药用植物淫羊藿资源可持续利用现状与展望

ChineseBulletinofBotany2009,44(3):363-370,:2008-08-04;:2008-09-17:(No.30570171)(No.999328No.995561)*E-mail:[e-mail]..1,2,2,3*,1,21,,7100622,4300743,710049,,,,,,,,,,,,,(2009)..44,363-370.(HerbaEpimedii),(Stearn,2002),(WangandHuang,2005;Naeyeretal.,2005;Zhangetal.,2008)(EpimediumL.),,,80%,(,2002),,,,11.1(benzenoid)C(Liuetal.,2004),(,2009),(Mengetal.,2005;ChenandChiu,2006;Panetal.,2007;Luetal.,2008),(,2006;,2008),36444(3)200910(1),2090,(,2003a;Ward,2004),,,(,2005)1.2,,,4(1)(Wuetal.,2003),(,2005),,(,2004;Ward,2004),,,,,,,(,2005)(Fengetal.,2006),(E.koreanum),,,,(,2008)(2),(Zhangetal.,2008),,,,(3),()(E.sagittatum)(E.wushanense)(E.brevicornu)(E.pubescens)(E.acuminatum)(E.leptorrhizum),,20(,2004),1Table1SomeChinesetraditionalpatentmedicines(Chineseproprietarymedicines)containingHerbaEpimedii365:,()(4),(,2003b)22.1(2000)1612112,,(),,;6,,,,,24,23,,,,(2003a,2004),,,,,,10,()(2003a)()Feng(2006)(),,,,,()(2002)(goodagriculturalpractice,GAP),,,(Liuetal.,2004;,2005),GAP,(2000)1;2,(2005)2(icariin),,,,;,(6),(9),(12)(2001),ZnCuMnFeCoNiSrLiMg9,Ca,,,(1),,(2)GAP(,2003b;,2005;,2008;Zhangetal.,2008),,,GAP,(3)36644(3)2009,2.2,(,2005;,2008),(2008)pH,,(,2008),,(Panaxginseng)(Fritillariacirrhosa)(Chenetal.,2006),3,,3.1GAP,,3.1.1(),(),(Shenetal.,2007;Zhangetal.,2008)DarrellProbst,,,,()(Yuetal.,2003;Rosatietal.,2004;,2006)(Saussureainvolucrata)(chalconeisomerase)(S.medusa),5,(apigenin)32.1647.8mg·L1,124(Lietal.,2006)(Glycinemax)(isoflavonesynthase)(Nicotianatabacum)(Lactucasativa)(Petuniahybrida),(genistein);-3-(flavanone3-hydroxylase),;(phenylalanineammonialyase)(Liuetal.,2007),EST(flavonolsynthase)-3-(),367:3.1.2,GAP,(epimedin)ABCC,,()3.1.3(,2001;,2005;Zhangetal.,2008),,3.2(Federicietal.,2003;LuczkiewiczandKokotkiewicz,2005;Lietal.,2006;,2008)Gao(2006)Federici(2003),(10L)60mg·L1·d1,(),Yamamoto(1992),(E.diphyllum)A(A,epimedosideA)diphyllosideBF(ikarisosideF)E(epimedosideE)ABC;Yamamoto(1997)(dimethylallyltransferase)(),(),3.3,,,(DixonandFerreira,2002;Shindoetal.,2003;Willitsetal.,2004;Jiangetal.,2005;Yanetal.,2005;,2006)Leonard(2006)(Escherichiacoli)P450(-3',5'-(flavonoid3',5'-hydroxylase)),,,,3.4,,,,(),,(Zhangetal.,2008),-36844(3)2009(,2007),3.5,,,,,,2000,(reddatabookofplantsinJapan),(Horieetal.,2007),,,,,,GAP,,,,(2001)..18,3637.,,,(2003a)..25,637638.,,,(2003b)..25,413415.,,,(2005)..24,298303.,,(2008)..25,8588.,,,(2005)..36,289295.,,,(2002).