工业废水生物毒性监测与综合评价

26220074Vol.26No.2JournalofLiaoningTechnicalUniversityApr.20072006-03-28(Z2006407)[e-mail]1008-0562(2007)02-0293-03112(1.0660042.110031)X832AMonitoringandintegratedassessmentofbiologicaltoxicityofindustrialwastewaterZHOUXiu-yan1WANGYan1HANGui-chun2(1.NortheasternUniversityatQinhuangdao,Qinhuangdao066004,China;2.LiaoningEnvironmentalMonitoringCentralStation,Shenyang110031,China)AbstractInordertostudytheapplicationoftoxicitytestswithaquaticorganismsinmonitoringandmanagementofindustrialwastewater,biologicaltoxicitytestswereconductedonBrachydaniorerio,luminescentbacteriaandViciafabaofthesourcesofindustrialwastewaterinLiaoning.Theresultsindicatethatbiotoxicityindices,effluentvolumeofindustrialwastewaterandpollutantshavecloserelations.Thebiologicaltoxicityofthetypicalindustrialwastewatershouldparticularlybepaidmoreattentiontoandpollution-controlmeasuresshouldbetaken,otherwise,itwillaffectdegradationofthewaterqualityandaquaticbiosafety.KeywordsindustrialwastewaterbiologicaltoxicityluminousbacteriaassessmentBulichA.[1]PhotobacteriumPhosphoreumMicrotoxBiesingerK.[2]DaphniamagnaWalshG.[3]algae[4]T3T9171[5]Vibrioqinghaiensissp.-Q67T3200221()1.2.1GB/T15441-19958001000mv3%26294NaClpH20±5HgCl215minEC500.080.12mg/LHgCl2-21.2.2[6]GB/T13267-9196h57101000mL481224487296h96hLC501.2.3[7]24h241.5cm564h22h1cm316~12h1NHCl60810min1h1mm45%PEEP(PotentialEcotoxicEffectsProbe)[8]PEEP⎥⎦⎤⎢⎣⎡⎟⎠⎞⎜⎝⎛+=∑QNTUnPEEPNi11log()5050100ECLCTUi=TUiNnQ(m3/a)21LC5011.4090.033.0010010.0043.67(1)Tab.1biologicaltoxicityindexofindustrialwastewaterLC50/%/%/0.1%114.6496.7043.67219.9834.5013.00372.0030.2517.50490.0316.8419.00520.0095.5023.08674.1310022.00741.6099.8515.03835.523.0012.00943.2065.0014.471051.7430.9713.0011-10010.001272.0832.9623.801346.5054.5022.431431.6257.5016.001518.6050.0020.001658.684.8116.001749.1098.4212.821837.4082.5513.921930.4083.7032.5420-88.6531.602111.4025.4112.00[9-11]21123456123456789101112131415161718192021123456Lc501Fig.1classificationofbiologicaltoxicityanddegreeofpollutioninthetypicalindustrialwastewater229521LC5068.4%81.0%61.9%42.9%Tab.2classificationstandardofbiologicaltoxicityanddegreeofpollution0.1%10NAT1020—3050—10010-18330—5010—5018-30450—801—1030-40580140PEEP21PEEP6.04102EPAPEEP[12]PEEP621PEEPPEEP(Q)COD(COD)0.580.57(3)PnCODPEEP0.580.52LC50PnTab.3correlationcoefficientofbiologicaltoxicityindexofthetypicalindustrywastewaterinLiaoningQCODPnPEEPLC50COD0.2Pn0.030.51PEEP0.580.570.2LC500.360.210.110.21-0.080.580.250.520.0010.310.460.030.120.430.14121LC5068.4%81.0%42.9%2LC503PEEP6.04104COD[1]BulichAA,IsenbergDL.Useoftheluminescentbacterialsystemfortherapidassessmentofaquatictoxicity[J].ISATransactions,1981,20(1):29-32.[2]BiesingerKE,ChristensenGM.Effectsofvariousmetalsonsurvival,growth,reproduction,andmetabolismofDaphniamagna[J].JFishResBoardCan.1972,29(12):1691-1700.[3]WalshGE,LowellHB.Toxicityoftextilemilleffluentstofreshwaterandestuarinealgae,Crustaceansandfishes[J].Enviromentalpollution(Ser.AEcol.Biol.)1980,21(3):169-179.[4],,.[J].,1993,13(4):435-441.[5],,,.(Vibrioqinghaiensissp.-Q67)[J].,1998,18(1):87-91.[6].[M].:,1986.95.[7].[M].:,1993.[8]TakashiKusui.StrategiesformanagementofwaterenvironmentsinNorthAmerica[J].AquaticEnvionmentAcad,2000,23(7):20-24.[9].[M].,1992.[10],,.[J].,1992,2:23-30.[11]RoncoAE.Developmentofabioassayreagentusingphotobacteriumphosphoreumasatestforthedetectionofaquatictoxicants[J].WorldJournalofMicrobiologyandBiotechnology,1992,8:316-318.[12]USEPA.Technicalsupportdocumentforwaterqualitybasedtoxicscontrol,EPA,officeofwater[M].Washington,U.S.A.1985.024681012123456789101112131415161718192021PEEPPEEP2Fig.2distributionofPEEPintheindustrialwastewateroftypicalpollutessources