pPICZαA质粒图谱

pPICZaa载体基本信息出品公司:Invitrogen载体名称:pPICZalphaA,pPICZαA,pPICZalphaA,pPICZαA,pPICZaA,pPICZa-A质粒类型:毕赤酵母蛋白表达载体表达水平:高拷贝启动子:AOX1克隆方法:多克隆位点，限制性内切酶载体大小:3593bp5'测序引物及序列:5´AOX1:5´-GACTGGTTCCAATTGACAAGC-3´3'测序引物及序列:3´AOX1:5´-GCAAATGGCATTCTGACATCC-3´载体标签:C-Myc,C-His载体抗性:Zeocin博来霉素筛选标记:HIS4备注:插入基因是必需包含起始密码子ATG。产品目录号:V195-20稳定性:稳定Stable组成型:组成型Constitutive病毒/非病毒:非病毒pPICZaa载体质粒图谱和多克隆位点信息pPICZaa载体简介pPICZαA，B和C是3.6kb的毕赤酵母蛋白分泌表达载体。表达的重组蛋白是融合蛋白，含有一个N-端多肽，编码酿酒酵母（Saccharomycescerevisiae）α-因子分泌信号。载体能够在毕赤酵母中利用甲醇诱导的高水平的表达目的蛋白，并且可以用在任何毕赤酵母中，包括X33，GS115菌株，SMD1168H，KM71H。pPICZαA，B和C系列载体包含以下元素：•5'端含有AOX1启动子的严格调控，利用甲醇诱导表达任何感兴趣的基因（Ellis等，1985;Koutz等人，1989;tschopp等人，1987A）。•α-因子分泌信号能够分泌性表达目的蛋白。•Zeocin抗性基因在大肠杆菌(Escherichiacoli)和毕赤酵母都能用于筛选（Baron等人,1992;Drocourt等人，1990）。•C-端含Myc和His标签，可以用于检测和纯化重组蛋白。•pPICZA,B,C三种读码框使得可以将基因克隆入载体而不发生任何移码突变。pPICZaa载体序列LOCUSpPICZα\A3593bpDNAcircularSYN2-SEP-2003SOURCEORGANISMCOMMENTThisfileiscreatedbyVectorNTI|biofeng.com|FEATURESLocation/Qualifierssource1..3593/invitrogen=25/vntifkey=98primercomplement(1423..1443)/invitrogen=1880000/vntifkey=27/label=3'\AOX1\primerprimer855..875/invitrogen=1890000/vntifkey=27/label=5'\AOX1\primerCDS1320..1337/invitrogen=1010000/vntifkey=4/label=6xHisprimer1144..1164/invitrogen=1900000/vntifkey=27/label=alpha-factor\primerterminator1341..1682/invitrogen=2550000/vntifkey=43/label=AOX1\transcription\terminatorCDS1275..1304/invitrogen=1090000/vntifkey=4/label=c-myc\epitopeterminator2538..2855/invitrogen=2560000/vntifkey=43/label=CYC1\transcription\terminatorpromoter2095..2162/invitrogen=2340000/vntifkey=30/label=EM7\promotermisc_feature1208..1274/invitrogen=1460000/vntifkey=21/label=MCSrep_origincomplement(2866..3539)/invitrogen=2540000/vntifkey=33/label=pUC\originCDS941..1207/invitrogen=1280000/vntifkey=4/label=alpha-factor\signal\peptidepromoter1683..2094/invitrogen=2300000/vntifkey=29/label=TEF1\promoterCDS2163..2537/invitrogen=1370000/vntifkey=4/label=Zeo(R)promoter1..940/invitrogen=2160000/vntifkey=29/label=AOX1\promoterBASECOUNT892a886c801g1014tORIGIN1agatctaacatccaaagacgaaaggttgaatgaaacctttttgccatccgacatccacag61gtccattctcacacataagtgccaaacgcaacaggaggggatacactagcagcagaccgt121tgcaaacgcaggacctccactcctcttctcctcaacacccacttttgccatcgaaaaacc181agcccagttattgggcttgattggagctcgctcattccaattccttctattaggctacta241acaccatgactttattagcctgtctatcctggcccccctggcgaggttcatgtttgttta301tttccgaatgcaacaagctccgcattacacccgaacatcactccagatgagggctttctg361agtgtggggtcaaatagtttcatgttccccaaatggcccaaaactgacagtttaaacgct421gtcttggaacctaatatgacaaaagcgtgatctcatccaagatgaactaagtttggttcg481ttgaaatgctaacggccagttggtcaaaaagaaacttccaaaagtcggcataccgtttgt541cttgtttggtattgattgacgaatgctcaaaaataatctcattaatgcttagcgcagtct601ctctatcgcttctgaaccccggtgcacctgtgccgaaacgcaaatggggaaacacccgct661ttttggatgattatgcattgtctccacattgtatgcttccaagattctggtgggaatact721gctgatagcctaacgttcatgatcaaaatttaactgttctaacccctacttgacagcaat781atataaacagaaggaagctgccctgtcttaaacctttttttttatcatcattattagctt841actttcataattgcgactggttccaattgacaagcttttgattttaacgacttttaacga901caacttgagaagatcaaaaaacaactaattattcgaaacgatgagatttccttcaatttt961tactgctgttttattcgcagcatcctccgcattagctgctccagtcaacactacaacaga1021agatgaaacggcacaaattccggctgaagctgtcatcggttactcagatttagaagggga1081tttcgatgttgctgttttgccattttccaacagcacaaataacgggttattgtttataaa1141tactactattgccagcattgctgctaaagaagaaggggtatctctcgagaaaagagaggc1201tgaagctgaattcacgtggcccagccggccgtctcggatcggtacctcgagccgcggcgg1261ccgccagctttctagaacaaaaactcatctcagaagaggatctgaatagcgccgtcgacc1321atcatcatcatcatcattgagtttgtagccttagacatgactgttcctcagttcaagttg1381ggcacttacgagaagaccggtcttgctagattctaatcaagaggatgtcagaatgccatt1441tgcctgagagatgcaggcttcatttttgatacttttttatttgtaacctatatagtatag1501gattttttttgtcattttgtttcttctcgtacgagcttgctcctgatcagcctatctcgc1561agctgatgaatatcttgtggtaggggtttgggaaaatcattcgagtttgatgtttttctt1621ggtatttcccactcctcttcagagtacagaagattaagtgagaccttcgtttgtgcggat1681cccccacacaccatagcttcaaaatgtttctactccttttttactcttccagattttctc1741ggactccgcgcatcgccgtaccacttcaaaacacccaagcacagcatactaaattttccc1801tctttcttcctctagggtgtcgttaattacccgtactaaaggtttggaaaagaaaaaaga1861gaccgcctcgtttctttttcttcgtcgaaaaaggcaataaaaatttttatcacgtttctt1921tttcttgaaatttttttttttagtttttttctctttcagtgacctccattgatatttaag1981ttaataaacggtcttcaatttctcaagtttcagtttcatttttcttgttctattacaact2041ttttttacttcttgttcattagaaagaaagcatagcaatctaatctaaggggcggtgttg2101acaattaatcatcggcatagtatatcggcatagtataatacgacaaggtgaggaactaaa2161ccatggccaagttgaccagtgccgttccggtgctcaccgcgcgcgacgtcgccggagcgg2221tcgagttctggaccgaccggctcgggttctcccgggacttcgtggaggacgacttcgccg2281gtgtggtccgggacgacgtgaccctgttcatcagcgcggtccaggaccaggtggtgccgg2341acaacaccctggcctgggtgtgggtgcgcggcctggacgagctgtacgccgagtggtcgg2401aggtcgtgtccacgaacttccgggacgcctccgggccggccatgaccgagatcggcgagc2461agccgtgggggcgggagttcgccctgcgcgacccggccggcaactgcgtgcacttcgtgg2521ccgaggagcaggactgacacgtccgacggcggcccacgggtcccaggcctcggagatccg2581tcccccttttcctttgtcgatatcatgtaattagttatgtcacgcttacattcacgccct2641ccccccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccct2701atttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttctt2761ttttttctgtacaga