芒果炭疽病拮抗菌J_15的鉴定及其生物学特性研究

J-151，2(1.，266109；2.，266109):J-15，（Bacilluscereus）。14～45℃，38℃；pH5.0～9.0，pH6.5；。2.0%NaCl。。：；；；；：S436.67：A：1004-874X(2010)07-0101-04StudyonidentificationandbiologicalcharactersofantagonisticbacteriumJ-15RENJian-guo1,WANGJun-li2（1.CollegeofAgronomyandPlantProtection,QingdaoAgriculturalUniversity,Qingdao266109,China;2.CollegeofResourcesandEnvironment,QingdaoAgriculturalUniversity,Qingdao266109,China）Abstract:TheantagonisticbacteriumJ-15againstmangoanthracnosewasindentifiedasBacilluscereusbyculturalproperties,morphologycharacteristicsandphysiologicalandbiochemicalassay.ThestudyonthebiologicalcharacteristicsshowedthatthetemperatureandpHforJ-15growingeffectivelywas14～45℃andpH5.0～9.0accordingly,andtheoptimaloneswere38℃andpH6.5,respectively;thebestutilizationsforcarbon,nitrogenandaminoacidwereglucose,beefextractandasparagine,respectively;J-15grewwellundertheosmoticpressureof2.0%NaCl.Keywords:mangoanthracnose;antagonisticbacterium;physiologyandbiochemistry;idenitification;biology〔(Colletotrichumgloeosporioides(Penz.)Sacc.)〕。、、、，，、，，。，[1-2]，。，J-15[3]。。11.1J-15。1.21.2.1J-153～4d，，，，，。1.2.2J-15(3.0g，10g，5.0g，1000mL,18.0g)48h，。[4]：（）；()，KOH；（）；（）；（）。1.2.3J-15[5][6]，：、、(MR)、3-(V.P.)、、、、、、、、、、pH5.7sabouraud、、。1.31.3.1109CFU/mL1mL9mL（pH7.0），14、16、23、29、30、35、38、45℃（250r/min），1mL，3，24h625nm。1.3.2pH100μL（）pH5.0、6.0、6.5、7.0、7.5、8.0、9.0(9mL)，30℃250r/min，100μL，3，24h625nm。1.3.3[7]，0.2%(NH4)2SO40.05%NaH2PO40.05%K2HPO40.02%MgSO4·7H2O0.01%CaCl2·2H2O。：(1%)、(1%)、(1%)、(1%)、(0.5%)(0.5%)。1mL(1mL)，1.3.1，48h625nm。：2010-01-08：（1973-），，，E-mail:[e-mail]201071011.3.41.3.3，1%0.2%。：(0.5%)(0.1%)(0.2%)(0.3%)(0.1%)。1.3.1，24h625nm。1.3.5[7]，0.2%(NH4)2SO4、1%、0.05%NaH2PO4、0.05%K2HPO4、0.02%MgSO4·7H2O、0.01%CaCl2·2H2O。：，0.5%。1.3.1，24h625nm。1.3.6，0.5%、2.0%、5.0%、7.0%、10.0%，、、1.3.1，36h625nm。1.3.7，、、1.3.1，12h625nm。22.1J-1536h：，，，，，，。：，；；，；，。J-151。1[6]，(Bacilluscereus)。2.22.2.1J-15，24h，，1。1，(14~45℃)，38℃。2.2.2pHJ-1524h，，2。2，pH5.0～9.0，（pH6.5）。2.2.3J-1548h，，3。3，，＞＞＞。2.2.4J-1524h，，4。4，，。2.2.5J-1524h，，5。5，，，1J-15OD℃2pHJ-15ODpH3J-15OD+－－－+－－+++－－++－－D-L-D-D-D-5℃10℃30℃40℃50℃55℃2%5%7%10%+－－++++++－+++++MRV.P.pH5.7sabouraud1J-15102＞＞＞＞＞＞，，。，。2.2.6J-1536h，，6。6，，，0.5%~5.0%。2.0%。2.2.7J-1535℃，12h，7。7，12～36h，；36～84h，；84～96h；96～120h，；，。3，，，。，Bacillussubtilis[8-10]、B.licheniformis[10]，B.cereus[10]、Pseudomonasfluorescens[8,11-12]、Pichiaohmeri[8]、Sporobolomycessp.[8]、Brevundimonasdiminuta[13]、Stenotrophomonasmaltophilia[13]，Enterobacteriaceae[13]、Candidamembranifaciens[13]、[8,14]、Trichodermharzianum[10,12]、[15]，、。