光机二维扫描技术在激光共聚焦生物芯片扫描仪中的应用Ξ

:1005-5630(2003)04-0030-05X,,,(,310027):,f2H,,,:;;;:Q819:AAapplicationofopticalandmechanicaltwo-dimensionscanningtechnologyinthelaserconfocalbiochipscannerZHENGXu2feng,WANGLi2qiang,NIXu2xiang,LUZu2kang(CNERCforOpticalInstrument,ZhejiangUniversity,Hangzhou310027,China)Abstract:Theopticalandmechanicaltwo2dimensionscanningtechnologyappliedinthelaserconfo2calbiochipscannerisdescribedfirstlyinthispaper,onedimensionalscanningiscompletedbytheopticalscanningsystemcomposedofgalvanometerandf2Hobjectivelens,anotherisdonebythemechanicalscanningsystemmovingtheworkstagebythesteppingmotor.Andonthebaseoftheabovediscussion,thedesignofthecontrolsystemofthetwodimensionalscanningisanalyzed.Thenewandhelpfulex2perimentsaboutthelaserconfocalbiochipscannerwhichhashighscanningspeedandprecisionhavebeendone,andsomepreliminaryexperimentalresultsareachieved.Keywords:biochipscanner;opticalandmechanicaltwo2dimensionscanning;resolution;controlsystem1,,,,,,,,DNA,25420038OPTICALINSTRUMENTSVol.25,No.4August,2003X:2002203218:(19772),,,,[1,2],,,XY,:(1),,(2)XY,,,,2[3,4],XY,YY,Xf2H,,,,11A-;B-;C-;D-();E-;F-f2H;G-;H-;I-;J-;K-,A,B,C,DE,f2HFf2H,E,D,HI,IJ,K,PMTI,H,,,,,,2,2WX,R,X134:,Y,YR,X,,KT,fs,:fs=WR1TK(1),(a)f0R:f0=1T=fsKWR(2)(b)fmR:d,,fm=RT1d360=fsK360WdR2(3)(2)(3)fsKXWd,,f0fm,3[5]89C5189C2051,RS232,33,89C51,,,,89C51,,89C2051,,,f2H,X89C51,,Y,,,,,(a)2325,89C51IöO,,,(b)X,f2H:$6=fõ2H(4)f2H,X,,Kf2H,,4489C2051IöO,,E2PROME2PROM,DöAf2H,4,1,540Lm,35s140LmL=70mm,W=22mmfs=36.7kHzK=0175f0=50Hzd=1mm=118fm=400Hzf2Hf=47mmf2HNA=012540Lm334:5,,,,,,6[1],,,1[J].,2001,(5):20251[2],,1[J].(),2001,28(4):443449.[3]1[M].:,1997:3523571[4]MontaguJ1HonkanenP1FluorescenceArrayScanner[J].SPIE,1999,3779:2842921[4],,1MCS-51ö98[M].:,1998:719614325