植物谷胱甘肽研究进展

2010,22(4):118~121ActaAgriculturaeJiangxi李志刚1,许自成1*,苏永士2,陈彦春2,胡皓月1,张蕊1,杜娟1:2010-02-08:(HYKJ200718);(SYKJ200801):(1985-),,,,:*:(1.,450002;2.,472000):谷胱甘肽是植物中普遍存在的硫醇,在还原硫的贮存和运输蛋白质合成及植物抗逆性方面均有重要作用介绍了谷胱甘肽的测定方法和在植物中的生理作用,概述了谷胱甘肽在小麦水稻烟草果树及蔬菜上的研究进展并提出了对谷胱甘肽在植物体内合成降解运输的调控机制及对植物抗氧化作用机理研究的重要性:谷胱甘肽;植物;抗氧化作用:Q946:A:1001-8581(2010)04-0118-04ResearchProgressinPlantGlutathioneLIZhi-gang1,XUZi-cheng1*,SUYong-shi2,CHENYan-chun2,HUHao-yue1,ZHANGRui1,DUJuan1(1.CollegeofTobaccoScience,HenanAgriculturalUniversity,Zhengzhou450002,China;2.CompanyofSanmenxiaCity,HenanProvincialTobaccoCompany,Sanmenxia472000,China)Abstract:Glutathioneisanabundantandubiquitousthiolinplants,whichisveryimportantforthestorageandtransportofre-ducedsulphur,thesynthesisofproteinandthedefencereactionsagainstenvironmentalstressonplants.Themethodsfordeterminationofglutathioneinplantswereintroducedinthisessay,andtheresearchprogressesoftheglutathioneinwheat,rice,tobacco,fruitsandvegetableswerealsosummarized,theauthorconsideredthatthefurtherstudyofglutathionehadgreatsignificance.Keywords:Glutathione;Plant;Antioxidation(Glutathione,GSH)1929Hopkins[1,2],(L--L--)[3],C10H17O6SN3GSH,(),,,1(GSSG)(GSH)[2],,,,,,[4]2,HPLC,,2.1,,[5],0.1g/L,0.1~40.0g/L,99.87%,30min,,2.2305nm,,,305[6]Ellman[5,5c-(2-),DTNB][7],DTNBpH=8.0,412nm,GSH,,2.3,,,,,,,,[8]2.4HPLCHPLCGSHGSSG20,,,50Lmol/L[6]33.1(GSH)(GSSG),GSH/GSSGFahey[9],,35S-KGSSG,GSSG,,GSSGGSH,[10]3.2GSHRennenberg[11],,GSH,,GSH,GSHGSH,35S-,2,,,:67%,[12]3.3,GSH,GSH,GSH,,GSHGSH/GSSG,GSH/GSSG[13][14],GSH,,GSH,GSH[15],,GSH3d,GSH,,GSH,GSH3.4GSH,GSH(Phytochelatin,PC),Cd-S4-complex,,,,[16][17]Cd2+,Cd2+,GSH,,(GR)GSSGGSH,GSHAsA-GSH,H2O2,GSH3.5GSH#OHGSH,SHGSH,GSH,GSHGSHH2O2,,#OHGSH,[18][19]:H2O2,(GSH72%),,GSH/GSSG,,GSH44.1CO2[20]He-Ne[21]1194:,GSH,GSSG,GSH/GSSGGSHGSSG,GSH/GSSG,,,,[22],:,GSH,GSH,GSH,,GSHMDA,4.2,GSH,CaCl2GSH,CaCl2GSH,GSH,CaCl2,,GSH[23,24],[25],:,)GSH,,4.3,NC89,,:60d,GSH,,GSH[26][27],GSH:GSH,GSH,GSH25%60%,GSH,NC89JYH,GSH,:NC89GSH,30~70dGSH,70dJYHGSH40d,60d,NC89,NC89GSHJYH[28],,,,GR,[29,30]GSH,:GSH0~48h,48h,,GSH,,,,4.4,,(GSH+GSSG)GSHGSSG,GSH/GSSG,,GSSGGR,GRGSH,GSHGSH[31][32]:2d,,,,,GSH/GSSG,,,GSH,,GSH,GSH[33]5GSH,GSH,,:[1]ZetterstromR,EijkmanC,SirHopkinsG.TheDawnofVitaminsandOtherEssentialNutritionalGrowthFactors[J].ActaPaedia-trica,2006,95(11):1331~1333.[2],,.[J].,2004,29(5):42~45.[3]DixonDP,SkipseyM,GrundyNM.Stress-InducedProteinS-GlutathinonylationinArabidopsis[J].PlantPhysiology,2005,138(10):2233~2244.[4],,.[J].,2004,24(6):1119~1130.12022[5],.[J].,2001,(11):46~47.[6],.[J].,2007,(3):26~29.[7]EllmanGL.TissueSulfhydrylGroups[J].Arch.Biochem.Bio-phys.,1959,82(1):70~77.[8],,,.[J].,2004,14(1):68~70.[9]FaheyRC,DistefanoDL,KMcierGP,eta.lRoleofHydrationStateandThioldisulfideStatusintheControlofTheramlStabilityandProteinSynthesisinWheatEmbryo[J].PlantPlaysio.l,1980,65(5):1062~1066.[10]DavidsonBE,HirdFJR.TheReactivityoftheDisulphideBondofPurifiedProteinsinRelationshiptoPrimaryStructure[J].Biochem.J.,1967,104(2):473~479.[11]RennenbergH.DifferencesintheUseofCysteineandGlutath-ioneasSulfurSourceinPhotoheterotrophicTobaccoSuspensionCultures[J].Z.PflanzenPhysio.l,1981,(105):31~40.[12]RennenbergH,SchmitzK,BergmannL.Long-distanceTrans-portofSulfurinNicotianaTabacum[J].Planta,1979,147(1):57~62.[13]AndersonJV,ChevoneBI,HessJL.ReactiveOxygenSpeciesandAntioxdantsRelationshipinGreenCells[J].Physio.lPlan,t1992,100(2):224~233.[14],.[J].,1990,16(1):37.[15],,,.[J].,2009,44(1):93~97.[16]RauserWE.Phytochelatins[J].Ann.Rev.Biochem.,1990,(59):61~86.[17],,,.Cd2+)[J].,2005,20(3):50~53.[18],,,.[J].,2005,25(6):570~575.[19],,,.ABA[J].,2006,26(4):736~742.[20],,,.CO2[J].,2008,26(4):402~406.[21],,,.He-Ne[J].,2008,32(5):1002~1006.[22],,,.[J].,2005,21(4):58~62.[23],,,.[J].,1996,22(4):379~384.[24],,,.[J].,1996,38(9):735~742.[25],,.SODGRGSHAsA[J].,1995,37(10):776~780.[26],,,.)[J].,2007,(8):40~42.[27],,,.)[J].,2000,23(4):13~16.[28],.)H2O2[J].,2008,36(29):12575~12576.[29],,,.)[J].,2005,21(10):185~187.[30],,,.[J].,2003,22(5):508~511.[31],,,.[J].,2008,36(3):150~154.[32],.[J].,2007,16(6):164~169.[33],,.[J].,2001,27(2):109~113.(上接第117页)3.7,,,:[1].[J].,2007,(3):33~36.[2],.[J].,2006,(3):142~145.[3],,.[J].,2008,(4):244~246.[4].,[J].,2005,(1):9~11.[5],,,.[J].,2008,(5):944~948.[6],,,.[J].(),2009,(2):179~182.1214: