沙冬青细胞与分子生物学研究进展

2010,11(6)793797JournalofPlantGeneticResources沙冬青细胞与分子生物学研究进展林清芳,王茅雁,刘佳杰,赵欢欢,王存芳(,010018):沙冬青是我国西北荒漠区唯一的常绿阔叶灌木,具有极强的抗逆性和特殊的医疗保健作用本文从细胞学与遗传多样性抗逆性分子基础与基因克隆和药用成分及作用机制方面对沙冬青的研究进展进行了综述,并提出了今后的重点研究方向:沙冬青;细胞学与遗传多样性;抗逆基因;药用成分ResearchProgressofCellandMolecularBiologyofAmmopiptanthusLINQingfang,WANGMaoyan,LIUJiajie,ZHAOHuanhuan,WANGCunfang(CollegeofLifeScience,InnerMongoliaAgriculturalUniversity,Huhhot010018)Abstract:AsanonlyevergreenbroadleafshrubinthenorthwestdesertofChina,Ammopiptanthusmongolicusshowsverystrongresistancetoenvironmentalstressesandspecialmedicalandhealthcarefunctions.Inthisreview,researchprogressinthecytologyandgeneticdiversity,thestressresistancemolecularbasesandgenecloning,themedicinalcomponentsandtheirfunctionalmechanismsoftheplantwerereviewed,andthefurtherresearchaspectsweresuggested.Keywords:Ammopiptanthus;Cytologyandgeneticdiversity;Stressresistancegene;Medicinalcomponents:20100120:20100728:(30960158);(K32227);(2009zx08009019B):,Emai:lwokeyi1234@163com:,,Emai:lwangmaoyan@163com[Ammopiptanthusmongolicus(Maxim)Cheng.f](Ammopiptanthus),(A.mongolicus)()(A.nanus)(),,[12],,,[1,3]2080,,,(antifreezeproteins,AFPs),,12060,,2n=20[45],2n=16[6],2n=18[713],11,X=9,2n=2x=18,[1415]2A[1213],:K(2n)=18=10m+4m(SAT)+2sm+2s,t711,K(2n)=18=8m+2m(SAT)+2sm+2sm(SAT)+4sm(SC),54[13]2n=2x=18=8m(4SAT)+8sm+2s,t2A,441;2n=2x=18=6m(2SAT)+10sm(2SAT)+2s,t3A,351,[14]Stebbins[16],,[14]10,DNA,,,,[14,17],[18],,[19]68(),,,,Ge[20]ISSR,,,DNA63%,,,,,,,,2210,0,[2122]2090,,,1969,[23],,AFGP(antifreezeglycoprotein)[24],,4,(hysteresis),,,,,,(thermalhysteresisproteins,THPs),,(thermalhysteresisactivity,THA)[2426],[2729]1992[27],[2931],1994,[29]7946:,457KD812KD,,045(10mg/ml)046(8mg/ml)3[32]40kD50kD,09(20mg/ml),N20(SDDLSFTFNKFVPCQTDILF)[33],035(5mg/ml),[3435],,,[3639],,,[40],Fei[41],,,,4015~048(20mg/ml)[42],284050kD[33,35,37],,,,,[43],,,,,,,,2.2,Liu[44-46],,(lateembriogenesisabundantprotein,LEA)(proteaseinhibitor,PI)ATPase1433,AmLEAAmPI,,AmPI,[47]Cao[48]cDNAAFLPReverseNorthern,,AmEBP1,,,AmGolS,,AmGolSABA,[49],AmGolS,[49]CBFs(Crepeatbindingfactors),CBFAmGS,,[5051]2.3CBLs(calcineurinBlikeproteins)Ca2+,[52][53]RACE,AmCBL1,79511,,AmCBL1[54][55]EST,5,360uniEST,313,48,,[56],,SMARTcDNA,,,3,,[3],,,,[3,57][58],,[58-59],[58],(),[58]JA1HepG2G1,,,[60],,,,,,[57-58],,,,,,,,,,,,[1],,.[J].,2000,13(5):6771[2],,,.[J].,2001,37(1):5257[3],,,.[J].,2008,33(19):22042206[4]GoldblattP.Indextoplantchromosomenumbersfor19751978[J].MissuuriBotGard,1981:220[5]GoldblattP.Indextoplantchromosomenumbersfor19821983[J].MissuuriBotGard,1985:173[6],.