精液品质相关基因的研究进展

1,2,1,1,2,1,1,1(1.,100094;2.,712100):,,,,,tsMCAKMinna53TLF,,,:;;:Q78:A:167127236(2008)0320038205,,,,,4,:2007210226:(1977-),,,,::(1950-),,Tel:[phone],E2mail:[e-mail]:(2006BAA01A10),,,,,,,,DNA,(MAS),1,DetectionoftheFecXLMutationofBMP15GeneinGoatsXIAOJie2wen1,CHUMing2xing2,LIXue2wei1,FANGLi2(1.CollegeofAnimalScienceandTechnology,SichuanAgriculturalUniversity,Yaan625014,China;2.TheKeyLaboratoryofDomesticAnimalGeneticResourcesandGermplasmInnovationofCAAS,InstituteofAnimalScience,ChineseAcademyofAgriculturalSciences,Beijing100094,China)Abstract:TheFecXLmutationofBMP15genewasdetectedinbothhighfecunditybreeds(JiningGrey,BoerandWendengDairygoats)andlowfecunditybreeds(InnerMongoliaCashmere,AngoraandLiaoningCashmeregoats)byPCR2SSCP.TheresultsindicatedthatnoneofthesixgoatbreedscarriedthesameFecXLmutation(C53Y)oftheBMP15geneasLacaunesheep.So,theFecXLmutationofBMP15genethathadsignificanteffectonfecundityofLacaunesheephadnosignificanteffectonprolificacyofJiningGreygoats.Keywords:goat;prolificacy;BMP15gene;FecXLmutation;PCR2SSCP832008353©1994-2008ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:(spermatogenesis),3::,,,;:,,,2,,,;:,,,,,,,DNA,,22.1(testisspecificmitoticcentromereassociatedkine2sin,tsMCAK)(),,,,(2003)cDNAmicroarray,(HsMCAK),(testisspe2cificMCAK,tsMCAK),tsMCAKcDNA2401bp,2013bp,671tsMCAKGenBank,AY026505,tsMCAKHsMCAK,,HsMCAK54;,tsMCAKHsMCAK(Cheng,2002)tsMCAK1P34tsMCAK,tsMCAK,tsMCAK,tsMCAKtsMCAKHsMCAK,tsMCAK,tsMCAKtsMCAK,tsMCAK2.27(testis2specificspermatogeniccellapoptosis2relatedgene,TSARG7)Tan(2005)TSARG7,cDNATSARG72463bp,1211,8p11.21456,56kD,9.13,PlsC,TSARG7mTSARG7TSARG7Au04170745697%TSARG7,TSARG7GC21932008353©1994-2008ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(Myc2inducednuclearantigenwithamolecularmassof53kD,Mina53)Tsuneoka(2006)Mina53(mina),MycMina53,Mina53Mina53Mina53Mina53,,,,Mina53(PCNA),Mina53,Mina53,Suzuki(1996),C2myc,,,2.4TATA(TATAbox2bindingprotein2relatedfactor2,Trf2)TATATBP,RNA,TATA,TBPTBP2(TBP2relatedfactor2,Trf2)TBPTrf2,,,(Zhang,2001;Martianov,2001)Trf2RNATBP,A(transcriptionfactorA,TFA)TFBTBP(TBP2associatedproteins,TAF)TFATAFTFATFA,(Ozer,2000)TAF80,TAFQTAF,,tsMCAK,,TATA,TBPTATA2box,(Kimmins,2004),TBP,,TBP,,,TATA,TBP(TLF),TBP2(TBF2)TBP(TRP),Trf2,Trf2,(Zhang,2000;Martianov,2001),Trf2,,,,Trf2,Trf2(Martianov,2002),Trf2KaltenbachRNA,Trf2,,(Kaltenbach,2000),,,TFIIATFIIBTFIIDTAF7TAF7L042008353©1994-2008ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(hormone2sensitiveli2pasegene,HSL)(TAG),,,(hormonesensitivelipase,HSL),,,,,,TAGHSL3:,2,,HSL,HSLHSL,,HSL,HSL,(Osuga,2000),HSL,Holst(1996),115.5kb,3.9kbHSLmRNA,120130kD,N2300HSLWesternBlotHSL2/2HSL,HSL(Bao,2004),HSL,,HSL2/2,HSL,2,,,1257,,(Osuga,2000;Chung,2001)WangHSL,HSL2/2,HSL2/2,2,(Wang,2004)3,,,,(Latham,1996;Furuchi,1996),,,RNA,,,(Zhu,2002;Cheng,2003;Yu,2003,2002),,RNA,,,MAS,,,1BaoS,MillerDJ,MaZ,etal.MaleMiceThatDoNotExpressGroupVIAPhospholipaseA2ProduceSpermatozoawithIm2pairedMotilityandHaveGreatlyReducedFertility.JBiolChem,2004,279(37):3819438200.2ChengLJ,LiJM,ChenJ,etal.NYD2SP16,anovelgeneas2sociatedwithspermatogenesisofhumantestis.BiolReprod,2003,68(1):190198.3ChengLJ,ZhouZM,LiJM,etal.ExpressionofanovelHsMCAKmRNAsplicevariant,tsMCAKgene,inhumantes2tis.LifeScience,2002,71(23):27412757.4ChungS,WangSP,PanL,etal.Infertilityandtesticularde2fectsinhormone2sensitivelipase2deficientmice.Endocrinology,2001,142(10):42724281.5FuruchiT,MasukoK,NishimuneY,etal.Inhibitionoftestic2142008353©1994-2008ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(6):17031709.6HolstLS,LanginD,MulderH,etal.Molecularcloning,ge2nomicorganization,andexpressionofatesticularisoformofhor2mone2sensitivelipase.Genomics,1996,35(3):441447.7KaltenbachL,HornerMA,RothmanJH,etal.TheTBPlikefactorCeTLFisrequiredtoactivateRNApolymeraseIItranscriptionduringCelegansembryogenesis.MolCell,2000,6(3):705713.8KimminsS,KotajaN,DavidsonI,etal.Testis2specifictran2scriptionmechanismspromotingmalegermcelldifferentiation.RepRod,2004,128(1):512.9LathamKE,LitvinJ,OrthJM,etal.TemporalpatternsofA2mybandB2mybgeneexpressionduringtestisdevelopment.Oncogene,1996,13(6):11611168.10MartianovI,BrancorsiniS,GansmullerA,etal.Distinctfunc2tionsofTBPandTLF/TRF2duringspermatogenesis:require2mentofTLFforheterochromaticchromocenterformationinhaploidroundspermatids.Development,2002,129(13):945955.11MartianovI,FimiaGM,DierichA,etal.Latearrestofspermat2ogenesisandgermcellapoptosisinmicelackingtheTBP2likeTLF/TRF2gene.MolCell,2001,7(3):509515.12OsugaJ,IshibashiS,OkaT,etal.Targeteddisruptionofhor2mone2sensitivelipaseresultsinmalesterilityandadipocytehy2pertrophy,butnotinobesity.ProcNatlAcadSciUSA,2000,97(2):787792.13OzerJ,MoorePA,LiebermanPM.Atestis2specifictranscrip2tionfactorATFAtau]stimulatesTATA2bindingprotein2DNAbindingandtranscriptionactivation.J