(SOP)().4,1720,3030.,,,(2000)..18,327328.,,,(2003a)..26,544545.,,,,(2003b)..38,576578.,,,,(2005)..20,670671.,,,(2004).3.29,274275.,,,(2008)..28,206210.,,,,,(2008)..26,9198.,(2008)..36,77307734.,,(2004)..29,492492.(2002).().40,481489.(2006)..6,128133.,,(2006)..37,17451751.,,(2007)..,ZL200720086099.7.2007-07-24.,(2009)..40,160163.ChenKK,ChiuJH(2006).EffectofEpimediumbrevicornumMaximextractonelicitationofpenileerectionintherat.Urol-ogy67,631635.ChenS,XiaoS,WeiJ,DaiY,XiaoP(2006).Sustainabledevel-opmentmodelofChinesemedicinalmaterialsbasedpharma-ceuticalproducts:casestudiesfrombulbulsFritillariaecirrhosaeAsia-PacTraditMed2,7275.DixonRA,FerreiraD(2002).Genistein.Phytochemistry60,205211.FedericiE,ToucheA,ChoquartS,AvantiO,FayL,OffordE,CourtoisD(2003).Highisoflavonecontentandestrogenicactivityof25year-oldGlycinemaxtissuecultures.Phytochem-istry64,717724.FengT,LiY,QianB,YangZ(2006).BiomassallocationpatternofmodulesinrametpopulationofEpimediumbrevicornu.JChinaWestNormUniv27,4247.GaoM,SongBZ,LiuCZ(2006).Dynamicmicrowave-assistedextractionofflavonoidsfromSaussureamedusaMaximcul-369:turedcells.BiochemEngJ32,7983.HorieS,SuzukiK,MakiM(2007).Characterizationofmicrosatellitelociforabarrenwortspecies(Epimediumdiphyllum,Berberidaceae).MolEcolNotes7,11721174.JiangH,WoodKV,MorganJA(2005).MetabolicengineeringofthephenylpropanoidpathwayinSaccharomycescerevisiae.ApplEnvironMicrobiol71,29622969.LeonardE,YanY,KoffasMAG(2006).FunctionalexpressionofaP450flavonoidhydroxylaseforthebiosynthesisofplant-specifichydroxylatedflavonolsinEscherichiacoli.MetabEng8,172181.LiFX,JinZP,ZhaoDX,ChengLQ,FuCX,MaF(2006).OverexpressionoftheSaussureamedusachalconeisomerasegeneinS.involucratahairyrootculturesenhancestheirbiosynthesisofapigenin.Phytochemistry67,553560.LiuCM,ZhaoXL,LiuZQ,XingJP(2004).Isolationandextrac-tionoftotalflavonoidsfromEpimediumkoreanumNakaibysupercriticalfluidextraction.ChemResChinUniv20,707710.LiuR,HuY,LiJ,LinZ(2007).Productionofsoybeanisoflavonegenisteininnon-legumeplantsviageneticallymodifiedsec-ondarymetabolismpathway.MetabEng9,17.LuY,WangD,HuY,HuangX,WangJ(2008).Sulfatedmodifi-cationofepimediumpolysaccharideandeffectsofthemodifi-ersoncellularinfectivityofIBDV.CarbohydrPolym71,180186.LuczkiewiczM,KokotkiewiczA(2005).Co-culturesofshootsandhairyrootsofGenistatinctoriaL.forsynthesisandbiotransformationoflargeamountsofphytoestrogens.PlantSci169,862871.MengFH,LiYB,XiongZL,JiangZM,LiFM(2005).Osteoblas-ticproliferativeactivityofEpimediumbrevicornumMaxim.Phytomedicine12,189193.NaeyerAD,PocockV,MilliganS,KeukeleireDD(2005).Es-trogenicactivityofapolyphenolice