光电技术在生物医学中的应用_现状与发展_骆清铭

1t23004POTISC&OPLTEECOTROTICNECNHOLGOYVol.l,APrilNO.I2003:16723392(2003)010708(430074):,,,,。::;;N29:A(nItemationalBiomediealOPtiesSoeiety),BIos。BIos(sPIE)。,。。(BioPhotoniesiomedicalhPotonies)(BiomediealOitPes)。,、、。,、、、、。:,X,MIR,PET。,(OCT)、(PDT)、()、(),。《》20。,、。“”(InternationaBiomediea-optiessymposium,Bios)。,。75,27。1999102527,()“”,(SIPE)、。100,40,、、、、10。(sPIE,vol.3s63,580)。“”,13。200011810,“”,SPIE(vol.4224,430)。:2003·0226;:2003033:(1966),,,,.,863,。6。10002n1416,152。、、。200110810,“”`”。、、BirttonChance。SPIE(vol.4536,312)。20036811,()。,。1998`”,2000http:i//.bPhust.ed.ucn,2001httP:mP.hu.sted.ucn。。(),,。,、、、、、、,、、、。。。,,、,、。,、、、。、、,。,、、、。,,、。。。,,。,!、()。、,、()。2.222.1,,,、、、、、/、()、、,、,,。,,,,,,、,。1:—2200,(,。。)。,,。(NaoterMedieine2002),。,-。,、、,。,:/;,、;。,。12。,。,(PET),:1Zmm,:。PET,(,l),(),。,,PET,。(),,,,IOem(NatureReviews2002);(NauterMediicen2001)。,,,“”。Table1MainimagingteehnologiesandhteiraPPlications(NauterReviews2002)(M))),、。。(CT)))PETTT,,SPECTTT,,FRIIIFMTTT“”Bllll,,。1Tbale2CommnoimagingteehnologiesandParameters(Nautreviews0022)MMMRIII10·001mmmMihsssr,$$$$$CCCTTT50[]mmmMillll$$$$50lLmmmMillllmmmm$$$$PPPETTT1ZrnrnnnMinnn18F,llC,15000$$$$$SSSPECTTTlZmmmmMinnn99InCT(),11Iln()))$$$$FZrnrnnnSec/minnnICmmm,$$$FMTTT1ZrnrnnnSecm/innn10Cmmm$$$$BLIIIrnrMinnnCl$$$$lmmmSeC/nlinnn400mmm,,$$$$$:$10:$$1030;$$$3O/、、,。,,,,、,。,,,,。,,,NIH20002000,,。NsF002002BiophotoniesPartnerhsiPnIiitative,。3、L,、、、、。,,,。,。,600,,,,,,。,()2002《》+(seienee,nee.Zo,2002)。l:—Table3VivooPtiealimingofgeneexPressionandbiomoleeularinteractionsinlivingeells,,FFFREI,,。FRET,FFFluoreseenCeee、、RRResonaneeEnergyyy。,,。FRTTTTransferrr6,FRET,111110-A10OA。。,FRETFUMFFFLIM()()。,,FFFluoereneeLifetimeee。,FRET`FLIMIIImaginggg,。。()FRAP。FRAP,FFFRAP、。。FFFluoreseeneecove。AAAfterhPotoblehlllggg。。。()FLAP。FFFLAP:,。(FRAP)FFFluoreseeneeee〔FUP),,,LLLoealiZationlAterrr。,FLAP。PPPhootbleachinggg,。。FCS,FFFCSFluoreseeneeee。FCS(nanomolar),CCCorrelationnn,,,SSSPeetorscoPyyy、,()。FCS(ns)。。CIS,IIICSIminggg。ICSCCCorrelationnn、、。SSSPeetorscoPyyy200。,(molcearenityt)))。。,。,NIH,、。。NIH,NCI()51800,“(NTR01)”,:、、、、。NIH2000(NIBIB),,30%。20007,NIH2000,(SAIRPs),,。NSF20002002BiophotonicspartnehsripInitsative,。12.3,:l)。,、。,;,;;,,。2)。,。;;;;。:a),(、、),,;,,,,,、。、。b),,。,、、、、、、,;,,,、,();,。3)、。,、。,。31896,X[Natuer53,274(1896)〕,、。x,,,,。