高温过氧乙酸法提取羽毛角蛋白工艺的研究

20073222JournaloftheChineseCerealsandOilsAssociationVol.22,No.2Mar.2007112(1,200093)(2,450002),,:25%,128,20min,15,11%,90%,75.67%,82.87%,,,,2010,6000,2200[1],85%89%,,32[2],20,10[3],,,,;,,,,,,[4-5],,,,[6-9];,,:(T0503):2006-02-14:,,1968,,,,,,,,,,11.1:;:;:1.2SS-325:TOMYSEIKOCO.LTD;TDL-60B:;DZF-OB:1.31.3.1vvyy=W-W1W100%u(%),W20072(g),W1()(g)5min,,,5min(3000r/min),23,45,1.3.21.3.2.110g,70mL20%,,,,132,2030406090min;,1.3.2.210g,70mL10%15%20%25%,132,30min;,1.3.2.310g,70mL20%,117122127132,30min;,1.3.2.410g,305070100150mL20%,128,30min;,1.3.3,,1,L9(34),1/%/min/1254013219220301301731520128151.3.41.3.4.1Parsons[10],1g(60)250mL,75mL0.2%KOH,20min,50mL,10min(2700r/min);1/5(%)=15mL51001.3.4.2(PDP):1g,200mL,450.2%(0.036mol/L)150mL,,45,16h;,;,(CP):PDP(%)=-10022.12.1.12.1.1.1,,,[11],,,12,20min40min,,;30min,89.8%,;,,,,,,,[12],,,,,30min,;3060min89222,,,,,,30min122.1.1.234,10%20%,;20%,,(1)pH,,;,,,,[12](2),,,,,,,20%,98%,10%(1.0g);,,,34,,,,20%2.1.1.35656,,,132(200kPa),;1151279920072,127,,:(1),(2)(3),,(4),,,,1272.1.1.478,07,;17,95.6%,9.4%;17,,:(1),,,,,,,,(2),[12],(3)17,,,pH3,,78,,,172.1.22,3,92/%//min/%/%/mL125132409198.61092251303074.295.77032512820511.389.8404201323056.882.4555201302096.591.1946201284075.992.2807151322077.172.3718151304056.162.9539151283096.787.910099:(1),,,,,;,,,,(2),,,,10:001222,1011:(1),,,,,,(2),,,130,,,,;,,,11:(1);(2);(3):25%,128,20min,15337.91%0.61%3.97%83.59%75.67%82.87%2.2,44/%/%/%84.7912.1429.1283.5975.6782.87:83.59%,84.79%,12.14%75.67%,63.53%;29.12%82.87%,53.75%,,,2.3(140),,55/%/%/%65.8821.8652.1853.1459.9373.4583.5975.6782.87,83.59%,;75.67%,15.74%,53.81%;82.87%,10%,,30%,101200723,,,,,(),,,:25%12820min15;11.3%,90%,,:75.67%,82.87%,,:,,,,,56h,8h,30min,,,,5%,11.3%,,,,,,(140),:84.79%,12.14%,29.12%;65.88%,21.86%,52.18%;53.14%,59.93%,73.45%;83.59%,75.67%,82.87%,,30%,15%,10%;,17%,50%,30%;,,,,,,::,:,,,44.1:25%,128,20min,154.2,89.8%,11.3%,:83.59%,75.67%,82.87%4.3,,30%,15%,10%;,17%,50%,30%;,4.4:,,,,,201222[1].:[].:,2001[2].:.,2003(2):103-104[3]..,2002,11(4):13-15[4],,..,1998,13(4):16-19[5]..,2000,21(6):1-5[6]SFTimmons,CRBlanchard,RASmith.MethodofMakingandCross-linkingKeratin-basedFilmsandSheets,U.S.Patent,6124265,2000-09-26[7]SFTimmons,CRBlanchard,RASmith.MethodofMakingandCross-linkingKeratin-basedFilms,SheetsandBulkMaterials,WOPatent,9926570,1999-06-03[8]SFTimmons,RASmith.PorousandBulkKeratinBio-polymers,U.S.Patent,6159495,2000[9]..,1998(6):27-28[10]ParsonsCM.Soybeanproteinsolubilityinpotassiumhy2droxide:Aninvitrotestofinvivoproteinquality[J].JAnimalSic.1991(69):2918-2924[11],..:,1989[12],,..:,1999ExtractingFeatherKeratinwithPeroxyAceticAcidYangBo1YangGuang1WangYanling2(UniversityofShanghaiforScienceandTechnology1,Shanghai200093)(HenauAgriculturalUniversity2,Zhengzhou450002)AbstractTheperoxyaceticacidwasusedforfollowingextractionoffeatherkeratin:treatingthefeatherinper2oxyaceticacidsolutionandthenconductingthereactioninanautoclaveundercertaintemperatureandpressure.Theresultsshowthatwithconditionsofperoxyaceticacid25%,temperature128,time20min,andfeed/solutionratio15,thefeatherkeratinyieldis11%,thesolubilityofthefeatheris90%,thecontentofthesolubleproteinis75.67%,andthedigestibilitybypepsininvitroisupto82.87%.Keywordsfeatherkeratin,peroxyaceticacid,extractionmethod(96)sulfategroupssignificantly.TheresultsindicatethatyeastzinccanimprovetibiaZnabsorption,andtheinhibitionofyeastzinctoabsorptionofCuandFeislowerthanthatofzincsulfate.Keywordsyeastzinc,zincsulfate,broiler,traceelement,absorption301