奈米技术平台建构与生物晶片技术整合应用分项计划...

2Developmentandapplicationonthenanobio-chiptechnologyforenvironmentalinspection920411921210(02)8662-5933()0928899487152(02)[e-mail]481.2.2-1//1nm491nm100nm1922.2A.B.C.D.A.:50B.C.2a.b.D.2.3A.B.8003,00051/C.3CK-123.200252ICDRAMDNADNA4.5371855.Biochipsgenechipproteinchipcellchiptissuechip54humanchipSensingChipMicro-FluidicBiochipelectrophresischipsamplepreparationchipcombinatorychemistryonachipDNAmicroarrayUniversalchipSequencingbyhybridization6-8mersAffymetrix+HyseqStandardizedchipWhole-genome-on-a-chipN-mersCustom-madechip6.(environmentalbiotechnology)(bioremediation):(1)A.55()B.(POP)(PAH)(H2S)C.(BAC)(a)COD(b)(87)Lab.-scalescale-uppilot-plant56pH8711CODA/OA/O(2)5716SrDNAFluorescenceIn-SituHybridization,FISHDNArRNAcloningDNA(PCR)DNAcloningRestrictionEnzyme(DenatureGradientGelElectrophoresis,DGGE)DNACommunityFingerPrintPCRPCRcloningDNA1587.---GeneProbeRT-PCR16SrRNA-basedDAN-chip19A.toxicantsCdCl2BaPTCEDNA-chipPCR2426B./ontime/in-sitedenitrificationNO2-→N2PCRPrimernirKandnirSrRNA-targetprobesRT-PCR811C.waterbloomstoxin-producingbacteriacyanobacteriaGeneExpressionRT-PCR9D./MNALNAPLsDNAPLs/RT-PCR9E.bioremediationHg+2肽1416F.GEM59GEMsGEBs12,13G.PMNPre-ManufactureNoticeGEMs14H.biphenyldioxygenasesBPDoxsnaphthalenedoxsdioxindoxsinvitroDNA-shuffling19,20I./greenchemistryBDSDNA-shuffling19,20J.DNA-chipPCRGenotoxicEffects2227K.biosensorwhole-cellbiosensorDNA-bindingassays28L.2930M.313560(1)(2)(3)8.(1)61(2-1)22-162*:2(2)()::A.PVA(polyvinylalcohol,PVA)(alginate)PVAPSB(PermeableSupportBioreactor)MFSB(MembraneFeedingSubstrateBioreactor)PSBMFSBPSBMFSB63CODB.COD75%~80%45%2.5g/m2/d10g/m2/dasCaCO3pH3.67~14.68g/m2/d2.0~5.0g/m2/dHRT2240%7:372CODF99.3%4:696CODF99.1%9672HRT750%4600mL/min2.8mg/LCODF92%64ORCA/OCOD1,5002,000mg/L7501,000mg/L(1)HRT=3.0,9.0,12.0dHRT21(2)HRT2.0,6.0,8.0d1.0,3.0,4.0d(3)=0.330.720.120.29mg-COD/mg-CODd65(4)=0.134.710.471.93mg-COD/mg-CODd(5)=00.22.04.0mg/L(6)MLSS=3000450030004000mg/L(7)pH6.08.0quinonePCBspHC/NC/PquinonePCB1.0ppm200400ppbpH9.07.05.0PCBpH9.07.05.0PCBPCBUbiquononeMenaquinone11(BAC)(a)COD(b)(87)Lab.-scalescale-uppilot-plantpH668711CODA/OA/O(3)67681.2.3.1.2.693.4.(92)(93)DNA70(94)(95)7116SrDNA16SrRNAFISHDGGEPCRFISH16SrRNAFISHDGGEDNADNA16SrDNADGGEPCRDNA(Primer)dNTP(polymerase)DNA2DNADNAPCRPCRPCR721.(1)a.73b.4-191924-188899091921501401221048415141414122.3.a.4-2744-266223413334243b.75/3/3()3/3()c.()4.a.b.765.a.1.2.3.4.b.1.2.773.c.1.2.3.a.1.2.3.b.1.2.6.(1)92(2)9378(3)94791.1-34-67-910-122.801.2.242.56-118006-26-46-1()1()6-212,280,0002,280,0002,280,0002()()145,00045,00045,0003()1100,000100,000100,0002,425,000816-2()13,000X230*1230DNA20,000X260*1260-18C50*150PCR230*1230DNA40*14050*2100180*1180DNA70*2140(shaker)90*1905L210*1210UV180*2360DNAUVcross-linker50*150Fractioncollector140*114090*2180DNA40*1402,3006-32,300,0002,000,000300,0006-23,400,0003,400,00006-411,600,0008,600,0003,000,0006-517,300,00014,000,0003,000,000826-413,000X250*1250DNA20,000X300*13004C50*2100-18C50*2100PCR300*1300DNA40*28050*2100200*1200DNA200*120070*2140(shaker)100*22005L250*1250UV200*2400DNAUVcross-linker50*2100Fractioncollector150*2300100*3300DNA40*2803,400836-52,500*12,5003,000*13,000DNA600600DNA400400DNA1,000*11,000DNAFISH1,400*11,400D-code600*1600DGGETGGESSCPUV400*1400ELISAreader600*1600-80500*1500700*1700700*1700800*1800DNA13200841.2.3.4.1.2.3.4.85