11株杂交竹枯萎病病菌生物学特性研究

11梁祖昂1,蔡吉苗2,刘先宝2,李超萍2,时涛2,黄贵修2*(1.,530000;2.,,571737)[目的]对11株杂交竹枯萎病病菌进行基础生物学特性研究[方法]以11株分离自广西华劲竹林发展有限公司枯萎病发病严重竹林基地的杂交竹枯萎病病菌为材料,进行最适生长条件与孢子萌发最适条件的确认及致死温度的测定[结果]11株菌株的最适生长培养基和光照条件各不相同,最适生长温度为24~28,最适pH值在7.0~11.0,乳糖和山梨醇为最佳碳源,酵母浸膏为最佳氮源分生孢子萌发的最适温度为24~30左右,最佳碳源为麦芽糖乳糖蔗糖,最佳氮源为酵母浸膏牛肉浸膏分生孢子的致死温度为50(10min)[结论]该研究为杂交竹枯萎病的防治奠定了良好的理论基础杂交竹;枯萎病;生物学特性S763.1A0517-6611(2010)21-11190-04ResearchonBiologicalCharacteristicsof11StrainsofBlightGermsinHybridBambooLIANGZuangetal(GuangxiHuajinBambooDevelopmentCompanyLtd.,Nanning,Guangxi530000)Abstract[Objective]Theamiwastoresearchthebasicbiologicalcharacteristicsof11strainsofblightgermsinhybridbamboo.[Method]With11strainsofblightgermsisolatedfromhybridbamboofrombambooforestbasewithsevereblightofGuangxiHuajinBambooForestDevelopmentltd.asmateria,lthemostsuitableconditionsfortheirgrowthandsporegerminationwereconfirmedandtheirlethaltemperaturewasdetermined.[Result]Themostsuitablegrowthmediaandilluminationconditionsfor11strainsweredifferen,ttheirmostsuitablegrowthtemperatureandpHvalue,optmiumcarbonsourceandnitrogensourcewere24-28,7.0-11.0,lactoseandsorbito,landyeastextractresp.Themostsuitabletemperatureforconidiumgerminationwasaround24-30,theoptmiumcarbonsourcesweremaltose,lactoseandsucrose,theoptmiumnitrogensourceswereyeastextractandbeefextrac.tItslethaltemperaturewas50(10min).[Conclusion]Thisresearchlaidagoodtheoreticalfoundationforcontrollingblightinhybridbamboo.KeywordsHybridbamboo;Bligh;tBiologicalcharacteristics3(BambusapervaiabilisMcClure),(DendrocalamopsisdaiiKeng.f),[1],,,,,,,[2-3],,20067,,,,,,11,(Fusariumoxysporum),,11.11.1.111,11.1.2(PDA)(PSA)(CMA)(OA)(MA)(CA)Czapek1Table1StrainstestedandsamplingsiteBacteriaNo.SamplingsiteBacteriaNo.SamplingsiteBaFOGX17BaFOGX72BaFOGX2BaFOGX87BaFOGX37BaFOGX9BaFOGX4BaFOGX10BaFOGX5BaFOJXBaFOGX6![4]1.21.2.1广西华劲竹林发展有限公司横向科技合作攻关课题梁祖昂(1972-),男,广西南宁人,农艺师,从事植物保护研究*通讯作者,研究员,Emai:[e-mail]20100317PDA282,4mm9cm20ml,,PDAPSACMAOAMACA,5101520242628303337,pH4.05.06.07.08.09.010.011.012.0张彩丽卢瑶,JournalofAnhuiAgr.iSc.i2010,38(21):11190-11193,11328,(12h)3D-D-D-D-(Czapek,),7d,,,3,(mm/d)1.2.2105/ml,,100l,288h100l1.6%,,5101520242628303337408h,100l404550556010min1.6%,288h100,,322.12.1.12,BaFOGX8BaFOGX9;BaFOGX8MA9.9mm/d,OA14.7mm/d,4.8mm/d;BaFOGX9PDA9.1mm/d,CMA14.73mm/d,5.66mm/d92Table2Effectsofdifferentmediumonthegrowthrateofstrainstestedmm/dTreatmentBaFOGX1BaFOGX2BaFOGX3BaFOGX4BaFOGX5BaFOGX6BaFOGX7BaFOGX8BaFOGX9BaFOGX10BaFOJXCMA12.4ab10.8ab12.5ab10.1b13.1a12.6a12.3c14.3a14.7a12.4ab14.0aOA14.1a10.2b13.8ab