基因芯片技术在妇科肿瘤研究中的应用

tions,technique,andresults[J].OtolaryngolHeadNeckSurg,1999,120:678682.:2004204212:(1972-),,,,,1,2(1.,523000;2.,300070)(HGP),(genechip)DNA(DNAmicroarray),,DNA,,,,cDNA[1],1,Sawiris[2],cDNA,ovachip1.1Aronow[3]cDNA6918mRNA,141,2561/2,Kabbarah[4],Amd1RNAs,,1.2HPV,HPVHPV16HPV18,HPVCho[5]22HPV685HPV,31.9%HPV,78.6%HPV165818(),HPVAn[6]HPVDNAHPVHPVDNA1983HPV,HPV18HPV58HPV16(LSILS)HPV(6113440424344)HPV58(HSILS),HPV16,Hwang[7]Kim[8]HPVDNA,DNAHPVDNA1.3Wen[9]108Tp5377,71,63DNA57,31Tp53,81%14DNA,6DNA94%92%100%,DNA87%82%100%Tp532DNA,,Liu[10]884ProceedingofClinicalMedicineJ,Jul.2004,Vol13No.7SiHa(HPV)C233A(P53)33103RF2PCR,BakC2ab1Kitahara[11]23040cDNA910,121,50,,ALDH1XRCC562,6232000,NIHcDNA60,,,[12]Lamendola[13],cDNA3SKOV23(),33:(0.003mm)(0.03mm)(0.3mm)1000,20SOM(self2organizingmap),SOM,SKOV23,1SKOV23(0.003TR)SOM,/4,,,,Casey[14]syn2decan4decorinperlecan,,,Cheng[15]385,25cDNA,NorthernRNA2RNANADH42G32C4BG30CC,S12,,S12NADH4510(KLK10),Shvartsman[16]KLK10hK10KLK10,hK10,KLK10Huang[17]XIST(X),,XIST6,,,;;;,Shmulevich[18](compositeoligonucleotidemicroarrays),,:[1]GolubTR,SlonimDK,TamagoPetal.Molecularclassifyca2tionofcancerclassdiscoveryandclasspredictionbygeneex2pressionmonitor[J].Science,1999,286:531537.[2]SawirisGP,Sherman2BaustCA,BeckerKGetal.Develop2mentofahighlyspecializedcDNAarrayforthestudayanddiagnosisofepithelialovariancancer[J].CancerRes,2002,62(10):29232928.[3]AronowBJ,RichardsonBD,HandwergerSetal.Microarrayanalysisoftrophoblastdifferentiation:geneexpressionrepor2gramminginkeygenefunctioncategories[J].PhysiolGe2nomics,2001,6:105106.[4]KabbarahO,PintoK,MutchDGetal.GoodfellowPJExpres2sionprofilingofmouseendometrialcancersmicrodissectedfromethanol2fixed,(508)98420047137,,,3.3162,,:,,:[1]KhattaS.Pregnancyoutcomefollowinggestationalexposuretoorganicsolvents[J].JAMA,1999,281(12):1106.[2]BottoLD.5,102methyleneterahydrofoiatereductasegenevari2antsandcongenitalanomalies:aHugereview[J].AmJEpi2demiol,2000,15(9):862.[3]BianchiF.Environmentandgengeticsintheetiologyofcleftlipandcleftpalatewithreferencetotheroleoffolicacid[J].EpidermiolPrev,2000,24(1):21.[4]GosenquistTH.Genesfolateandhomocysteineinembryonicdeveiopment[J].ProNutrSoc,2001,60(1):73.[5],.[J].,1993,28(2):73.[6]BottoLD.Congenitalheartdefects,maternalfebrileillness,andmuitivitaminuse:apiopulation2basedstudy[J].Epidermiolo2gy,2001,12(5):485.:2004203225:(1959-),,,,(489)paraffin2embeddedtissues[J].AmJPathol,2003,162(3):755762.[5]ChoNH,AnHJ,JeongJKetal.Genotypingof22humanpa2pillomavirustypesbyDNAchipinKoreanwomen:comparisonwithcytologicdiagnosis[J].AmJObstetGynecol,2003,188(1):5662.[6]AnHJ,ChoNH,LeeSYetal.Correlationofcervicalcarcino2maandprecancerouslesionswithhumanpapillomavirus(HPV)genotypesdetectedwiththeHPVDNAchipmicroar2raymethod[J].Cancer,2003,97(7):16721680.[7]HwangTS,JeongJK,ParkMetal.DetectionandtypingofHPVgenotypesinvariouscervicallesionsbyHPVoligonu2cleotidemicroarray[J].GynecolOncol,2003,90(1):5156.[8]KimCJ,JeongJK,ParkMetal.HPVoligonucleotidemicroar2ray2baseddetectionofHPVgenotypesincervicalneoplasticlesions[J].GynecolOncol,2003,90(2):210217.[9]WenWH,BernsteinL,LescallettJetal.ComparisonofTp53mutationsindentifiedbyoligonucleotidemicroarrayandcon2ventionalDNAsequenceanalysis[J].CancerRes,2000,60(10):27162722.[10]LiuSS,CheungAN,NganHY.Differentialgeneexpressionincervicalcancercelllinesbeforeandafterionizingradiation[J].IntJOncol,2003,22(5):10911099.[11]KitaharaO,KatagiriT,TsunodaTetal.Classificationofsen2sitivityorresistanceofcervicalcancerstoionizingradiationaccordingtoexpressionprofilesof62genesselectedbycD2NAmicroarrayanalysis[J].Neoplasia,2002,4(4):295303.[12]ScherfU,RossDT,WalthamMetal.Ageneexpressiondatabaseforthemolecularpharmacologyofcancer[J].NatGenet,2000,24(3):236244.[13]LamendolaDE,DuanZ,YusufRZetal.MoleculardescriptionofevolvingpaclitaxelresistanceintheSKOV23humanovariancarcinomacellline[J].CancerRes,2003,63(9):22002205.[14]CaseyRC,OegemaTRJr,SkubitzKMetal.Cellmembraneglycosylationmediatestheadhesion,migration,andinvasiono2fovariancarcinomacells[J].ClinExpMetastasis,2003,20(2):143152.[15]ChengQ,LauWM,TaySKetal.Identificationandcharac2terizationofgenesinvolvedinthecarcinogenesisofhumansquamouscellcervicalcarcinoma[J].IntJCancer,2002,98(3):419426.[16]ShvartsmanHS,LuKH,LeeJetal.Overexpressionofkallikrein10inepithelialovariancarcinomas[J].GynecolOncol,2003,90(1):4450.[17]HuangKC,RaoPH,LauCCetal.RelationshipofXISTex2pressionandresponsesofovariancancertochemotherapy[J].MolCancerTher,2002,1(10):769776.[18]ShmulevichI,AstolaJ,CogdellDetal.Dataextractionfromcompositeoligonucleotidemicroarrays[J].NucleicAcidsRes,2003,31(7):36.:2004203209:(1971-),,,,,805ProceedingofClinicalMedicineJ,Jul.2004,Vol13No.7