悦目金蛛和棒络新妇卵袋丝物理化学结构表征及其力学性能研究

149-163149-163(30760041)(GJJ08422GJJ08423)(2007GQN1690)(0796)[e-mail]，。、、（Argiopeamoena）（Nephilaclavata），、、。、、、、β-，、、。；；；Q957Q9560[1～3][4～9]。41(GPGXX)n／(GPGQQ)n2An／(GA)n3(GGX)n4[10]。[11]、X[12～14]、[15～17]、[18～21]。An(GA)n、β-、20102262:ACTABIOPHYSICASINICA2010Vol.26No.2:149ACTABIOPHYSICASINICA｜Vol.26No.2｜Feb.20102010262/ResearchArticle[6]β-(GGX)n(GPGXX)n(GPGQQ)n。(GGX)n310(GPGXX)n(GPGQQ)nβ[18,22]。[2,23]。9(9)、、7[23～26]。[27～30][26]、[31～33]。Casem、Foradori、、[34～37]()、、。。11.1(102.06°26.88°)。15～21mm300mg60cm×60cm×60cm(24.5±2.0)℃50%±5%L∶D=12∶12(h)。。1.21A2A。。。1.35min(JEOLJSM-5900U=20kVI=0.6nA)Smileview。150｜ACTABIOPHYSICASINICAResearchArticle/1.41～2mgL8000Hitachi,Japan。1.5KBr[39]KBr400℃4hKBr。8、、、。。Nicole170SX4,000～400cm－1。1.6LLY-06B20mm10mm/min20℃65%。90°[38]π(D0/2)2L0＝π(D/2)2LD0Dπ(D0/2)2π(D/2)2L0L。[42]。---。。SPSS13.0one-way-ANOVA。22.1SEM8μm5～12μm260～800nm12。1F2F。2.2151ACTABIOPHYSICASINICA｜Vol.26No.2｜Feb.20102010262/ResearchArticle45%56%、、。。20%6.5%22%1。。2.3800～1,700cm－12(A)(B)(C)()(D,E)()(F)()Fig.2SEMmorphologiesofN.clavataeggcasesilks(A)Eggcase;(B)Majorampullatesilkintheeggcaseframe;(C)Outercoversilk(mainlycontaininglargediametermajorampullatesilkandothersometypesofsmalldiametersilk,e.g.aciniformsilk);(D,E)Innercoversilk(containinglargediametercylindricalsilkandothersometypesofsmalldiametersilk,e.g.aciniformsilk);(F)Innercoversilk(cylindricalsilk,theapparentlongitudinalstrandsorstripeswerepresentedonitssurface)1(A)(B)(C,D)()(EF)()Fig.1SEMmorphologiesofA.amoenaeggcasesilks(A)Eggcase;(B)Majorampullatesilkintheeggcaseframe;(C,D)Graygreenandwhiteoutercoversilk(containinglargediametercylindricalandfineaciniformsilk);(E,F)Umberinnercoversilk(containingcylindricalsilk,theapparentlongitudinalstrandsorstripeswerepresentedonitssurface)152｜ACTABIOPHYSICASINICAResearchArticle/1(%)Table1comparisonofeggcasesilkanddraglineofA.amoenaandN.clavataintheaminoacidcomposition(molepercent)AminoacidsGly6.1±0.17.2±0.125.7±1.026.5±1.17.2±0.132.5±0.222.9±0.431.7±0.234.8Ala22.1±0.019.9±0.323.7±0.620.4±0.823.0±0.925.5±1.222.7±0.424.7±0.627.0Glu11.1±0.410.7±0.411.4±0.213.8±0.313.3±0.216.3±0.416.6±0.316.9±0.51.8Ser23.0±0.021.1±0.16.5±0.16.9±0.219.1±0.53.0±0.28.3±0.13.8±0.611.87Pro－－8.6±0.17.8±0.11.2±0.03.8±0.44.5±0.55.5±0.30.74Asp7.4±0.07.8±0.12.2±0.03.3±0.03.6±0.01.5±0.02.7±0.01.5±0.02.4Phe6.8±0.17.4±0.24.5±0.02.0±0.05.5±0.10.7±0.12.0±0.00.9±0.11.4Leu8.1±0.14.6±0.02.3±0.12.7±0.17.3±0.04.8±0.35.3±0.04.0±0.30.69A.amoenaInnercoversilk(n=4)Outercoversilk(n=4)Scaffoldingsilk(n=4)Dragline*(n=3)N.clavataInnercoversilk(n=4)Outeroversilk(n=4)Scaffoldingsilk(n=4)Dragline*(n=3)BombyxmoriDegumming#silkTyrThrArgValHisLysIleSilkglands4.1±0.02.0±0.14.3±0.11.9±0.01.0±0.01.5±0.1Cylindricalgland4.0±0.06.3±0.12.4±0.11.7±0.12.6±0.11.2±0.12.0±0.2－2.0±0.02.3±0.13.1±0.14.4±0.02.2±0.01.1±0.0Majorampullategland4.9±0.12.4±0.02.7±0.13.0±0.11.6±0.00.8±0.01.3±0.01.6±0.06.1±0.14.3±0.13.6±0.10.9±0.00.5±0.02.9±0.0Cylindricalgland4.1±0.40.7±0.03.1±0.01.2±0.11.6±0.10.6±0.00.6±0.03.2±0.32.1±0.03.1±0.02.3±0.31.6±0.01.1±0.01.5±0.13.7±0.30.8±0.02.6±0.01.4±0.12.1±0.00.5±0.0－9.21.20.932.80.40.250.9PostsilkglandMajorampullategland*#(2006)[43]SPSS13.0one-way-ANOVA。n*Artificiallyreeleddraglinesilk#ThedataofsilkwormsilkwerecitedfromWangetal(2006)[43]ThedatawereanalyzedwithOne-way-ANOVAprocedurefromspss13.0.nisthenumberofthespiders153ACTABIOPHYSICASINICA｜Vol.26No.2｜Feb.20102010262/ResearchArticle3(A)(B)(C)Fig.3FTIRspectraofwebsilkformtheA.amoena(A)Goldenmajorampullatesilkfromtheeggcaseframe;(B)Whitedraglinesilk(artificiallyreeled);(C)Eggcaseumberinnercoversilk、3～5。1,626cm－11,695cm－11,540cm－1960cm－1。2.42、。、、。。154｜ACTABIOPHYSICASINICAResearchArticle/4(A)(B)(C)Fig.4FTIRspectraoftheA.amoenaeggcasesilk(A)Greygreenoutercoversilk;(B)Whiteoutercoversilk;(C)Umberinnercoversilk5(A)(B)(C)Fig.5FTIRspectraofwebsilkformtheN.clavata(A)Goldenmajorampullatesilkfromtheeggcaseframe;(B)Goldendraglinesilk(naturallyspun);(C)Eggcaseinnercoversilk155ACTABIOPHYSICASINICA｜Vol.26No.2｜Feb.20102010262/ResearchArticle2Table2TensileparametersofthescaffoldingsilkandinnercoversilkoftheN.clavataandA.amoenaeggcaseSpidersilkE(GPa)εpσp(MPa)εuσu(MPa)Wf(MJm－3)A.amoenacocoonscaffoldingsilk14.0±40.031±0.008205±250.38±0.02665±70169±15A.amoenacocooninnercoversilk15.3±20.039±0.003294±180.58±0.04689±37308±12N.clavatacocoonscaffoldingsilk11.3±5.10.027±0.007185±300.26±0.07730±105107±11N.clavatacocooninnercoversilk7.0±20.039±0.003195±400.42±0.08330±60103±11N.clavatadragline9.6±20.030±0.004200±100.29±0.03785±60115±10ArgiopetrifasciatadraglineRef.406.9±0.40.013±0.00180±100.30±0.02600±5090±10AraneusviscidsilkRef.410.03－－2.7500150B.moriRef.429～170.010～0.016110～2300.10～0.20300～60050～7033.1、。[44]。[45]。(Araneusventricosus)[44]、(Argiopeargentata)[45]、(Zygiellax-notata)[46]、(Argiopebruennichi)、(Leucaugeblanda)、(Cyrtophoramoluccensis)。5～12μm260～800nmEεpσpεuσuWfE:Elasticmodulus;εp:Strainattheproportionallimitσp