党参总酮体外抗氧化活性的研究

学号：071140121本科毕业论文党参总酮体外抗氧化活性的研究姓名：赵芬芳班级：071120121专业：生物工程指导教师：姜宁中国·恩施二O一五年五月Std.ID:071140121BACHELOR’STHESISOFHUBEIUNITroloxRSITYFORNATIONALITIESAntioxidantactivitiesoftotalflavonoidsfromradixcodonopsisFacultyName:SchoolofBiologicalScienceandtechnologyMajor:BiologicalengineeringStu-Name:ZhaofenfangMentor:JiangNingEnshiChina郑重声明本人郑重声明如下:1.本人所呈交的学士学位论文是在指导老师的指导下自己独立完成的。2.本所有点材料，实验和数据都是真实的3.本论文中引用他人已经发表的成果、数据、观点等，均已注明出处。4.除本文中已经注明引用的内容外，不包含任何其他已经发表或撰写过的科研成果。本声明的法律责任由本人承担。论文作者签名:日期:摘要目的：研究党参总黄酮的体外抗氧化活性。方法：以超氧阴离子自由基、、羟自由基、ABTS自由基、DMPD自由基、DPPH自由基和过氧化氢的清除能力，铁离子螯合力、总还原力、铁离子还原力为指标，测定党参总黄酮体外抗氧化活性，同时以EDTA-2Na、VC和Trolox作为阳性对照。结果：党参总黄酮体外抗氧化活性的结果表明，超氧阴离子自由基清除力为（11.79±0.8）μmolVC当量/mg,羟自由基清除力为（0.58±0.02）mmolVC当量/mg，ABTS自由基清除力为(0.35±0.1)μmolVC当量/μg、（0.04±0.01）μmolTrolox当量/μg，DMPD自由基清除力为（17.11±1.1）μmolVC当量/mg，DPPH自由基清除力为（14.71±1.02）μmolVC当量/mg、（6.87±0.5）mmolTrolox当量/mg，过氧化氢的清除力为（95.4±6.5）μmolVC当量/mg，铁离子螯合力为（10.48±0.8）μmolEDTA当量/mg，总还原力为（125.31±2.7）mmmolVC当量/mg、（96.71±1.3）μmolTrolox当量/mg，铁离子还原力为（0.31±0.01）μmolVC当量/mg、（6.59±0.6）μmolTrolox当量/mg。结论：党参总黄酮具有较强的体外抗氧化活性，该研究为党参总黄酮进一步开发提供理论依据。关键字：党参；总黄酮；体外抗氧化ABSTRACTObjective：ToinvestigatetheantioxidantactivitiesinvitrooftotalflavonoidsfromRadixcodonopsis.Method：Someantioxidantindexesinvitroincludingsuperoxideanionfreeradicals,hydroxylradicals,ABTS,DMPD,DPPHfreeradicalandhydrogenperoxidescavenging,ironionchelatingability,thetotalreducingpowerandferricionreducingpowerweredetermined,whileEDTA-2Na,VC,andTroloxwereusedaspositivecontrol.Result：InvitroantioxidantactivitiesoftotalflavonoidsfromRadixCodonopsisshowedthatsuperoxideanionradicalscavengingcapacity（11.79±0.80）μmolVCEquivalent/mg;hydroxylradicalscavengingcapacity（0.58±0.02）mmolVCEquivalent/mg;ABTSfreeradicalscavengingcapacity(0.35±0.10)μmolVCEquivalent/μg,（0.04±0.01）μmolTroloxEquivalent/μg;DMPDscavengingcapacity（17.11±1.10）μmolVCEquivalent/mg;DPPHfreeradicalscavengingcapacity（14.71±1.02）μmolVCEquivalent/mg,（6.87±0.50）mmolTroloxEquivalent/mg;hydrogenperoxidescavengingcapacity（95.4±6.50）μmolVCEquivalent/mg;ironionchelatingability（10.48±0.8）μmolEDTAEquivalent/mg;thetotalreducingpower（125.31±2.7）mmmolVCEquivalent/mg,（96.71±1.3）μmolTroloxEquivalent/mgandironionreduction（0.31±0.01）μmolVCEquivalent/mg,（6.59±0.60）μmolTroloxEquivalent/mg.Conclusion：TotalflavonoidsofRadixCodonopsisexhibitedstrongantioxidantactivity,thestudyprovidesatheoreticalbasisforitsfurtherdevelopment.Keywords：Radixcodonopsis;flavonoids;antioxidantactivities目录1.前言...................................................................................................................................................11.1党参的介绍............................................................................................................................11.1.1党参地理分布..............................................................................................................11.1.2党参的化学成分..........................................................................................................11.1.3党参的药理作用..........................................................................................................11.2黄酮类化合物的介绍.............................................................................................................21.2.1黄酮类化合物的分类..................................................................................................21.2.2黄酮类化合物的药理作用..........................................................................................21.3抗氧化活性............................................................................................................................31.3.1体外抗氧化活性评价方法..........................................................................................31.3.2体外抗氧化活性评价方法的原理..............................................................................31.4实验背景及意义.....................................................................................................................52.实验材料与方法...............................................................................................................................62.1.实验材料................................................................................................................................62.2实验试剂................................................................................................................................62.3实验仪器................................................................................................................................72.4实验方法................................................................................................................................72.4.1溶液样品的制备和试剂的配制..................................................................................72.4.2芦丁标准曲线的测定..................................................................................................92.4.3党参黄酮的提取与测定............................................................................................102.4.4党参总黄酮体外抗氧化活性的测定.......................................................................102.4.4.1超氧阴离