生物活性硼硅酸盐多孔支架的体外活性及细胞毒性

2009年第54卷第4期:463~468SCIENCEINCHINAPRESS,,,,,旵*,,,,200092;DepartmentofMaterialsScienceandEngineering,UniversityofMissouriScienceandTechnology,MissouriMO65409,USA;,200092;,200011*,E-mail:[e-mail],[e-mail]2008-11-14,2009-01-05(:05DJ14006)(:084419005000)(R2O-RO-B2O3-SiO2-P2O5),,4,.,,73%~80%,200~300μm,.0.02mol/LK2HPO4,,,D-Alk-2B,D-Alk-3B7h,,,.,,,,D-Alk-3B,(1:8),.,,.MTT.,.,,[1].,,,,,.,[2,3].,,,,45S5(Bioglass®),[4~6].,SiO2,,,[7,8].,.,,,(SBF,stimulatedbodyfluid),[9,10].,,,期,MTT,.11.114R2O-30RO-8xB2O3-(54−18x)SiO2-2P2O5,x=0,1,2,3,D-Alk-0B,D-Alk-1B,D-Alk-2BD-Alk-3B(0B,1B,2B3B).,1100~130050~60min,,50μm.(),(ethyl-cellulose,EC,).ECQM-ISP,3h,.(20/5cm,),,.,24h,6012h.,,1/min460,1h,3/min560~600,60~90min,,[11].1.2,0.02mol/LK2HPO4(K2HPO4,,),pH7.00,SBF,,,,K2HPO4PO43−SBF20,PO43−,.,,.K2HPO4,K2HPO4,37,pH,,pH.X(XRD,D/max2550V,Rigaku,Cu-Kα,Japan);(SEM,HitachiS-4700,Japan).BOptima2100DV(ICP-OES,Perlin-ElmerCo.USA).1.3MTTMTT,().ISO10993-5,4.2g10mL.,1:1,1:2,1:4,1:8.,20μL5mg/mL(MTT)CO24h.MTT,200μLDMSO,5min,(Elx800,BIO-TEK,USA),490nm.MTT臜(formazan),DMSO.,(A),A.A,100%,(RGR,relativegrowthrate,%),RGR=AA×实验组的平均值阴性对照组的平均值100%.(1)1RGR6[12].1a)(%)()100075~99150~74225~4930~24405a)01;2;3,45,5cm,5mL6×104/mL2,CO2,1,3,7d,464,(S-2360N,).22.1,,K2HPO4,pH,pH,pH[9,13].4pH,1.14pH,pH,,pH.,pH,,,[14],,,.4,,pH,,,.,(Na2O+K2O)/B2O31,[BO4],[BO3],,[10].2,4XRD,,.,XRD,.,XRD,.,,,,.pH.,73%~80%,,3(a)(c),(2-Alk-2B),,(3(b)),200~300μm.,.,50%,27d,4XRD3D-Alk-2BSEM(a);(b)[15];(c)0.02mol/LK2HPO47d;(d)4652009年2月第54卷第4期200μm,[16].,.3(c)(d)K2HPO47d,2BSEM.(c),K2HPO4,,(3(d)),,,XRD,.,,Ca2+PO43−,HAP,.,[17].,,.2.2MTTMTTCMTT()臜,.MTT,.()MTT.10mL,3d,1:1,1:2,1:4,1:8,A,4,4MTT,;,64g/L.A,,A,.40B,1B,2B,3BA.4:,A,,,,A;0B()A,;0B,1B,2B,1B3dA0B,;,,,,1B0B.1B1:2,1:4,1:8,2B1:8,A0B,A..().2MTTA,(1)RGR.,,,0B,1B,0~1,RGR,1B;2B(1:1,1:2),2,,;3B2,.5(BMSCs)SEM,.5(a)2BSEM,4662(3d)(:)RGRD-Alk-0B-3d1:10.76895111:21.00608801:41.06968101:81.0427620D-Alk-1B-3d1:10.82517311:21.16503501:41.18091501:81.0880180D-Alk-2B-3d1:10.50489621:20.69314621:40.75428211:81.0280930D-Alk-3B-3d1:10.60512721:20.46209731:40.59166821:80.6479262467,,,,.,,,.3BSEM,,.MTT,2B2,,;3B,RGR2,..,,,.,[18,19].21~24h,,80%~90%[20].,,.,.5SEM(a)D-Alk-2B;(b)D-Alk-3B3(),.K2HPO4,,HAP,;HAP,;HAP,.()4,.HAP,pH,,,,.,.