聚丙烯酰胺对活性污泥特性的影响研究

412环境工程学报Vo.l4,No.12201012ChineseJournalofEnvironmentalEngineeringDec.2010刘军平王晓昌王兴斌(,710055)PAM,PAM,,SBRPAM,FISH,PAMPAM3mg/L,,PAMMLSS,FISH,,911051810511105CFU/mL10106201051.2105CFU/mL,PAM80d,PAMCODPAMFISHSBRX703A16739108(2010)12266904StudyoneffectofpolyacrylamineoncharacteristicsofactivatedsludgeLiuJunpingWangXiaochangWangXingbin(KeyLaboratoryofNorthwestWaterResources,EnvironmentandEcology,MinistryofEducation,XianUniversityofArchitectureandTechnology,Xian710055,China)AbstractTheadditionofpolymercoagulantPAMisaneffectivewaytoenhanceparticlepelletization.However,itisstillunclearwhetherPAMhasinfluenceonthecharacteristicsofactivatedsludgeanditsbiologicaldegradationprocess.AlaboratoryexperimentwasthusconductedtoinvestigatetheeffectofcontinuousPAMadditionontheactivatedsludgeusingasetsofSBRandapplicationoffluorescenceinsituhybridization(FISH)andothermicrobiologicaltechniques.Asaresul,tnosignificantdifferencewasnoticedinthegrowthofactivatedsludgebetweenthetwoSBR,withPAMdosageof3mg/L.Moreover,certainincreaseinboththeMLSSdensityandtheamoutofmicroorganismintheweightofsludgeperunitwasfoundbyPAMaddition.TheexperimentwithFISHalsoindicatesthatthenumbersoftotalbacteria,ammoniaoxidizingbacteriaandnitrifiersweredetectedas1.0106,2.0105and1.2105CFU/mL,respectively,intheSBRwithPAMaddition,comparingwith9.1105,1.8105and1.1105CFU/mL,respectivelyintheSBRwithnoPAMaddition,whichshowsthatPAMadditiondidnotimposenegativeimpactsonmicrobialgrowth.SlightimprovementwasalsonoticedfortheremovalofCODandNH+4Nintheprocessof80doperationwithPAMaddition.Keywordspolyacrylamine;activatedsludge;fluorescenceinsituhybridization(FISH);SBR:(50778148);(2008ZX07317004):2009-10-27;:2009-12-11:(1975~),,,:Emai:[e-mail](polyacrylamide),!!!,,,,,,[1],,[2]PAM,[3],[4],,,,,DGGE2080,[57],4(fluorescenceinsituhybridization,FISH),,,PAM,,,SBR,PAM,FISH,,1112SBR,,40cm,15cm,6L,,PAMNH4ClKH2PO4%(paraformaldehyde,PFA):(85L17)(SHIMADZUUV1650PCHACHDR500)(Nikon90i)(UVPHB1000Hybridier)(MilliporeBiocel)(SHENAN)(HeraeusFresco17)11FISHTable1SpecificoligonucleotideprobesusedforFISHhybridization(5/~3/)(%)NSO1225CGCCATTGTATTACGTGTGA35Nsv443CCGTGACCGTTTCGTTCCG30NitrosospiraNtspa662GGAATTCCGCGCTCCTCT35nitrospiraNIT3CCTGGCTCCATGCTCCG40Nitrobacter(%):121.2.1配水,NH4ClKH2PO4COD300mg/L,NH+4N30mg/L,TP10mg/L1.2.2工艺运行(SBR阶段)2SBR45L,12h,5min,1675min,5025min(∀=1∀3),60L/h,30min,5min,10min,,60r/min,25#;25L,2L;,PAM(3mg/L),1.2.3微生物平板计数实验10g3g5g10g1000mL,pH75,,121#20min,45#,15mL,1mL,37#24h,(GB/T17999.42008)1.2.4FISH实验,,[89]FISH,,[10]:1mLSBR,9600xg5min,,1PBS3,1mLPFA(4%),4#3h,,1PBS,3;5,30W5min,,2h,50%80%98%(),:2mL,,46#3h,48#30min,,DAPI,4#10min,:360nm460nm221,,,COD2881mg/LNH+4N31127mg/LTP103mg/L,60L/h,A/O(1∀3)2,,25#,8d,,,;;,,,267012:,,,SBR,80mm,80d,2.2MLSS,11Fig.1Comparisonofbiomassinthereactor,2,,MLSS1700mg/L1720mg/L,,PAM,,,,,20dMLSS4104mg/L3644mg/L,,,PAM,,,MLSS,,PAM,PAM,,,,MLSS,,,23PAM2.3.1FISH计数方法,:=(1000/3)A(S/SA),:A!,S!,SA!:10,10,3,30,,;0650mm0520mm,5mm,3L,[11]2.3.2FISH计数结果(1)1.8105CFU/mL,1.1105CFU/mL,91105CFU/mL,196%118%(2)PAM20105CFU/mL,1.2105CFU/mL,1.0106CFU/mL,202%119%2.3.3FISH实验结果解析(FISH),FISH280d,,196%118%202%119%,,,;PAM,PAM242.4.1COD的去除效果COD2881mg/L,80dCOD124mg/L,COD,2,PAM12d,,COD,,PAM,PAM,,,,,,,COD;26714,PAM(15),PAM,COD2CODFig2ComparisonofCODremovalefficiency2.4.2氮的形态转变3,20d,31127mg/L,0179(PAM)0165mg/L,994%995%,,,3h,,,,18d2,PAM,,,3Fig.3ComparisonofNH+4Nremovalefficiency3(1),,25#/(3∀1),,PAM(2)PAM,COD(3)COD,,,;PAM,,PAM[1],,..,2003,6(2):4445[2],..:,2005.6468[3]LiuY.L.,WangX.C.,YuanH.L.Characterizationofmicrobialcommunitiesinafluidizedpelletbedbioreactorforwastewatertreatment.Desalination,2009,249(1):445452[4]WangX.C.,YuanH.L.,LiuY.L.,etal.Fluidisedpelletbedbioreactor,apromissingtechnologyforonsitewastewatertreatmentandreuse.WaterSc.iTechno.l,2007,55(12):5967[5],,.FISH.,2007,17(5):9495[6],..:,2004[7],,..:,2009[8]PerryOKeefeH.,S.Rigby,K.Oliveira,etal.IdentificationofindicatormicroorganismsusingastandardizedPNAFISNmethod.J.Microbio.lMethods,2006,47(3):281292[9]BruceE.Rittmann,PerryL.McCarty.Environmentalbiotechnology:Principlesandapplications.BostonBurrRidge,ILDubuque,IAMadison.WINewYork,1999[10]PerryOKeefeH.,S.Rigby,K.Oliveira,etal.IdentificationofindicatormicroorganismsusingastandardizedPNAFISNmethod.J.Microbio.lMethods,2006,47(9):281292[11],,,.FISH.,2007,33(1):22252672