水稻转基因技术与转基因水稻

©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:2005-08-0920055::;;,1983,2004,()11250hm2,1000,1988,,(),1985;1988;1990ChinsurahBoro;1990;1991,,,,1993,,1.2080,DNA,DNA,,1989,,,,,2.,:,10%30%,,,,,DNA,3.PEG(PEG)pHDNA,,DNA,,,,DNAPEG,,,,,,,,,?:1).,SouthernPCR2).,NorthernRT-PCR(reversetranscribedPCR)3).,::;:(),Western;4).,:gusnosocs,,,1.,,,5©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(phosphinothricin)(glyphosate)()981046,2.,,,(Bt)(PI)3,BtcryIA(b)cryIA(c)2000B12000B6,1995,Bt,199812,2000B12000B663Bt63,3.,,,Xa21PibCecropinBCP,,TuJMXa21IR7210676311,WangPib,BCecropinB1111,4.,,,,,,,():1-(mtlD)6-(gutD)(CMO)(BADH)CMOBADHmtlDgutD11,(T1111016),0.5%CMOBADH277,T277()5.,,,,(AGP),(309),5%AGP10,C4PEPCPPDKNADP-ME,,35%6.,MOC111,,,,AC/DSDNAPICre-loxI-ScelDNA,,,,,,,,,1/2,,,,:6©1994-2010ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:,,,,,4:1.,2,24,6,(Pibgm)22.92-183ZC15,,6Pi25(t),12Pi24(t),Pi24(t)Pi25(t),Pi25(t)23.Pi25(t)Pi26(t),QTL,4.,Pi25(t),Pi25(t),,1..,2000,(5):39422,..,2001,18(3):2832873ChristouP,etal.ricegeneticengineering:areview.TrendsBiolTechnol,1992,10:2392464,.CecropinB.,1999,13:1141165,..,2002,38(2):1051106,.C418-Xa21.,2001,17(8):3803847,..,2002,38(2):1051102005826,1401,,120,1,,,,,,,,27.8g,90.91%,685kg,,19AR8006,,112,1022,,,2004,2005,,12(),28,1,1,,,1,,,,,17