()4,D-Alk-0B,D-Alk-1B;D-Alk-2B2,;D-Alk-3B..1LangerR,VacantiJP.Tissueengineering.Science,1993,260:920—926[doi]2HolyCE,ShoichetMS,DaviJE.Engineeringthree-dimensionalbonetissueinvitrousingbiodegradablescaffolds:Investigationini-tialcell-seedingdensityandcultureperiod.JBiomedMaterRes,2000,51:367—3822009年2月第54卷第4期3HenchLL.Bioceramics.JAmCeramSoc,1998,81(7):1705—17284WenL,MohamedNR,DelbertED.Bioactiveborateglassscaffoldforbonetissueengineering.JNon-crystSolids,2008,354:1690—1696[doi]5HenchLL.Sol-gelmethodforbioceramicapplications.CurrOpinSolidStateMaterSci,1997,2:604—610[doi]6RezwanK,ChenQZ,BlakerJJ,etal.Biodegradableandbioactiveporouspolymer/inorganiccompositescaffoldsforbonetissueengineering.Biomaterials,2006,27:3413—3431[doi]7HamadoucheM,MeunierA,GreenspanDC,etal.Long-terminvivobioactivityanddegradabilityofbulksol-gelbioactiveglasses.JBiomedMaterRes,2001,54:560—566[doi]8和峰,刘昌胜.骨修复用生物玻璃研究进展.玻璃与搪瓷,2004,32(5):46—509YaoAH,WangDP,HuangWH,etal.Invitrobioactivecharacteristicsofborate-basedglasseswithcontrollabledegradationbe-havior.JAmCeramSoc,2007,90(1):303—306[doi]10HuangWH,DayDE,KittiratanapiboonK,etal.Kineticsandmechanismsoftheconversionofsilicate(45S5),borate,andborosili-categlassestohydroxyapatiteindilutephosphoatesolutions.JMaterSciMaterMed,2006,17(7):583—596[doi]11LiuX,HuangWH,FuHL,etal.Bioactiveborosilicateglassscaffolds:Improvementonthestrengthofglass-basedscaffoldsfortis-sueengineering.JMaterSci:MaterMed,2008-9-21,[doi]12韩彦峰,李渊真,马辰春,等.五种无机载银抗菌剂的体外细胞毒性比较.中国组织工程研究与临床康复,2008,12(27):5287—529013姚爱华,王德平,FuQiang.可控降解性能的生物活性比例的制备及其生物活性研究.科学通报,2006,51(21):2562—256514宁佳,王德平,黄文旵,等.硅酸盐生物玻璃的制备及其体外生物活性和降解性.硅酸盐学报,2006,34(11):1326133015GibsonLJ.Themechanicalbehaviorofcancellousbone.JBiomech,1985,18:317—328[doi]16DaculsiG,PassutiN.Effectofthemacroporosityforosseoussubstitutionofcalciumphosphateceramics.Biomaterials,1990,11(1):86—8717KokuboTJ.Surfacechemistryofbioactiveglass-ceramics.Non-CrystSolids,1990,120:138—151[doi]18BenderdourM,Bui-VanT,DickoA,etal.Invivoandvitroeffectsofboronandboronatedcompounds.JTraceElemMedBiol,1998,12(1):2—719ForrestHN.Boroninhumanandanimalnutrition.Plantsoil,1997,193:199—208[doi]20刘平,胡伟.硼的生物效应及健康影响研究进展.世界科技研究与进展,2003,25(6):6—11·书讯·::978-7-03-022917-5:20091:95,...,..468