桑叶水提浸膏的抑菌作用研究

(200237),,,5%,pH,,60,80,;;;;AntibacteriaEffectofAqueousExtractofMulberryLeavesHuaLeiZhangWenqingZhaoXianfengEastChinaUniversityofScienceandTechnology(Shanghai200237)AbstractTostudytheantibacteriaeffectofaqueousextractofmulberryleaveson5kindsoffoodfamiliarpollu2tionbacterium.Determiningtherelativeantibacteriaactivitythroughthemethodoffilterpaperpervasion.Theresultshowedthataqueousextractofmulberryleaveshadstrongerantibacteriaactivitiesagainststaphylococcusaureusthanes2cherichiacoli,contrarilydidworseeffectonbacillussubtilis,pseudomonasfluorescenceandbacillusmegaterium.Themin2imalinhibitoryconcentrationofaqueousextractofmulberryleaveswas5%,thevariationofpHandtemperaturesignifi2cantlyaffectedtheactivities,aqueousextractofmulberryhadbetterantibacteriaactivitiesagainststaphylococcusaureusunderacidiccondition,anditsantibacteriaactivitiesdidntchangemuchunder60,relativelydeclinedobviouslyup2wardsof80.KeyWordsAqueousextractofMulberryleaves;Flavonoid;Staphylococcusaureus;Escherichiacoli;Antibacte2riaeffect,,,ATP,,,,,,,,,1DNP,,DZF-6020;(Escherichiacoli),(Bacillussubtilis),(Staphylococcusaureus),(Pseu2domonasfluorescence)(Bacillusmega2terium);;;;61200728122.19kg,7570%1:101.5h,,15%2.23g,10g,NaCl5g,20g,1000ml,pH7.27.4,12120min2.3,,,10-5,106107cfu/ml,20ml,,100l,,,5mm,,,,,37,,,11Tab1Antibacteriaeffectofaqueousextractofmulberryleavesonthetestbacteria(mm)9121,,2.4,,100l,,20ml,37,100l,100%,(MIC),2=-100%2Tab2Antibacteriarateofaqueousextractofmulberryleavesflavoneunderthedifferentconcentration(%)(%)00.250.511.522.55SA()32021314012811080302(%)0.033.4456.256065.627590.699.38EC()279245203187134947216(%)0.012.1827.2432.9751.9766.3174.1994.262,,,,MIC5%2.5pH,pH,pH5,6,7,8,(2%)pH372h,,33(2%)pHTab3Antibacteriarateofaqueousextract(2%)onstaphylo2coccusaureusunderdifferentpHvalues(%)pH5678(%)82.379.978.172.63,pH,pHpH5,2.6121,,712007281(2%)60(20min)80(20min)100(20min),,(2%),372h,,44(%)Tab4Influenceoftemperatureuponantibacteriarateforstaphylococcusaureus(%)6080100(%)75.076.346.432.2121,7mm,30.02%,4,60,,80100,,3,,,pH5,[1].,,2004,25(2):203-207[2]..,2004(12):35-37[3]..,2005,16(8):753-754[4]..(),2003,24(6):39-44[5]..,2002.5.1[6].,2004,25(5):64-66[7]..(),2000,24(3):279-282(:2006210210),,,()8(;;),,,()812007281