悬浮培养中不同理化因子对烟草发状根生长及烟碱合成的影响①

2005,22(5):590593ChineseBulletinofBotany(110200101005)Authorforcorrespondence.E-mail:[e-mail]:2004-05-10:2004-10-08:,1(,450002)2(100053)(Nicotianatabacum)pH5cm,40mLpH6.01.0mg.L1IAA1/2MS,2830,0.241mg.mL1,,,EffectsofVariousFactorsontheGrowthandNicotineProductionofHairyRootsofNicotianatabacuminSuspensionCulture1MUPing-Li1CUIHong1LIUHai-Jiao2ZHAOBai-Dong1(HenanAgriculturalUniversity,NationalTobaccoCultivatePhysiologyandBiochemistryResearchCenter,Zhengzhou450002)2(StateTobaccoMonopolyAdministration,Beijing100053)AbstractThegrowthandnicotinebiosynthesisofhairyrootsofNicotianatabacuminsuspensioncultureareinfluencedbyvariousfactorssuchasbasalmedium,hormonecontentandthepHvalue.Themaximalproductionofnicotineat0.241mg.mL1wasobtainedwhenonetipofhairyrootwasculturedin40mLliquid1/2MurashigeSkoogmediumsupplementedwith1.0mg.L1indolaceticacidat2628undernaturalscatteredlight.ThisresultsuggeststhepotentialapplicationofahairyrootsuspensionculturesystemfortheproductionofthesecondmetabolicsubstanceofNicotianatabacum.KeywordsNicotianatabacum,Hairyroot,Nicotine,SuspensioncultureChilton(1982)(Agrobacteriumrhizogenes),,(,5912005:1993),,(Bharati,2000),(,2003),,,11.1(Nicotianatabacum),Ar1334Sumxun,MS1.210,5cm40mL100mL,6024,51.3(Willitsetal.,1950),3(mg)=(mg)(%)+(mL)(mg.mL1)22.1MS,,,MS1/2MS1/4MS,MS,1/2MS1/4MS;310~25,25MS1/2MS,1/4MS(1)30,,MS1/2MS1/4MS3(2.12±0.71)mg(3.40±0.83)mg(1.74±0.26)mg,1/2MSMS,,,1/4MS,2.2pHpH4.06.08.01/2MS,25,2,pH8.0,(19.88±4.30)mg,pH4.0,,(5.56±1.21)mg,pH6.0,(3.96±0.89)mg,;pH8.01Fig.1Effectesofbasialmediumonhairyrootgrowth59222(5),(2.72±1.01)mg;pH4.0,2.4pH6.01/2MS,IAANAAGA3,,30,3GA3IAANAA3,GA3,0.5mg.L1121.5mg,19.1mg;IAA,1.0mg.L1;NAA,4,NAA,IAA,1.0mg.L1,0.241mg.mL1.m1,0.099mg.mL1.m1;,GA3,,GA3,3,(Sauerweinetal.,1992;,1995;,1998),,,1/2MS;(pH4),,MukundanHjortso(1991)pH,,,pH2pHFig.2EffectsofpHonnicotinsynthesisofhairyroots3Fig.3Effectsofhormoneonhairyrootgrowth4Fig.4Effectsofhormoneonnicotineoutput5932005:,,,(1995)Ri.,11:315-320,,,(2003).,9(4):26-31,(1993).,4(2):43-45,,,,,,(1998).,14:401-404BharatiG(2000)Polyaminesandplantalkaloids.In-dianJournalofExperimentBiology,38:1086-1091ChiltonMD,TepferDA,PetitA(1982)AgrobacteriumrhizogeneinsertsT-DNAintothegenomeofthehostplantcell.Nature,295:432-pH6,,;GA3IAANAA,GA3,GA3,IAA,(Rhodesetal.,1994)T-DNA,,,sumxun1.57%,,17.4%,,,,434MunkundanU,HjortsoMA(1991)GrowthandthiopheneaccumulationbyhairyrootcultureofTagetespatulainmediaofvaryinginitialpH.PlantCellReports,9:627-630RhodesMJC,ParrAJ,GiuliettiA(1994)Influenceofexogenoushormonesonthegrowthandsecondarymetaboliteformationintransformedrootcultures.PlantCell,TissueandOrganCulture,38:143-151SauerweinM,WinkM,ShimomuraK(1992)Influ-enceoflightandphytohormonesonalkaloidpro-ductionintransferredrootculturesofHyoscyausalbus.PlantPhysiology,140:147-152WillitsCO,SwainML,ConnellyJA(1950)Spectro-photometricdeterminationofnicotine.AnalyticalChemistry,22:430-433