13.3a10.3b12.6a12.8bc14.7a14.4a11.4c13.8aMA10.813b10.8ab12.0ab9.5b9.2b12.3ab13.1bc9.9c13.1b11.1c12.7bCA11.313ab12.7a11.5ab11.3ab10.6b12.6a13.9a14.5a12.5b11.6bc13.9aPDA11.3ab11.3ab11.2b10.2b10.6b13.4a13.5ab13.2b9.1c12.0bc14.1aPSA11.9ab10.1a14.1a11.1ab9.7b10.1b13.6ab13.1b9.3c13.2a12.3b:=0.05Note:Differentlowercasesinthesamecolumnstandfordifferenttreatmentsofbacteriahavesignificantdifferencesat=0.05.2.1.2PDA,24~28,,BaFOGX7BaFOGX8BaFOGX9BaFOGX1024,BaFOGX428,26(3)3Table3Effectsofdifferenttemperatureonthegrowthrateofstrainstestedmm/dBacteria5101520242628303337BaFOGX10g2.3f4.3e10.6c13.0b14.0a13.0b11.0c6.0d0gBaFOGX20h1.8g3.1f9.0d12.0b13.0a11.7bc11.0c5.7e0hBaFOGX30h1.6g2.4f10.8c13.2a13.6a11.4b10.0d6.0e0hBaFOGX40e1.9d3.1c8.6a8.8a9.0a9.3a9.0a4.4b0eBaFOGX50i2.0h2.7g9.0e11.8d13.8a13.2b12.0c6.2f0iBaFOGX60h2.0g3.6f9.6d10.2cd12.0a11.0b10.4bc7.6e0hBaFOGX70g3.2f4.8e8.5d13.3a12.9a11.6b10.5c4.7e0gBaFOGX80h2.9g6.8e10.6d14.2a13.5b11.9c10.2d5.3f0hBaFOGX90g2.9f5.8d8.1c10.3a9.0b8.9b5.3d3.7e0gBaFOGX100f2.0e3.8d6.7c10.6a9.8a10.2a8.0b4.1d0fBaFOJX0g1.9f5.1e10.1c14.3a14.8a12.7b10.4c5.6d0g2.1.3pHpH4.0~7.0PDA,,pH,7.0~11.0pH,(4)2.1.4,,BaFOGX1BaFOGX9,BaFOGX4,(5)2.1.56,,,,BaFOGX3BaFOJX,BaFOGX8BaFOGX10,7,11BaFOGX9,,2.22.2.11,,,BaFOJX10,10~4024~301119138卷21期梁祖昂等11株杂交竹枯萎病病菌生物学特性研究4pHTable4EffectsofdifferentpHvalueonthegrowthrateofstrainstestedmm/dBacteria4.05.06.07.08.09.010.011.012.0BaFOGX18.6e9.9d10.7cd12.2ab12.3ab13.1a11.5bc12.5ab11.6bcBaFOGX27.3d10.4c11.3bc11.9ab12.7a11.6b11.3bc11.3bc12.7cBaFOGX38.4e11.1d12.6abc13.4a12.9ab12.7abc12.8abc12.1bcd11.8cdBaFOGX47.6e10.3d10.1d12.2bc12.9ab13.5a12.9ab12.3bc11.7cBaFOGX56.7e8.6d10.2c11.0bc11.4b11.4b12.6a11.6b9.3dBaFOGX68.3d11.6c12.0bc12.4abc12.8ab13.3a12.8ab12.2bc11.7cBaFOGX78.7b12.3a12.4a12.2a12.5a12.6a12.5a12.47a12.5aBaFOGX810.8e13.0d13.3cd13.6abc13.7abc13.3bcd13.0d13.8a13.7abBaFOGX98.5e8.9de9.5cd10.0c9.9c11.7b14.0a13.8a13.6aBaFOGX1011.7de13.2ab12.1cde13.4a13.0abc13.3ab12.4bcd12.7abc11.5eBaFOJX11.5d14.4a14.1ab14.8a14.3a14.0ab14.1ab13.3bc12.7c5Table5Effectsofdifferentlightingconditionsonthegrowthrateofstrainstestedmm/dTreatmentBaFOGX1BaFOGX2BaFOGX3BaFOGX4BaFOGX5BaFOGX6BaFOGX7BaFOGX8BaFOGX9BaFOGX10BaFOJX12.6b12.87a14.53a11.53a13.85a13.6a12.53a13.47a10.6a13.53a14.87a11.5c11.08b13.6b11.72a12.76b11.46c11.1c11.27c10.5a11.73c12.0b13.05a11.23b12.55c10.5b12.62b12.3b11.67b12.27b10.73a12.27b12.4b6Table6Effectsofdifferentcarbonsourcesonthegrowthrateofstrainstestedmm/dBacteriaDDfructoseDDsucroseDDglucoseSorbitolLactoseMaltoseDDxyloseCKBaFOGX111.5f11.2g11.7e12.1bc12.