溶藻细菌杀藻物质的研究进展

*张勇席宇吴刚**(430079):,,,:,,:Q93:A:02532654(2004)01012705*(No[2000]367)(No20026002097)**Email:wugang1962@163net:20030625,:20030925ADVANCESONALGICIDALSUBSTANCESPRODUCEDBYALGICIDALBACTERIAZHANGYongXIYuWUGang(CollegeofLifeScience,CentralChinaNormalUniversity,Wuhan430079)Abstract:Inrecentyears,moreandmoreresearchershaverealizedthepossibilitythatalgicidalbacteriacouldbeausefultoolinreducingtheimpactofharmfulalgaeblooms.Inthisreview,theecologicalrolesofalgicidalsubstanceswasbrieflydiscussed,thenspecialemphasisplacedonthecategoriesandextractmethodsofalgicidalsubstanceswhichhavebeenreported.Someideasforthefurtherstudiesonalgicidalsubstanceswerealsoproposed.Keywords:Algicidalbacteria,Harmfulalgalbloom,Algicidalsubstances,,,,(algicidalbacteria),,[1],:[2,3],,,,[4],,,,1,,127200431(1),,,:,(directattack),,;,(indirectattack),[2],,2,,(Pseudoalteromonas)21LeeA28(Pseudoalteromonassp.A28)(SkeletonemacostatumNIES324)(paperdiskassay):A28A2810,000Mw,A2810015min681h,A28,DNaseNN(NTG)A28NH1NH2NH1NH2A2815%A28,,50kD,NAlaThrProAsnAspProsuccinylAlaAlaProPhepnitroanilide,pH8830(PMSF,Ser)(DFP)EDTAEGTA,A28[2]MitsutaniA25(Pseudoalteromonassp.StrainA25)104cellsmLSkeletonemacostatumNIES324,2dA25A25W1,:A25,,,A25W1,A25A25[5]22ImamuraBiwa,16SrDNA,(Sphingomonassp.),argimicinA,C32H62N12O812ngmL100ngmL(MicrocystisviridisNIES102)(M.aeruginosaNIES298)(EscherichiacoliIAM12119)(BacillussubtilisIFO3027)(ChlorellavulgarisIAMC27)128200431(1),,[4]YamamotoL,5000ngmL[6]ArgimicinA,Banin(Vibrioshiloi),P,,,P12:PYPVYAPPPVVP,129554D,P125mmolLNH4Cl,P(10molL)5min(photosyntheticquantumyield)64%,PNH4ClP,pH7872,PNH3NH3pH,[7]P23Yoshikawa(Yap,Palau,Okinawa),2594,37(OscillatoriaamphibiaNIES361)C979,Vibriosp.,24L2216,Marfey,L(LCNAla)L,0425gmL37L,36L,L[8],24Dakhama,,,,43,,PYO(),,1[3]25Paul(Arthrobactersp.)(Chlorellavulgaris),5gmLN024gmLN10gmL[9]26,Lovejoy[10]BakerT8272B,(Thalassiosirapseudonana)[11]Shinsaku,Chattonellaantigua,129200431(1)05%,[12]HayashidaEHK1Heterocapsacircularisquama[13]Ishio(Vibrioalgoinfestus)DGI(dinoflagellatedgrowthinhibitor)Chattonellaantiqua[1],3,,,,,,Yoshikawa,,259437(OscillatoriaamphibiaNIES361)C979L[8],,Imamura,,,,,[4],,,,,4,,Imamura,,[4],[14],PCR,DNA(RNA),16SrDNAPCR16SrDNA,,,,,Kato130200431(1)A28pAS28pAS28pCRIIcA28(pASS1),106gDNAA28[15],,,,,,,,,,;,,,,,,感谢中国科学院武汉病毒研究所陈士云博士审校此文[1],,1996,20(2):173~181[2]LeeSO,KatoJ,TakiguchiN,etalApplEnvironMicrobiol,2000,66(10):4334~4339[3]DakhamaA,NoueJD,LavoieMCJApplPhycol,1993,5(9):297~306[4]ImamuraN,MotoikeI,ShimadaN,etalJAntibiotics,2001,54(7):582~587[5]MitsutaniA,YamasakiI,KitaguchiH,etalPhycologia,2001,40(3):286~291[6]YamamotoY,KouchiwaT,HodokiY,etalJAppPhycol,1998,10(2):391~397[7]BaninE,KhareSK,NaiderF,etalApplEnvironMicrobiol,2001,67(4):1536~1541[8]YoshikawaK,AdachiK,NishijimaM,etalApplEnvironMicrobiol,2000,66(2):718~722[9]PaulSB,JinnqueR,HaimBGWaterResearch,1979,13(1):267~273[10]LovejoyC,BowmanJP,HallegraeffGMApplEnvironMicrobiol,1998,64(8):2806~2813[11]BakerKH,HersonDSApplEnvironMicrobiol,1978,35(6):791~796[12]MitsutaniA,YamasakiI,KitaguchiH,etalPhycologia,2001,40(3):275~279[13]HayashidaS,TanakaS,TeramotoY,etalAgricBiolChem,1991,55(3):787~790[14]WardDM,WellerR,BatesonMMNature,1990,345(6270):63~65[15]KatoJ,AmieJ,MurataY,etalApplEnvironMicrobiol,1998,64(6):2061~2064DNARNA,EMBL()GenBank()DDBJ(),(AccessionNo),!131200431(1)