跨界融合选育生物脱硫催化剂

:2006210210;:2006212228:(1963-),,,()2005,:,KM2006100170011,2,1(1.,102249;2.),,,;,,,,,,:1,,,,,,,,:(Pseudomonassp.)(Rhodococcussp.)(Corynebacteriumsp.)(Brevibacteri2umsp.)(Gordonasp.)(No2cardiasp.)[13],1989Rhodococcusrhodochrous.IGTS81998,JeHwanChang(Gordinasp.CYKS1),(DBT),20,2000,MaghsoudiS(Corynebacteriumsp.P32C1),30min0.5mol/LDBT,IGTS8,P32C1(),DBT,,DBT,,DBT(DBTs)(BT)(BTs),,22.1,;9302.2;SH23600A;HZQ2C;LGR1024.2;DBT20076PETROLEUMPROCESSINGANDPETROCHEMICALS386©1994-2007ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[46]1(Penicillin,Pc);2(Nystatin,Nt);32.52.5.15g100mL,10mL80mL10mL(0.1mmol/L4,62),,120r/min28,48h(1);10mL,80mL10mL,120r/min28,48h;10(480h)[7]2.5.210,,30,,,42.5.3():100mL,,120r/min2824h,6000r/min15,0.5g/mL(0.5g1mL)2.72mmol/L5.44mmol/LDBT10mL,90mL0.5g/mL,,120r/min28,12h24h,DBTDBT,,[8]2.5.4DBT:254nm,90%,1mL/min,C1825,20L,DBT,DBT,DBTDBT[9]2.5.5(Bacillussubtilissubsp.),TL;:,4[10,11]2.5.6,,32,123,2.5.72.5.32.5.8,0.5g,100mL,,120r/min2824h;,0.5g,100mL,,;51(),2.5.9,85mL15mL93,,120r/min2812h,;,85mL,,120r/min2812h;3726.©1994-2007ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[12,13]15mL930,02.5.10930:600,800,700,150mL/min,150mL/min,,;,DBT,n8,DBTTp0.05;p0.05;p0.01DBT,p0.0133.1DBT,,DBT:y=7.055+0.04x,r=0.999912,800g/g,3,TL21,TL22,TL23,11DBTDBT()/mmolL-112hDBT/mmolL-1,%24hDBT/mmolL-1,%TL212.721.8233.831.5642.65TL222.722.2716.542.0225.73TL232.721.4247.791.0063.24TL215.444.0026.473.2340.63TL225.444.2721.504.0026.47TL235.443.4736.212.5652.941,3DBT,TL23;3,3:Bacillussubtilissubsp.3.222,,,(RH)2TL23(RH)1-++2+-+3--+-++-++-++:+;-3.3DBT33,RH21RH22DBT,,RH21RH22RH21RH22TL23,RH21RH22,TL233DBTDBT()/mmolL-112hDBT/mmolL-1,%24hDBT/mmolL-1,%TL232.721.4247.791.0063.242.722.7102.710RH212.721.5841.911.3550.36RH222.721.4646.321.1557.72TL235.443.4736.212.5652.945.445.4605.350RH215.443.5934.003.0543.93RH225.443.4636.392.5952.393.434545,RH21RH2212h324h3,,24h12h,82200738©1994-2007ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.()/mgL-112h/mgL-1,%24h/mgL-1,%RH21129.7531.1376.0122.4482.71RH22129.7552.2759.7131.6475.61:355()/mgL-112h/mgL-1,%24h/mgL-1,%RH21138.4635.7374.1927.5680.09RH22138.4672.0047.9939.6671.264(1),,3,(Bacillussubtilissubsp.)(2),,,,,(3),,,1KonishiJ,MaruhashiK.Residue345ofdibenzothiophene(DBT)sulfonemonooxygenaseisinvolvedinCSbondcleav2agespecificityofalkylatedDBTsulfones.BiotechnologyLet2ters,2003,25:119912022,,..,2005,24(9):100210053GuptaN,RoychoudhuryPK,DebJK.Biotechnologyofdesul2furizationofdiesel:prospectsandchallenges.ApplMicrobiolBiotechnol,2005,66:3563664,.().:,2003.2993415..:,1999.2052236,,..,2004,24(6):7307337..:,2004.2312388,,..,2005,3(2):139..:,2004.6497,18319210,,..,1997,27(4):343611TanakaY,MatsuiT,KonishiJ,etal.Biodesulfurizationofbenzo2thiopheneanddibenzothiophenebyanewlyisolatedRhodococ2cusstrain.ApplMicrobiolBiotechnol,2002,59:32532812,,..,2005,34(1):48949113CarolinaHdelO,AlmudenaA,VictoriaES,etal.ModelingtheproductionofaRhodococcuserythropolisIGTS8biocata2lystforDBTbiodesulfurization:Influenceofmediacomposition.EnzymeandMicrobialTechnology,2005,37(7):157166BREEDOFDESULFURATIONBIOCATALYSTWITHFUSIONOFSTRIDEKINGDOMShenQiying1,2,ZhaoSuoqi1(1.StateKeyLaboratoryofHeavyOilProcessing,ChinaUniversityofPetroleum,Beijing102249;2.DepartmentofChemicalEngineering,BeijingInstituteofPetro2chemicalTechnology)AbstractInordertoeffectivelyremovethesulfurcontainingorganiccompoundsfrompetroleumproducts,akindofmicroorganismstrainwasacclimatizedfromthecrudepollutedsoilwiththemixingofsomesulfurcontainingorganiccompounds.AfterselectionandseparationthestrainhavingcertaindesulfurationfunctionwasidentifiedasBacillussubtilissubsp.Usingsaidstrainandwhite2rotfungiasparentstrainsthroughprotoplastfusion,geneticstablefusinggameteshavingeffectivedesulfurationfunctionwasobtained.Theresultsofbio2desulfurationtestswithgasolineanddieselfuelshowedthatsaidfusionmicroorganismcouldbeusedtoremovethesulfurcontainingorganiccompoundsfrompetroleumproducts,andsaidstrainwasobservedwithrelativelyhighsulfurremovalrateandadaptabilitytowiderangeoftestconditions.KeyWords:protoplastfusion;biodesulfuration;catalyst926.©1994-2007ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.