,?:l),eZV,;2)/;3)。,,,;,、、,;;。,,:,,(Doppler),,。。,。,,,,;,l:—,。:1)、、;2)。,(,)()。,,,。,、、,,、、。,。,、()。,,/。,、,,。,。(、、)(、)。,(NIRS,Near-Infraredspeetroscopy)x)J(、)。BeerLambert,,760lnn850mnbBqbB:△cbH=l△O·D()()l、△O·D()()〔abHoz()abH()abH02()abH()J(l)△cbH△a(D)albIoz()△a(D)abHoz(【abHoz()abH()abHoz()abH()](2),Z,.oD,。Hb02Hb,mMol,cm”。NIRS,。,。NIRS()。(),,。,。(FNI),、FNI、、,。4,。,。,,,。,,,。。、l41,,,,、,。:。,,!l[]Maki,.YYalnashit.aMayanagi,.Ewatanabe,Y.fto,andH.Koizuni,“SPatialandtemPoalranalysisofhumanmootractiviytusingnoninvasiveNIRtopography,,,Med.Phys.22(12),19972005,]995[2]B·Chacne,Q.Luo,S.Nioka.“OPtiealivnestigationofphysiolo:asutdyofintrinsieandextirnsiebiomediealeoinrast,,Phil.Trans.R.Soe.Lond.B1997,352:707716[3]B·Chance,M.eope,E.Gartton,N.RamanujiamandB.Trombegr,“Phasemeasurementoflightabsorp-tionandseerinhumantissue,,,Reviewofscientiifcinsuments,vol.69,nlo,p.34573481,1998.[4]F.F.Jobsis.`oninvasiveinfarredmonitoringofeerebriaandmyoeardiiaoxygensuiffeieneyandeicrulatoyrParameetsr,,Seience,1977,198:1264-1267[s]D.A.Boas,M.A.o’Leary,B.Chaneeetal·“ScatteringofdiffusehPotondensiytwvaesbyspheriealihnomogeneitieswithinturbidmedia:AnalytiesolutionandapPlications,,Proe.Natl-Acad.Sei.USA,1994,91:48874891[6]Q.Luo,S.Nioka,B.Chanee.“Funetionalnear-infraredimager,inoPtiealtomograPhyandsPecotrscoPyofitssue:hteoyr,insturmentation,model,andhumanstudies11”Proe.SPIE,1997,2979:8493[7]QingmingLuo,OPtiealimingineognitiveneuorseienee,SPIE,2001,4536:285292[8]RalPhWeissleder,SealingDownImaging:MoleeularMapPingofCancerInMiee,NatuerReviews,VolumeZ,Januayr2002,38AdvancesofOPtoelectroniesinBiologyandMedicineLUOQingming(TheKeyLaboartoyrofBiomediealPhotoniesofMinistyrofEdueationHuazhongUniversiytofSeieneeandeTehnologWuhan430074,China)Abstarct:ThiSPaPerrstlygivesabriintorductjonofbiomediealPhotonies.SPeeialtiesandaeademieexChangeSinthiseldaeralsointrodueed.ChaarcteristicsandadvantagesofbiomediealPhotonicaingeneexPerSSIOnandPoretinPoretinintearctionaerdiseussedindail.It15shownthatotPie1imagingbasedOnmoleeularoPtieamarking15animPortanteraltimemonitoringmethodinbjomoleeu,arphotonics·FinallopticalimagingapProaehesontissueandbarinfunotionaerintrodueed.AdvantageSofOPticalimaginginmedieineaeralsodiseussed.KeyWOrds:optoelectronies,mediealdiagnosisandthearPy,moleeularPhotonies,mediealimaging