聚乙烯醇-海藻酸钙-透明质酸复合水凝胶材料的含水特性-余启聪

122320080603JournalofClinicalRehabilitativeTissueEngineeringResearchJune3,2008Vol.12,No.23ISSN1673-8225CN21-1539/RCODEN:ZLKHAH44451InstituteofBiomedicalEngineering,2DepartmentofOphthalmology,SchoolofMedicine,MinistryofEducationKeyLaboratoryforRegenerativeMedicine,JinanUniversity,Guangzhou510632,GuangdongProvince,ChinaYuQi-Cong,Studyingformaster'sdegree,InstituteofBiomedicalEngineering,JinanUniversity,Guangzhou510632,GuangdongProvince,[e-mail]:LiQin-hua,Master,Associateresearcher,InstituteofBiomedicalEngineering,JinanUniversity,Guangzhou510632,GuangdongProvince,[e-mail]:theNationalNaturalScienceFoundationofChina,No.30371519*;ScienceandTechnologyPlanProjectofGuangdongProvince,No.2001A302020102*,2006B35801003*Received:2008-03-19Accepted:2008-05-08***112Watercontentofpoly(vinylalcohol)-alginateCa-hyaluronicacidhydrogelsYuQi-cong1,LiQin-hua1,ChenJian-su2AbstractBACKGROUND:Bothpoly(vinylalcohol)(PVA)andalginateCaarehydrophilicpolymer,andexhibitgooddissolution.Buttheelasticityandwatercontentratioofthematerialsarenotidealwhentheyaresingleused.OBJECTIVE:TofabricateanovelPVA-alginateCa-hyaluronicacid(HA)compositematerialasascaffoldfortissueengineering,andanalyzetheeffectonthewatercontentandswellingratioofthescaffoldpreparedwithdifferentmixingratiosofPVA,alginateNaandHA.DESIGN,TIMEANDSETTING:AcontrolledexperimentwascarriedoutinGuangdongProvincialKeyLaboratoryofBiomaterials,MinistryofEducationKeyLaboratoryforRegenerativeMedicineinJinanUniversity(Guangzhou,Guangdong,China)fromOctober2006toMarch2008.MATERIALS:PVA(Mw=14500,61500)wasproducedbyBSChemicalTechnology;PVA(Mw=88000,95000)wasproducedbyACROSORGANICS;PVA-124(Mw=24000)wastheproductofGuangzhouPharmaceuticalsCompany(China);HAwasSigmaCompany(USA);NaOH,CaCl2andalginateNawereanalyticalpure(China).METHODS:Differentweight-averagemolecularmassandalcoholysisdegreesofPVA,differentmassfractionsofalginateNaanddifferentdosesofHAwereblendedtogethertopreparescaffold.MAINOUTCOMEMEASURES:Thewatercontentandswellingratioofthecompositescaffoldweredetermined.TheeffectofPVAandHAonthestructureoftheblendswasinvestigatedunderscanningelectronmicroscope.RESULTS:ThePVA-alginateCa-HAcompositematerialwassmooth,gentleandelastic.Itswatercontentwas62.66%86.64%,andswellingratiowas145.74%324.45%.Thesetwoindexesshowedareductionwiththeincreaseofweight-averagemolecularmassandalcoholysisdegreesofPVA,andincreasedwiththeincreasingalginateNa.ButnoremarkablechangeswerefoundalongwiththeHAincrement.ScanningelectronmicroscoperesultsprovedtheincrementofPVAweight-averagemolecularmass,alginateNaandHAcouldinduceadecreaseoftheporesinthescaffold,butanetworkstructureandamicro-porousstructure,respectively.CONCLUSION:Athree-dimensionalporousPVA-alginateCa-HAscaffold,containingPVAatMw=14500and98%alcoholysisdegree,hasthehighestwatercontentandoptimalporosity.Itcanbeusedasascaffoldoftissueengineering.YuQC,LiQH,ChenJS.Watercontentofpoly(vinylalcohol)-alginateCa-hyaluronicacidhydrogels.ZhongguoZuzhiGongchengYanjiuyuLinchuangKangfu2008;12(23):4445-4449[(ps).pdf]--200610/200803Mw1450061500BSChemicalTechnologyMw8800095000ACROSORGANICS-124Mw24000SigmaNaOHCaCl2-62.66%~86.64%,145.74%~324.45%Mw1450098.[J].200812(23):4445-4449[(ps).pdf].P.O.Box1200,[e-mail][e-mail][e-mail](30371519)*(2001A302020102;2006B35801003)**:R318:A:1673-8225(2008)23-04445-052008-03-192008-05-08(54200803190009/Y·Y)[1][2-6][1-4]CaCl2,[7-10]N,[12-13],--200610/200803100mL,45,100mL0.02CaCl2,4hCa2+24h3:24h1cm1cmG60GCaCl2,Mw1450061500Mw8800095000-124Mw24000NaOHCaCl2BSChemicalTechnologyACROSORGANICSSigma.ISSN1673-8225CN21-1539/RCODEN:ZLKHAH4447:1cm1cm,V,24h,,VVV/V1003:103,0.5cm0.5cm,2.112128800088%112.2343414500615008800024000950002.32.3.1Mw145001aMw240001bMw61500880001cdMw950001e2.3.288951de981bc981a1Table1Influencesofdifferentweight-averagemolecularmassofpoly(vinylalcohol)(PVA)onwatercontentratioofthescaffold(%)14500(98%)24000(99%)61500(98%99%)88000(88%)95000(95%)PVAmolecularmass(alcoholysisdegree)71.7274.2180.3582.0385.3486.2286.8863.6375.3078.1181.1384.6583.1086.7461.8571.5277.9080.0481.8384.0384.7285.5087.3187.7487.9487.5064.2667.0069.1572.0875.8976.7279.05MassfractionsofalginateNa00.020.040.060.080.100.122Table2Influencesofdifferentweight-averagemolecularmassofpoly(vinylalcohol)(PVA)onswellingratioofthescaffold(%)14500(98%)24000(99%)61500(98%99%)88000(88%)95000(95%)PVAmolecularmass(alcoholysisdegree)229.34227.83292.86364.53404.55420.14407.44139.71249.15290.75342.14394.18386.22495.70140.25207.72230.12321.45314.02439.47428.52270.51325.36343.50294.52293.04153.92190.24190.57203.96232.52270.48238.35MassfractionsofalginateNa00.020.040.060.080.100.123Table3Influencesofhyaluronicacidonwatercontentratioofthescaffold(%)1450024000615008800095000Molecularmassofpoly(vinylalcohol)66.9163.9666.4766.5264.4165.3867.03