黄麻分子生物学研究进展及展望

32：2010-05-27：（nycytx-19-E05）、（nyhyzx07018）：（1976-），，，，：。：（1948-），，。E-mail:[e-mail]，，350002：、、、，，。：；；；：S563.4：BDevelopmentandProspectofMolecularBiologyResearchonJuteTAOAi-fenQIJian-minLIXiao-zhenKeyLaboratoryofMinistryofEducationforGeneticsBreedingandMultipleUtilizationofCropsFujianAgricultureandForestryUniversityFuzhou350002ChinaAbstractThedevelopmentofmolecularbiologyresearchonjutewasreviewedinthispaperwhichincludedmolecularmarkersconstructionofgeneticlinkagemaptissueculturetransgenicbreedingandgenomicsanalysis.Thequestionsandprospectofmolecularbiologyresearchonjutewerealsodiscussed.Theideathatusingmodernmolecularbiologytechnologytospeedgermplasminnovationandbreednewjutevarietieswithgoodqualityhighyieldandgoodresistancewasputforward.KeywordsjutemolecularbiologydevelopmentprospectjuteTiliaceaeCorchorus。、、、、、、“”。、、、、、、[1]。。2050[2]。、、、、、、。。1DNA。DNA：1673-7636201004-0232-062010324PLANTFIBERSCIENCESINCHINA2324DNA、。DNARAPD、AFLP、SSR、ISSR、SRAP、STS、、、、、[3]。2001DNARAPD[4]。RAPDISSR0.33-0.460.9ISSR、RAPD[5-7]。HossainMB2002RAPD[8]HossainMB5RAPD8AFLP6[9]。A.Basu2004AFLPSSR4910AFLP305SSR2-4[10]。ReyazulR.MirSSR2、81SSRSSRSSR813[11]。ARoy2006RAPD、ISSR、STMS40[12]。SRAP173SRAP。。SRAP[13]。、。2。、、、。。Nishatsultana2006O-9897Acc.1805F28ISSR87.3cM334.8-52.9cM8.73cM[14]。20101。1185F226SRAP、21ISSR7RAPD2166.3cM10、123。17.33cM。———M4M5LG5M4807-22016.9cMM5M14E14-28026.4cM[15]。“”“”187F2。56SRAPPCR128SRAP、2522.2cM、19.25cM。、3LG2M18E7M12E1823332M17E10M12E9-1[16]。、、。3、、、、[17]。1964Islam[1]。A.Khatum。1978~1980。、、、、[18]。T.Saha70%[19]。AliM.Abbas、、NAAIAAIAABA[20]。Hossain[21]。R.H.SarkerCVL-1、CVE-3D-1543[22]。KMKHuda[23]。。41998HossaiAbmmD-154GUS[24]。M.GhoshbarRolC、PCR、Southern、Northern[25]。AbuAshfaqurSajibRT-PCR、PCRSouthern[26]。51999Herbaria.MAGenBankDNA[27]4GenBankNADHDNA[28]。AhmadS.IslamPSMMARTPBluescriplO-4CVL-1cDNA200511GenBank200DNA。tRNADNADNAGenBankDNA[29]。GenBankDNA400。6DNADNA35。30SRAP35DNADNA[30]。23447“179”。TCATris-HClTris-base/2-DE。ImageMaster2DPlatinumversion5.02-DE1791792-DE70029。88.1198020。。2008、、、。8.2、、。。1998。、、。99.1，40、。、、。“”。、、。9.2，23532、。2080。、70%、。12[1]。、。、、、。。：[1].[M]．2008.[2]http//baike.baidu.com/view/2461.htm[3]http//baike.baidu.com/view/660796.htm[4].DNARAPD[J].2001334-339.[5]．RAPD[J].200310926-932.[6]．ISSR[J].200391473-1477.[7]．RAPDISSR[J].200437122006-2011.[8]MohammadBelayatHossainSamiulHaqueHaseenaKhan．DNAFingerprintingofJuteGermplasmbyRAPD[J]．JournalofBiochemistryandMolecularBiology2002354414-419.[9]MohammadBelayatHossainAleyaAwalMohammadAminurRahmanSamiulHaqueHaseenaKhan．DistinctionbetweenCold-sensitiveand-tolerantJutebyDNAPolymorphisms[J]．JournalofBiochemistryandMolecularBiology2003365427-432.[10]A.BasuM.GhoshR.MeyerW.PowellS.L.BasakS.K.Sen．AnalysisofGeneticDiversityinCultivatedJuteDeterminedbyMeansofSSRMarkersandAFLPProfiling[J]．CropScience.200444.[11]ReyazulR.MirSachinRustgiShailendraSharma．ApreliminarygeneticanalysisoffibretraitsandtheuseofnewgenomicSSRsforgeneticdiversityinjute[J]．GuptaEuphytica.2008161413-427.[12]ARoyABandyopadhyayAKMahapatraSKGhoshNK．EvaluationofgeneticdiversityinjuteCorchorusspeciesusingSTMSISSRandRAPDmarkers[J]．Plantbreeding20061253292-297.[13].[D].2009.[14]NishatSultanaHaseenaKhanNadimAshrafM.TofaelKabirSharkar．ConstructionofanintraspecificlinkagemapofJute[J]．AsianJournalofPlantSciences.200655758-762.[15].[D].2010.[16].SRAP[D].2010.[17].[M].1999.[18].[J].1980132-33.[19]T.SahaM.GhoshS.K.Sen.Plantregenerationfromcotyledonaryexplantsofjute.CorchoruscapsularisL[J].PlantCellRe－ports1999187544-548.[20]AliM.AbbasJ.K.JonesP.D.S.Caligari.ClonalpropagationbyinvitrocultureofCorchorusjute[J].PlantCellTissueandOrganCulture19974710231-238.[21]HossainA.B.M.MAhmed.GIslamM.S.SingleandsynergisticeffectsofsomevitaminsandantioxidantsincontrollingearlysenescenceofinvitroregeneratedplantsofjuteCorchorusolitoriusL.[J].Plantphysiology-Growthanddevelopment199442123-129.[22]R.H.SarkerG.M.AminM.I.Hoque.InvitroRegenerationinThreeVarietiesofWhitejuteCorchoruscapsularisL.[J].PlantTissueCult.&Biotech.200717111-18.2364!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!![23]KMKHudaMSRBhuiyanMHKabirAFMJamalUddin.InvitroplantregenerationprotocolofTossaJuteCorchorusolitorius[J].InternationalJournalofIntegrativeBiology20071296-101.[24]HossaiAbmm．TransientGUSexpressioninJuteexplantsafterinfectionbyAgrobacteriumtumefaciens[J]．PlantTissueCult.1998811-18.[25]M.GhoshT.SahaP.NayakS.K.Sen．GenetictransformationbyparticlebombardmentofcultivatedjuteCorchoruscapsularisL[J]．PlantCellRep200220936-942.[26]AbuAshfaqurSajibMd.ShahiduIslamMd.ShamimRezaArpitaBhowmikLaylaFatemaHaseenaKhan[J]．TissuecultureindependenttransformationforCorchorusolitorius[J]．PlantCellTissueandOrganCulture2008953333-340.[27]AlversonWSWhitlockBANyffelerRBayerCBaumDA．PhylogenyofthecoreMalvalesevidencefromndhFsequencedata[J]．Am.J.Bot.199986101459-1471.[28]WhitlockBAKarolKGAlversonWS．ChloroplastDNAsequencesconfirmtheplacementoftheenigmaticOceanopapaverw