Vol.27,No.12328(W/V)(min)()(%)(kHz)()(%)ABCDEF11:820202013122.0022.00021:840254026220.9020.89931:860305030317.6019.59941:880406040419.7519.74951:8100507050517.0016.99961:1020255040518.1018.09971:1040306050120.6020.59981:1060407013218.9518.94991:1080502026319.4019.399101:10100204030422.3222.318111:1520307026420.8720.869121:1540402030517.5217.519131:1560504040119.9519.949141:1580205050217.6017.600151:15100256013318.5918.587161:2020404050320.6020.600171:2040505013419.5519.550181:2060206026519.8019.798191:2080257030118.1418.140201:20100302040220.4920.489211:3020506030218.8818.881221:3040207040319.1619.161231:3060252050418.5418.539241:3080303513520.6620.658251:30100405026118.6018.600I/5*19.4520.0920.1819.5919.9519.86II/519.8719.5518.8520.8919.9119.36III/518.9118.9720.0418.2918.8919.07IV/519.7219.1119.0819.5219.4920.21V/519.1719.4018.9618.8218.8718.62R0.961.121.332.61.081.59*IIIIIIIVVR2Table2OrthogonaltestandsimulationresultsofglycyrrheticacidextractioninliquoriceBPBPLM(Levenberg-Marquardt)[5]Funahashi3BP3BP662625(X1kX2kX3kX4kX5kX6k)k(X11X21X31X41X51X61)1(X12X22X32X42X52X62)2(X125X225X325X425X525X625)251142.2.2BP3292006,Vol.27,No.12[6]Hes-sianLMLMLMw=(JTJ+I)1JTet(2)E=et(3)JJacobianetGauss-NewtonELMeiJacobianJ(1)(2)W(k+1)EEEW(k+1)EW(k)2.2.3BPMATLAB(premnmx)[-1,1]MATLAB(sumsquareerror,SSE)0.000150000.01L-M126SSE2.26106122BPFig.2ThetopologicalstructureofBPneuralnetwork522.522.021.521.020.520.019.519.018.50.020.040.060.080.100.120.14(%)(W/V)3Fig.3Influenceofsolid-liquidratiofluctuationontheextractyield232221201918171620406080100(%)(min)4Fig.4Influenceofabstractiontimefluctuationontheextractyield2.2.4131:101:10480min20min20min522.021.521.020.520.019.520253035404550(%)()5Fig.5Influenceofabstractiontemperaturefluctuationontheextractyield2006,Vol.27,No.123302525660%40%40%21.521.020.520.019.5203040506070(%)(%)6Fig.6Influenceofethanolconcentrationfluctuationontheextractyield20.019.919.819.719.619.519.410152025303540455055(%)(kHz)7Fig.7Influenceofsupersonicfrequencyfluctuationontheextractyield22.522.021.521.020.520.019.519.018.5123456(%)()8Fig.8Influenceofsupersonictimefluctuationontheextractyield21:1020min2540%13kHz423.56%33.13.240%42520min13kHz1:1023.56%3.3[1].[D].,2003.[2],.[J].,2001,13(1):10-12.[3],.[J].,1995,26(1):39-44[4].[J].(),2004,30(5):519-523.[5],,.[J].,2001,22(1):77-83.[6],,.[J].,2003,54(6):783-789.713kHz82