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,D3,D3BP897Ò,,D3[][1],,1[J].中国神经科学杂志,2003,19(6):405-4101[2],1[J].首都医药,2003,10(18):12-151[3],,1D3[J].中国药物依赖性杂志,2001,10(2):86-881[4],1[J].中国行为医学科学,1998,7(4):315-3181[5]ShaferRA,LevantB1TheD3dopaminereceptorince-llularandorganismalfunction[J].Psychopharmacology,1998,135:1-161[6]ChildressAR,O.BrienCP1Dopaminereceptorpartialagonistscouldaddressthedualityofcocainecraving[J].TrendsPharmacolSci,2000,21:6-91[7]1D3[J].,2002,26(2):53-561[8]PillaM,PerachonS,SautelF,etal1Selectiveinhibitionofcocaine-seekingbehaviorbyapartialdopamineD3re-ceptoragonist[J].Nature,1999,400:371-3751[9]FollBL,SchwartzJC,SokoloffP1DopamineD3recep-toragentaspotentialnewmedicationfordrugaddiction[J].EurPsychiatry,2000,15:140-1461[10]SchwartzJC,DiazJ,PilonC,etal1Possibleimplica-tionsofthedopamineD3receptorinschizophreniaandinantipsychoticdrugactions[J].BrainResBrainResRev,2000,31:277-2871[11]Aston-JonesG,DruhanJ1Breakingthechainofaddic-tion[J].Nature,1999,400:317-3191[12]HacklingA,GhoshR,PerachonetS,etal1N-(X-(4-(2-Methoxyphenyl)piperazin--lyl)alkyl)carboxamidesasdopmineD2andD3receptorligands[J].JMedChem,2003,46:3883-38991[13]LeopoldoM,BerardiF,ColabufoAN,etal1Structure-affinityrelationshipstudyonN-[4-(4-arylpiperazin-1-yl)butyl]arylcarboxamidesaspotentandselectivedopamineD3receptorligands[J].JMedChem,2002,45:5727-57351[14]WoodMD,BoyfieldI,NashDJ,etal1EvidenceforantagonistactivityofthedopamineD3receptorpartialag-onist,BP897,athumandopamineD3receptor[J].EurJPharmacol,2000,407:47-511[15]WickeK,GarciaLadonaJ1ThedopamineD3receptorpartialagonist,BP897,isanantagonistathumandopamineD3receptorandatratsomatodendriticdopamineD3receptor[J].EurJPharmacol,2001,424:85-901[16]JoyceJN,YoshimotoK,UedaS1Thezittermutantratex-hibitslossofD3receptorswithdegenerationofthedopaminesystem[J].Neuroreport,2000,11:2173-21751[]2004-10-14[]国家自然科学基金项目(30271491);江苏省自然科学基金项目(BK2002119)*:张正行,教授;:药物的光谱和色谱分析;Tel:025-83271256;E-mail:zhangzx@cpu1edu1cn生物体内一氧化氮的检测方法及其应用定天明1,陈坚2,张正行1*,张奕华3(11中国药科大学药学院药物分析教研室,江苏南京210009;21新疆医科大学药学院,新疆乌鲁木齐830054;31中国药科大学新药研究中心,江苏南京210009)[],,2005年第29卷第5期第221页ProgressinPharmaceuticalSciences药学进展##2005,Vol.29,No.5221ESR(),,,[];;[]O655;O61316[]A[]1001-5094(2005)04-0221-06TheMethodsforDeterminationofNitricOxideinVivoandTheirApplicationsDINGTian-ming1,CHENJian2,ZHANGZheng-xing1*,ZHANGY-ihua3(11DepartmentofPharmaceuticalAnalysis,ChinaPharmaceuticalUniversity,Nanjing210009,China;21DepartmentofPharmacy,XinjiangMedicalUniversity,Urumchi830054,China;31CenterofDrugDiscovery,ChinaPharmaceuticalUniversity,Nanjing210009,China)[Abstract]Nitricoxide(NO)playsanimportantroleinphysiology,pathophysiologyandpharma-cologyoforganism1ThedeterminationofNOinvivoishelpfulforitsresearchinpharmaceuticalfield1ThebiosynthesisofNOwasbrieflyintroduced.ThemethodsofdeterminationofNO,suchaselectrochemistry,chemiluminescence,fluorescence,spectroscopic,electronspinresonance(ESR)spectroscopyandoptica-lfiberchemical(biological)sensorswereemphaticallyreviewed,andtheappl-icationsofthesemethodsinrecentyearswerealsodiscussed1[Keywords]Nitricoxide;Determination;invivo(NO)[1~3]NONONONONO,NO1NONO,NO,,,NONO(NOS)L-,L-,NOS,NOS(cNOS)NOS(iNOS)NO,10-12mol/L,;NO10-9mol/L,[4,5]L-NG--L-(L-NMMA)NG--L-(L-NNA)NOS,NO,L-[6]2NONO,NONO2~6s,,NONO211()NO()O2,NO,:NO+2H2OyNO3-+4H++3¶,,NONafionNO2-NO3-NO,NO2-NO3-##2222005,Vol.29,No.52005年第29卷第5期第222页ProgressinPharmaceuticalSciencesNOMalinski[7]Nafion015Lm,,NO10nmol/L,10nmol/L~300Lmol/LNO,NO[8]NO,,,6Lmol/L,10~800Lmol/L(),Nafion,,,,(10ms),NO,NO,,,N,N-[Co(salen)][9~11]N,N-[Fe(salen)][12,13]N,N-2,6-[14,15][Cu(Ò)L]Cl(L1,8--1,3,6,8,10,13--14--4)[16][Nc,Nd-(1,3-)(1,2-)-N,Nc,Nd,NÊ][17][18]Hoshino[19],Co(salen),COO2NO,[15],NOCo-DAPBA-NO(DAPBAN,N-2,6-),NO,Co-DAPBA,NONO,,,NO,,WorldPrecisionInstrument(USA)NOISO-NO,3nmol/L,100~1000nmol/L,PBS(011mol/LNa2HPO4-011mol/LKH2PO4)NO,,,,21221211,NONO2#,NO2#600~3000nm(3000nm),NO2,NO+O3#yNO2#+O2NO2#yNO2+hC,NO2#NO,,NO5pmol/L:¹NO,,,NO;º;»,NO2CO2CONH3;¼,21212(Luminol)NO,NO(H2O2)ONOO-,ONOO-Lu-minol(2-),Luminol,NO,NO2-NO3-,NO,,Kikuchi[20]NO(85?11)fmol/(g#min),10-13mol/L,Evmiridis[21],NOSNO,pH,21321311GriessNONO2-,NO2-Griess,520~560nm,NO2-,NO,,NO3-,,,[22],A-NO2-,NO[23],,2005年第29卷第5期第223页ProgressinPharmaceuticalSciences药学进展##2005,Vol.29,No.5223,NO3-NO2-,NO2-Griess,540nm,NO[24]NO,0~1000Lmol/LDacres[25][Cu(Ò)ECR]NO,(pH714),0~60Lmol/L,1124Lmol/L,NO[Cu(Ò)ECR],NO,01227Lmol/L21312Hb()NOHbO2CO,HbO2:Hb(Fe2+)+NOyHb(Fe2+)NOHb(Fe2+)O2+NOyHb(Fe3+)NO3-Hb(Fe2+)NO406nm,Hb27nmNOKelm[26]NO[Lmol/(L#min)][Lmol/(L#min)]1124Lmol/L1112Lmol/LNOONOO-NO2-NO3-,,NO214Misko[27],NO2-2,3-(2,3-diaminonaphthalene,DAN),1-(H)-[1-(H)naphtho-triazole],(pH10),365nm,450nm,NO2-,NO,[28]NO,4-NO2-,Na2S2O3,(pH13),3--4-,(Ex)340nm(Em)453nm,,4-Ohta[29]Kder[30],2@10-5~2@10-8mol/L,NO2-NO215ESRNOHbA-,ESR,NONO,ESRCar-ini[31]ESRNCX-4016(NO-ASA)HbNO,NOHb,,ESRNO,ESR,NO,NO216,,,,,,,,[32~34]NO:Luminol,Luminol,,,CCD,NOZhou[35]NONO,NOLuminol,NO,##2242005,Vol.29,No.52005年第29卷第5期第224页ProgressinPharmaceuticalSciences113Lmol/L,8~17s[36],,,815@10-12~117@10-10mol/L,117@10-12mol/L,,NO,,NO,Susan[37]NO,NOSusan[38]N
本文标题:生物体内一氧化氮的检测方法及其应用-定天明
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