实验六动物染色体标本的制备与观察

1,31.2.(10m1)(1m1)(400m1100m1)1.228g(TrisodiumcitrateAR)2.123.200gml(cochicine)4.(Giemsa)(PH6.8)Giemsa(Giemsastain)1g(glycerineAR)33ml(methylalcoholAR)45ml60652h5.0.067mol/L(pH6.8)Na2HPO4.12H2O11.81g(Na2HPO4.2H2O5.92g)KH2P044.5g1000m16.(Giemsa)(pH6.8)1ml9ml0.067mol/L(pH6.8)7.(AR)8.(Aceticacidglacial,AR)90.4KCl(potassiumchlorideAR)1.4gg34h2.0.61ml21ml5#10ml3.0.4%KCl810ml3710min4.1000rmin8min3(31)5ml20min2320min5.2330min6.110Giemsa0.21m110min7.15sGiemsa1.Giemsa2n=402.401.4gg34h50%80%2.23.44.20min5.2448h46.1.2.