[J].,1988(3):29[7]FedorovAA.Chromosomenumbersoffloweringplant[J].Leningrad:AcadSciUSSR,KomarovBotanicalInstitute,1969:314[8]BoczantsevaVV.ChromosomenumbersoftwoshrubsfromthefamilyLeguminosae[J].BotZh,1976,61:1441[9],.[J].,1987(1):2829[10],,.[J].,1988(1):5863[11],.[J].,1990,28(2):133135[12]ChenCJ,ZhuXY,YuanYM.CytologicalstudiesonthetribeThermopsideae(Fabaceae)[C]//Reportonkaryotypesofelevenspeciesoffourgenera.Cathaya,1992,4:103116[13],.[J].,1993,35(4):314317[14],,.[J].,1996,15(4):4047[15],,,.[J].,2003,11(2):166168[16]StebbinsGL.Chromosomalevolutioninhigherplants[M].7966:London:EdwardArnoldLtd,1971[17],.[J].,1995,12(1):5354[18],.[J].,1989,27(1):5357[19],,.[J].,2005,23(2):131137[20]GeXJ,YuY,YuanYM,eta.lGeneticdiversityandgeographicdifferentiationinendangeredAmmopiptanthus(Leguminosae)populationsindesertregionsofnorthwestChinaasrevealedbyISSRanalysis[J].AnnBot,2005,95(5):843851[21]BurkeMJ,GustaLV,QuammeHA.Freezingandinjuryinplants[J].AnnRevPlantPhysio,l1976,27:507528[22],,.[J].,2006(6):5052[23],,,.[J].,2004,26(4):482487[24]DeVriesAL.Antifreezeglycopeptidesandpeptides:interactionwithiceandwater[J].MethodsinEnzymology,1986,127:293303[25]KnightCA,DumanAG.Inhibitionofrecrystallizationoficebyinsectthermalhysteresisproteins:apossiblecryprotectiverole[J].Cryobiology,1986,23:256262[26],,,.[J].,1999,41(7):677683[27]GriffithM,AlaP,YangDSC,eta.lAntifreezeproteinproducedendogenouslyinwinterryeleaves[J].PlantPhysio,l1992,100:593596[28]DaviesPL,HewCL.Biochemistryoffishantifreezeproteins[J].FASEBJ,1990,4:24602468[29],,,.[J].,1994,36(8):649650[30]WorrallD,EliasL,AshfordD,eta.lAcarrotleucinerichrepeatproteinthatinhibitsicerecrystallization[J].Science,1998,282(2):115117[31],,,.[J].,2001,23(4):342345[32],,,.[J].,1999,41(9):967971[33],,,.[J].,1999,41(8):837841[34],,,.[J].,2000,45(20):21852189[35],,,.[J].,2003,1(12):4545[36]WangW,WeiL.PurificationofboilingsolubleantifreezeproteinfromthelegumeAmmopiptanthusmongolicus[J].PrepBiochemBiotech,200333(1):6780[37]WangW,WeiL,WangG.MultisteppurificationofanantifreezeproteinfromAmmopiptanthusmongolicusbychromatographicandelectrophoreticmethods[J].JChromatogrSc,i2003,41(9):48993[38].[D].:,2004[39],,.[J].(),2007,44(4):912917[40],,,.DSC[J].,2001,17(1):6669[41]FeiYB,CaoPX,GaoSQ,eta.lPurificationandstructureanalysisofantifreezeproteinsfromAmmopiptanthusmongolicus[J].PrepBiochemBiotechno,l2008,38(2):172183[42],,,.[J].,2007,25(5):531534[43]YunBF,PengXC,SuQG,eta.lDiscoveryofaHomologofSiderophilininaPlant[J].JournalofIntegrativePlant