16SrRNA测序在细菌鉴定中的应用

24520069()JournalofBeijingTechnologyandBusinessUniversity(NaturalScienceEdition)Vol.24No.5Sep.2006:1671-1513(2006)05-0010-0316SrRNA龙　雯,　陈存社(北京工商大学化学与环境工程学院,北京　100037):对分离获得的一株细菌P29,经聚合酶链式反应扩增后测定该菌株的16SrRNA基因序列,由16SrRNA基因序列比较可知,菌株P29与肠杆菌属和泛菌属亲缘关系最为接近,16SrRNA基因相似性达到99%.结合生理生化实验结果综合分析后,将其鉴定为泛菌属的成团泛菌.:16SrRNA;细菌鉴定;成团泛菌:Q93-331　　　　　:A:20051213:(2004DKA3059007):(1981-),,,,;(1965-),,,,,..DNA,[1].,.(),rDNA(DNA)[2].rRNA(rDNA),16SrRNA(PCR),.P29,,16SrRNA,(Pantoeaagglomerans)..116SrRNA,,70S,50S30S,RNA.rRNA3,5S,16S23S.5S23SrRNA50S,16SrRNA30S,.16SrRNArRNADNA,.16SrRNA1540,(),K12,716SrRNA,,.16SrRNA,.rRNA,.RNA,,,,.,rRNA.,[3].16SrRNA,,.,16SrRNAPCR,16SrRNA,,,,.10,,16SrRNA.21)20059.2):,1%;,0.3%;NaCl,0.5%;,1.5%;pH,7.0.3)10g,100mL,,1010-6.,,P29,.4):(1000);:[4];16SrRNA:P2948h,PCR,PCR(50L):ddH2O38L10Buffer()5LdNTPs()(2.5mmolL-1)4L1(27f)1L2(1492r)1L,.DNA0.5LTaqE(5U/L)0.5L:6loadingBuffer()1LDNA5L:1%(1g100mL1TAE()),100mL12LGoldview(EB).:1TAE:150V,15min,DYY-33B:1492r5'-ggttaccttgttacgactt-3'27f5'-agagttgatcctggctcag-3':1OD1000LPCR:945min941min;501min;722min.257210min4PCRMJResearchPTC-200PCR.PCR,,.(0.51.0)m(13)m,,.,,30.D-,.,,,M-R..H2S,,KCN,.:L-D-.DNA:DL2000();100bpDNALadder();DNAP29()1PCR16SrRNA3.216SrRNA1,,1.5kbp.11245:16SrRNA3.316SrRNA,1.1Pantoeasp.AF39453999%Enterobactersp.AJ63985699%3.4P2916SrRNA1419bp,16SrRNA,P29,16SrRNA99%.,[4],.416SrRNA,.,16SrRNA,16SrRNA,,.P29,,..:[1].[M].:,2000.[2].[M].:,2003.[3].16SrRNAPAR[J].:,1997,24(2):61.[4],.[M].:,2001.APPLICATIONOF16SrRNASEQUENCEINBACTERIAINDICATIONLONGWen,CHENCun-she(CollegeofChemicalandEnvironmentalEngineering,BeijingTechnologyandBusinessUniversity,Beijing100037,China)Abstract:ByanalyzingaisolatedbacteriaP29physiologicalandbiochemicaltraits,morphologicalcharacteristicsandtheidentityofsequencesbetweenthisisolatedstrainand16SrRNA,itwasindicatedthattheisolatedP29wasmostsimilartoPantoeasp.andEnterobactersp..TheidentityofthemtoP29areboth99%.Bycomprehensivelyanalyzing,P29wasidentifiedtoPantoeaagglomerans,whichisbelongtoPantoeasp.Keywords:16SrRNA;identificationofbacteria;Pantoeaagglomerans(:)12()20069