J-15B.cereus，，J-15，[3]，Benhamou[16]BacilluspumilusstrainSE34，，，。J-15(B.cereus)，、。，。Mandrik[17]Bacillussubtilis2(PseudomonassyringaeXanthomonasphaseoli)3(Colletotrichumlupini,Botrytiscinerea,Pleiochaetasetosa)，、C/NB.subtilis，、、，(NH4)2SO4+(1.0+0.025g/L)，C/N〔(NH4)2SO4：〕20。Havenga[18]34、20B.subtilis(Colletotrichumgloeosporioides)，、、B.subtilis，Colletotrichumgloeosporioides；，B.subtilis，，，。J-15(B.cereus，32.9%)、：、、4J-15OD5J-15OD6J-15NaCl(%)OD7J-15（h）OD103：[1].[J].,2000(79)：60-61.[2].[M].：,1998.[3],,，.[J].,2007,26(2):174-177.[4].[M].：,1994.[5],,.13[J].,2009,24(2):307-311.123456789（μg/mL）6.9110.7213.3927.5040.5066.00101.25366.67435.6155.567.279.0921.4030.0044.0056.25333.33b333.3345.888.0010.0025.00b36.00b48.8962.30400.00a357.1436.258.8911.1130.00a45.00a58.67b75.00500.00384.6226.67b10.00b12.50b32.5060.0073.33a90.00b666.67416.67b17.14a11.43a14.28a50.0090.0088.00112.50a1000.00454.55a··（μg/mL）1：aC-，bC+。13.39μg/mL，，3，，。。，，366.67μg/mL。··，，，435.61μg/mL。（100），Mandrik[17]Havenga[18]，：，B.cereus，Mandrik[17]Havenga[18]Bacillussubtilis，Bacillussubtilis，()，2；，J-15、，，Mandrik[17]Havenga[18]。，，。，J-15(B.cereus)，，、、，，，。：[1],,.25％·[J].,2004,24(2):1-3,8.[2],,,.[J].,2000,21(4):68-74.[3],,,.[J].,2002,15(4):82-85.[4].[M].:,1994.[5],,,.[M].:,1993.[6]RE,WC.[M].:,1983.[7],.[J].,1991,21(1):15-19.[8]ChuangTY,AnnPJ.Biologicalcontrolofmangoanthracnose[J].PlantProtectionBulletin(Taipei),1997,39(3):227-240.[9],,.[J].,1998,19(3):21-27.[10]YENJITP,INTANOOW,CHAMSWARNGC,etal.UseofpromisingbacterialstrainsforcontrollingAnthracnoseonleafandfruitofmangocausedbyColletotrichumgloeosporioides[J].WalailakJSci＆Tech,2004,1(2):56-69.[11]KoomenI,JeffriesP.Effectsofantagonisticmicroorganismsonthepost-harvestdevelopmentofColletotrichumgloeosporioidesonmango[J].PlantPathology,1993,42:230-237.[12]PrabakarK,RaguchanderT,SaravanakumarD,etal.ManagementofpostharvestdiseaseofmangoanthracnoseincitedbyColletotrichumgleosporioides[J].ArchivesofPhytopathologyandPlantProtection,2008,41(5):333-339.[13]KefialewY,AyalewA.Postharvestbiologicalcontrolofanthracnose(Colletotrichumgloeosporioides)onmango(Mangiferaindica)[J].PostharvestBiologyandTechnology，2008,50(1):8-11.[14]Patin軌o-VeraM,JiménezB,BalderasK,etal.Pilot-scaleproductionandliquidformulationofRhodotorulaminuta,apotentialbiocontrolagentofmangoanthracnose[J].JournalofAppliedMicrobiology,2005,99:540-550.[15]ZEN'ICHIM,TETSUYAT,SATOSHIT,etal.Studyonthebiologicalcontrolofmangoanthracnose:(II)controleffectofPenicilliumwaksmaniiZaleski(T-141)onmangofruitsandleaves[J].JapaneseJo